A platinum glutamate acid complex as a peroxidase mimic: high activity, controllable chemical modification, and application in biosensors

Analytical Methods, Год журнала: 2024, Номер 16(7), С. 1093 - 1101

Опубликована: Янв. 1, 2024

Recent strides in nanotechnology have given rise to nanozymes, nanomaterials designed emulate enzymatic functions. Despite their promise, challenges such as batch-to-batch variability and limited atomic utilization persist. This study introduces Pt(Glu)2, a platinum glutamic acid complex, versatile small-molecule peroxidase mimic. Synthesized through straightforward method, Pt(Glu)2 exhibits robust catalytic activity stability. Steady-state kinetics reveal lower Km value compared that of natural enzymes, signifying strong substrate affinity. was explored for controllable chemical modification integration into cascade reactions with surpassing other nanomaterials. Its facile synthesis seamless enhance beyond the capabilities nanozymes. In biosensing applications, enabled simultaneous detection cholesterol alkaline phosphatase human serum high selectivity sensitivity. These findings illustrate potential small molecule mimetics catalysis biosensing, paving way broader applications.

Язык: Английский

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DNAzyme-regulated CRISPR/Cas12a based fluorescent biosensor for sensitive detection of alkaline phosphatase activity and inhibition

Analytica Chimica Acta, Год журнала: 2022, Номер 1233, С. 340518 - 340518

Опубликована: Окт. 14, 2022

Язык: Английский

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Rational Design of an Intramolecular Hydrogen Bond Enhanced Fluorescent Probe for Diagnosis of Drug-Induced Liver Injury

ACS Materials Letters, Год журнала: 2024, Номер 6(3), С. 1059 - 1068

Опубликована: Фев. 22, 2024

Drug toxicity and related drug-induced liver injury (DILI) have become major biosafety issues. Enzyme-activated fluorescent probes been demonstrated as a powerful tool for the diagnosis of DILI; however, they suffer from diffusive signal dilution interference with other organs, thus leading to misassessment drug inaccurate diagnosis. Alkaline phosphatase (ALP) is an important biomarker, alterations in enzyme level are tightly correlated severity damage. Herein, enzyme-activated intramolecular hydrogen bond (IMHB) enhanced probe (TPEG-P) was developed ALP detection cells mice models DILI. Differing previously reported intermolecular charge transfer (ICT) or excited-state proton (ESIPT) mechanisms, TPEG-P enables precise recognition imaging only through activation bonds could situ sensitively detect varying degrees caused by toxicity. This IMHB strategy will advance development bioimaging future.

Язык: Английский

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Flexible Screen‐Printed Electrochemical Sensor for Alkaline Phosphatase Detection in Biofluids for Biomedical Applications

ChemistryOpen, Год журнала: 2025, Номер unknown

Опубликована: Апрель 13, 2025

Alkaline phosphatase (ALP) is an enzyme present in the human body responsible for dephosphorylation of phosphorylated chemical species. It primarily expressed organs such as bones, liver, intestine, and placenta during pregnancy, playing a crucial role cellular processes like gene expression, transport, metabolism. Physiological ALP levels vary with age sex, normal serum ranges healthy adults between 40 190 U/L. Alterations can be indicative several pathologies, including cancer diagnosis metastasis, well bone growth dysfunctions hypophosphatasia. Conventional methods detection often require complex assay principles, extensive sample pretreatment, trained personnel. Herein, development portable, flexible electrochemical sensor fabricated through screen‐printing to monitor biological samples introduced. The sensor, characterized by high efficiency, sustainability, low cost, ease disposal, achieves limit 0.03 0.08 U/L, respectively, buffer solution samples, satisfactory repeatability lower than 10%. This simple configuration approach enables real‐time disease monitoring improves access point‐of‐care diagnostics, paving way affordable, decentralized sensors that support early better healthcare.

Язык: Английский

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Alkaline Phosphatase-Targeted, Gadolinium-Labeled Nanoparticles for Enhanced Multimodal Imaging of Liver Cancer

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер 17(19), С. 28000 - 28011

Опубликована: Май 5, 2025

Liver cancer remains one of the most lethal malignancies worldwide, primarily due to limited diagnostic and therapeutic strategies. Biological imaging agents capable selective accumulation in cancerous liver tissue offer a promising route for earlier detection improved patient outcomes. In this work, we synthesized characterized alkaline phosphatase (ALP)-targeted, gadolinium-labeled gold nanoparticles (AuNPs) designed simultaneous using magnetic resonance (MRI), computed tomography (CT), fluorescence (Fl) microscopy. The AuNPs feature 13 nm cores functionalized with ALP-binding ligands Gd(III)-macrocycles. Characterization by ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX) confirmed successful functionalization. During functionalization process, variations Gd(III) loading, surface packing density, r1 relaxivity were observed; however, high reproducibility was achieved when including methanol during AuNP labeling protocol. vitro studies HepG2 HEK293 kidney cells demonstrated cellular uptake relation ALP expression levels. Optimized conditions 10-fold increase internalization into versus cells. Further scanning (SEM) TEM on thinly sliced cell samples verified intracellular localization these nanoparticles. Collectively, findings underscore potential ALP-targeted, as versatile multimodal platform early cancer.

Язык: Английский

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