International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(8), С. 4386 - 4386
Опубликована: Апрель 15, 2022
Bacterial
biofilm
represents
a
multicellular
community
embedded
within
an
extracellular
matrix
attached
to
surface.
This
lifestyle
confers
bacterial
cells
protection
against
hostile
environments,
such
as
antibiotic
treatment
and
host
immune
response
in
case
of
infections.
The
Pseudomonas
genus
is
characterised
by
species
producing
strong
biofilms
difficult
be
eradicated
extraordinary
metabolic
versatility
which
may
support
energy
carbon/nitrogen
assimilation
under
multiple
environmental
conditions.
Nutrient
availability
can
perceived
which,
turn,
readapts
its
metabolism
finally
tune
own
formation
dispersion.
A
growing
number
papers
now
focusing
on
the
mechanism
nutrient
perception
possible
strategy
weaken
barrier
cues.
One
most
important
nutrients
amino
acid
L-arginine,
crucial
metabolite
sustaining
growth
both
carbon
nitrogen
source.
Under
low-oxygen
conditions,
L-arginine
also
serve
for
ATP
production,
thus
allowing
bacteria
survive
anaerobic
environments.
has
been
associated
with
biofilms,
virulence,
resistance.
key
precursor
regulatory
molecules
polyamines,
whose
involvement
homeostasis
reported.
Given
biomedical
biotechnological
relevance
control,
state
art
effects
mediated
modulation
presented,
special
focus
biofilm.
Possible
applications
are
discussed.
Cell,
Год журнала:
2021,
Номер
184(23), С. 5740 - 5758.e17
Опубликована: Ноя. 1, 2021
Biofilms
are
community
architectures
adopted
by
bacteria
inclusive
of
a
self-formed
extracellular
matrix
that
protects
resident
from
diverse
environmental
stresses
and,
in
many
species,
incorporates
DNA
(eDNA)
and
DNABII
proteins
for
structural
integrity
throughout
biofilm
development.
Here,
we
present
evidence
this
eDNA-based
architecture
relies
on
the
rare
Z-form.
Z-form
accumulates
as
biofilms
mature
through
stabilization
proteins,
confers
to
matrix.
Indeed,
substances
known
drive
B-DNA
into
Z-DNA
promoted
formation
whereas
those
disrupted
extant
biofilms.
Importantly,
demonstrated
universal
bacterial
family
stabilizes
both
bacterial-
host-eDNA
situ.
A
model
is
proposed
role
pathogenesis,
innate
immune
response,
evasion.
International Journal of Molecular Sciences,
Год журнала:
2021,
Номер
22(16), С. 9100 - 9100
Опубликована: Авг. 23, 2021
After
the
first
ancient
studies
on
microbial
slime
(the
name
by
which
biofilm
matrix
was
initially
indicated),
multitudes
of
morphology,
composition
and
physiology
biofilms
have
arisen.
The
emergence
role
that
play
in
pathogenesis
recalcitrant
persistent
clinical
infections,
such
as
periprosthetic
orthopedic
has
reinforced
scientific
interest.
Extracellular
DNA
(eDNA)
is
a
recently
uncovered
component
proving
to
be
almost
omnipresent
extracellular
polymeric
substance
(EPS)
biofilm.
This
macromolecule
eliciting
unprecedented
consideration
for
critical
impact
chronic
infections.
systematic
review
literature,
an
updated
description
eDNA
presented,
with
special
focus
latest
findings
regarding
its
fundamental
structural
contribution
it
makes
complex
architecture
bacterial
through
interactions
variety
other
molecular
components
matrix.
Foods,
Год журнала:
2021,
Номер
10(9), С. 2117 - 2117
Опубликована: Сен. 8, 2021
Biofilm
formation
is
an
integral
part
of
the
microbial
life
cycle
in
nature.
In
food
processing
environments,
bacterial
transmissions
occur
primarily
through
raw
or
undercooked
foods
and
by
cross-contamination
during
unsanitary
preparation
practices.
Foodborne
pathogens
form
biofilms
as
a
survival
strategy
various
unfavorable
which
also
become
frequent
source
recurrent
contamination
outbreaks
foodborne
illness.
Instead
focusing
on
biofilm
their
pathogenicity
individually,
this
review
discusses
molecular
level
how
these
two
physiological
processes
are
connected
several
common
such
Listeria
monocytogenes,
Staphylococcus
aureus,
Salmonella
enterica
Escherichia
coli.
addition,
Pseudomonas
aeruginosa
discussed
because
it
aids
persistence
many
forming
polymicrobial
contact
surfaces,
thus
significantly
elevating
safety
public
health
concerns.
Furthermore,
in-depth
analyses
molecules
with
dual
functions
highlighted.
Biotechnology and Bioengineering,
Год журнала:
2021,
Номер
118(6), С. 2129 - 2141
Опубликована: Март 22, 2021
Advances
in
biotechnology
to
treat
and
cure
human
disease
have
markedly
improved
health
the
development
of
modern
societies.
However,
substantial
challenges
remain
overcome
innate
biological
factors
that
thwart
activity
efficacy
pharmaceutical
therapeutics.
Until
recently,
importance
extracellular
DNA
(eDNA)
biofilms
was
overlooked.
New
data
reveal
its
extensive
role
biofilm
formation,
adhesion,
structural
integrity.
Different
approaches
target
eDNA
as
anti-biofilm
therapies
been
proposed,
but
corresponding
barriers
are
still
difficult
disrupt.
Therefore,
more
creative
eradicate
needed.
The
production
often
originates
with
genetic
material
bacterial
cells
through
cell
lysis.
genomic
not
necessarily
structurally
or
compositionally
identical.
Variations
noteworthy
because
they
dictate
important
interactions
within
biofilm.
Interactions
between
components
may
well
be
exploited
alternative
strategies.
In
this
review,
we
discuss
recent
developments
research,
emphasizing
potential
ways
disrupt
biofilms.
This
review
also
highlights
proteins,
exopolysaccharides,
other
molecules
interacting
can
serve
therapeutic
targets.
Overall,
array
diverse
is
structure,
architecture,
stability.
