ACS Materials Letters,
Journal Year:
2024,
Volume and Issue:
6(3), P. 1059 - 1068
Published: Feb. 22, 2024
Drug
toxicity
and
related
drug-induced
liver
injury
(DILI)
have
become
major
biosafety
issues.
Enzyme-activated
fluorescent
probes
been
demonstrated
as
a
powerful
tool
for
the
diagnosis
of
DILI;
however,
they
suffer
from
diffusive
signal
dilution
interference
with
other
organs,
thus
leading
to
misassessment
drug
inaccurate
diagnosis.
Alkaline
phosphatase
(ALP)
is
an
important
biomarker,
alterations
in
enzyme
level
are
tightly
correlated
severity
damage.
Herein,
enzyme-activated
intramolecular
hydrogen
bond
(IMHB)
enhanced
probe
(TPEG-P)
was
developed
ALP
detection
cells
mice
models
DILI.
Differing
previously
reported
intermolecular
charge
transfer
(ICT)
or
excited-state
proton
(ESIPT)
mechanisms,
TPEG-P
enables
precise
recognition
imaging
only
through
activation
bonds
could
situ
sensitively
detect
varying
degrees
caused
by
toxicity.
This
IMHB
strategy
will
advance
development
bioimaging
future.
ChemistryOpen,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 13, 2025
Alkaline
phosphatase
(ALP)
is
an
enzyme
present
in
the
human
body
responsible
for
dephosphorylation
of
phosphorylated
chemical
species.
It
primarily
expressed
organs
such
as
bones,
liver,
intestine,
and
placenta
during
pregnancy,
playing
a
crucial
role
cellular
processes
like
gene
expression,
transport,
metabolism.
Physiological
ALP
levels
vary
with
age
sex,
normal
serum
ranges
healthy
adults
between
40
190
U/L.
Alterations
can
be
indicative
several
pathologies,
including
cancer
diagnosis
metastasis,
well
bone
growth
dysfunctions
hypophosphatasia.
Conventional
methods
detection
often
require
complex
assay
principles,
extensive
sample
pretreatment,
trained
personnel.
Herein,
development
portable,
flexible
electrochemical
sensor
fabricated
through
screen‐printing
to
monitor
biological
samples
introduced.
The
sensor,
characterized
by
high
efficiency,
sustainability,
low
cost,
ease
disposal,
achieves
limit
0.03
0.08
U/L,
respectively,
buffer
solution
samples,
satisfactory
repeatability
lower
than
10%.
This
simple
configuration
approach
enables
real‐time
disease
monitoring
improves
access
point‐of‐care
diagnostics,
paving
way
affordable,
decentralized
sensors
that
support
early
better
healthcare.
ACS Materials Letters,
Journal Year:
2024,
Volume and Issue:
6(3), P. 1059 - 1068
Published: Feb. 22, 2024
Drug
toxicity
and
related
drug-induced
liver
injury
(DILI)
have
become
major
biosafety
issues.
Enzyme-activated
fluorescent
probes
been
demonstrated
as
a
powerful
tool
for
the
diagnosis
of
DILI;
however,
they
suffer
from
diffusive
signal
dilution
interference
with
other
organs,
thus
leading
to
misassessment
drug
inaccurate
diagnosis.
Alkaline
phosphatase
(ALP)
is
an
important
biomarker,
alterations
in
enzyme
level
are
tightly
correlated
severity
damage.
Herein,
enzyme-activated
intramolecular
hydrogen
bond
(IMHB)
enhanced
probe
(TPEG-P)
was
developed
ALP
detection
cells
mice
models
DILI.
Differing
previously
reported
intermolecular
charge
transfer
(ICT)
or
excited-state
proton
(ESIPT)
mechanisms,
TPEG-P
enables
precise
recognition
imaging
only
through
activation
bonds
could
situ
sensitively
detect
varying
degrees
caused
by
toxicity.
This
IMHB
strategy
will
advance
development
bioimaging
future.
