Chemistry - A European Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 4, 2024
α-Cyanostilbene
represents
one
of
the
easily
functionalized
aggregation-induced
emission
(AIE)
scaffolds.
It
has
been
widely
adopted
for
construction
fluorescent
materials
broad
applications.
Here,
we
further
expanded
utilization
α-cyanostilbene
derivatives
detection
hypoxia
or
proteostasis
imbalance
in
live
cells.
Four
different
amine
containing
donors
were
introduced
to
construct
(R-ASC)
with
donor-acceptor
Equipped
cysteine
(Cys)
reactive
group,
maleimide
(MI),
R-ASC-MI
shows
fluorescence
turn-on
property
upon
binding
unfolded
proteins
vitro
and
cells
under
proteostatic
stress.
By
virtue
R-ASC-MI,
level
protein
loads
can
be
quantified
by
flow
cytometry,
visualized
microscope.
Furthermore,
also
characterized
performance
R-ASC-NO
ACS Sensors,
Год журнала:
2023,
Номер
8(10), С. 3882 - 3891
Опубликована: Сен. 22, 2023
Diabetic
cataract
(DC)
surgery
carries
risks
such
as
slow
wound
healing,
macular
edema,
and
progression
of
retinopathy
is
faced
with
a
deficiency
effective
drugs.
In
this
context,
we
proposed
protocol
to
evaluate
the
drug's
efficacy
using
lipid
droplets
(LDs)
marker.
For
purpose,
fluorescent
probe
PTZ-LD
for
LDs
detection
developed
based
on
phenothiazine
unit.
The
displays
polarity-dependent
emission
variations,
i.e.,
lower
polarity
leading
stronger
intensity.
Especially,
exhibits
photostability
superior
that
Nile
Red,
commercial
staining
dye.
Using
probe,
formation
in
DC-modeled
human
lens
epithelial
(HLE)
cells
validated,
interplay
LDs-LDs
LDs-others
are
investigated.
Unexpectedly,
transfer
between
visualized.
Moreover,
therapeutic
various
drugs
HLE
assessed.
Ultimately,
more
were
found
tissues
from
DC
patients
than
first
time.
We
anticipate
work
can
attract
attention
important
roles
during
progression.
Analytical Methods,
Год журнала:
2024,
Номер
16(24), С. 3839 - 3846
Опубликована: Янв. 1, 2024
A
mitochondria-specific
NIR
fluorescence
probe
(
CMBT
)
has
been
constructed
for
dual-detection
of
sulfur
dioxide
(SO
2
and
viscosity
with
high
selectivity
sensitivity
in
living
cells
mice.
Analytical Chemistry,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 14, 2024
Diabetes
is
a
chronic
disease
marked
by
high
blood
glucose.
With
the
progress
of
diabetes,
complications
gradually
appear,
and
various
organs
may
be
affected.
However,
due
to
lack
noninvasive
in
situ
detection
probes,
diagnosis
organ
damage
caused
diabetes
significantly
delayed,
which
will
cause
many
that
cannot
treated
time.
Here,
we
report
BODIPY-based
fluorescent
probe
SNL,
can
used
detect
lung
liver
diabetes.
By
introducing
methylpiperazine
extending
conjugated
system,
SNL
locate
lysosomes
exhibit
absorption
emission
both
near-infrared
(NIR)
region.
In
addition,
sensitive
polarity
for
lysosomal
changes.
Unexpectedly,
targets
images
lungs
mice.
Subsequently,
hyperglycemia-stimulated
cell
models
diabetic
mouse
were
successfully
established,
was
utilized
reveal
as
diagnostic
signal
complications.
Notably,
first
time
confirmed
injury
using
probes
method,
providing
new
approach
