Versatile organic fluorescent probes for visualizing the dynamics of lipid droplets

Coordination Chemistry Reviews, Год журнала: 2024, Номер 522, С. 216181 - 216181

Опубликована: Сен. 5, 2024

Язык: Английский

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Polarity-Driven Fluorescence Monitoring of Lipid Droplet Dynamics in Dry Eye Disease

Analytical Chemistry, Год журнала: 2024, Номер 96(24), С. 9975 - 9983

Опубликована: Июнь 3, 2024

The emergence of lipid droplets (LDs) has been recognized as cellular markers ocular surface hyperosmosis, which is a fundamental mechanism driving dry eye disease (DED), while their dynamics during DED progression and therapy remains unlocked. For this purpose, an LD-specific fluorescent probe P1 presented in work that exhibits highly selective sensitive emission enhancement response to decreased ambient polarity (Δf) from 0.209 0.021. hydrophobic nature enables specific staining LDs, facilitating visualization changes within these structures. Utilizing P1, we observe decrease accompanied by increase the size number LDs hyperosmotic human corneal epithelial cells (HCECs). Furthermore, interplays between organelles such mitochondria Golgi apparatus are visualized, suggesting underlying pathogenesis DED. Notably, variations observed after inhibition ferroptosis or activation autophagy HCECs, implying great potential indicators for design efficacy evaluation drugs regarding targets. Finally, confirmed be overproduced tissues mice, application clinical drops effectively impedes changes. This detailed exploration underscores significant roles indicator deep insight into advancement therapy.

Язык: Английский

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Mechanistic analysis of viscosity-sensitive fluorescent probes for applications in diabetes detection

Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(12), С. 2917 - 2937

Опубликована: Янв. 1, 2024

This review focuses on fluorescence mechanisms for viscosity responsiveness, highlighting some important probes like coumarin, BODIPY, and xanthene as well the application of these viscosity-sensitive successful diabetes detection.

Язык: Английский

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A Solvatochromic and Photosensitized Lipid Droplet Probe Detects Local Polarity Heterogeneity and Labels Interacting Proteins in Human Liver Disease Tissue

Advanced Healthcare Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

Abstract The intricate morphology, physicochemical properties, and interacting proteins of lipid droplets (LDs) are associated with cell metabolism related diseases. To uncover these layers information, a solvatochromic photosensitized LDs‐targeted probe based on the furan‐based D‐D‐π‐A scaffold is developed to offer following integrated functions. First, turn‐on fluorescence upon selectively binding LDs allows for direct visualization their location morphology. Second, its linear correlation polarity quantifies micro‐environmental heterogeneity among LDs. Third, unique properties enable photocatalytic proximity labeling enrichment LDs‐interacting proteins, ready potential downstream proteomic analysis. These functions exemplified using artificial in buffer, stressed liver line, diseased tissues biopsied from patients. While most LD sensors only imaging functions, multi‐functional reported herein integrates both singlet triplet photosensitization studies.

Язык: Английский

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A polarity-sensitive fluorescent probe for visualizing lipid droplets in ferroptosis, cuproptosis, and autophagy

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Год журнала: 2025, Номер 332, С. 125854 - 125854

Опубликована: Фев. 3, 2025

Язык: Английский

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Precise diabetes visualization designed for lipid droplet-targeted polarity-sensitive fluorescent probe

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113018 - 113018

Опубликована: Фев. 1, 2025

Язык: Английский

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Exploiting Flavylium Merocyanine Dyes for Intrinsic, Multiplexed Labeling of the Endoplasmic Reticulum

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Merocyanine dyes are a versatile class of donor-acceptor polymethine that exhibit unique properties depending on their structural makeup and surrounding environment. Scaffolds favor the cyanine state (i.e., narrow, red-shifted absorption high fluorescence quantum yields) in biologically relevant settings highly advantageous for multiplexed labeling experiments, but remain limited by visible absorption. Herein, we synthesize new far-red (650-700 nm) to near-infrared (NIR, 700-1000 flavylium merocyanine demonstrate that, unlike conventional scaffolds, they with increasing solvent viscosity hydrogen bond donation, rather than polarity. We leverage these live cell labeling, where observed intrinsic targeting endoplasmic reticulum (ER) lipid droplets, minimal crosstalk commercial stains. reveal ER is achieved dipolarity lipophilicity (ClogP) architecture, making this simple, alternative more structurally complex stains currently available.

Язык: Английский

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Recent advances and prospects of nanoparticle-based drug delivery for diabetic ocular complications

Theranostics, Год журнала: 2025, Номер 15(8), С. 3551 - 3570

Опубликована: Фев. 25, 2025

Diabetes mellitus (DM) is a chronic metabolic disorder that significantly affects various organ systems. The systemic effects of DM lead to numerous complications, with ocular manifestations being particular concern due their severity and impact on quality life. Hyperglycemia-induced damage often results in range lesions, including diabetic retinopathy (DR), keratopathy, cataracts, glaucoma. These conditions impose considerable physical discomfort patients place substantial economic burden healthcare advent nanotechnology has facilitated the development innovative therapeutic strategies for managing complications. This review highlights several common complications associated DM, focusing pathogenesis treatment strategies. Emphasis placed applications potential treating

Язык: Английский

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Recent advances in fluorescent probes for bioimaging lipid droplets associated diseases

Dyes and Pigments, Год журнала: 2025, Номер unknown, С. 112792 - 112792

Опубликована: Март 1, 2025

Язык: Английский

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Rational Design of a Highly Sensitive Carboxylesterase Probe and Its Application in High-Throughput Screening for Uncovering Carboxylesterase Inhibitors

The Journal of Organic Chemistry, Год журнала: 2024, Номер 89(20), С. 14650 - 14657

Опубликована: Май 8, 2024

Tracking carboxylesterases (CESs) through noninvasive and dynamic imaging is of great significance for diagnosing treating CES-related metabolic diseases. Herein, three BODIPY-based fluorescent probes with a pyridine unit quaternarized via an acetoxybenzyl group were designed synthesized to detect CESs based on the photoinduced electron transfer process. Notably, among these probes, BDPN2-CES exhibited remarkable 182-fold fluorescence enhancement within 10 min. Moreover, successfully enabled real-time endogenous CES variations in living cells. Using BDPN2-CES, visual high-throughput screening method inhibitors was established, culminating discovery efficient inhibitor, WZU-13, sourced from chemical library. These findings suggest that could provide new avenue diseases, WZU-13 emerges as promising therapeutic candidate CES-overexpression pathological processes.

Язык: Английский

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