Identifying behavioral structure from deep variational embeddings of animal motion

Communications Biology, Год журнала: 2022, Номер 5(1)

Опубликована: Ноя. 18, 2022

Abstract Quantification and detection of the hierarchical organization behavior is a major challenge in neuroscience. Recent advances markerless pose estimation enable visualization high-dimensional spatiotemporal behavioral dynamics animal motion. However, robust reliable technical approaches are needed to uncover underlying structure these data segment into discrete hierarchically organized motifs. Here, we present an unsupervised probabilistic deep learning framework that identifies from variational embeddings motion (VAME). By using mouse model beta amyloidosis as use case, show VAME not only motifs, but also captures representation motif’s usage. The approach allows for grouping motifs communities differences community-specific motif usage individual cohorts were undetectable by human visual observation. Thus, segmentation applicable wide range experimental setups, models conditions without requiring supervised or a-priori interference.

Язык: Английский

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Two-photon calcium imaging of neuronal activity

Nature Reviews Methods Primers, Год журнала: 2022, Номер 2(1)

Опубликована: Сен. 1, 2022

Язык: Английский

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Miniscope-LFOV: A large-field-of-view, single-cell-resolution, miniature microscope for wired and wire-free imaging of neural dynamics in freely behaving animals

Science Advances, Год журнала: 2023, Номер 9(16)

Опубликована: Апрель 21, 2023

Imaging large-population, single-cell fluorescent dynamics in freely behaving animals larger than mice remains a key endeavor of neuroscience. We present large-field-of-view open-source miniature microscope (MiniLFOV) designed for large-scale (3.6 mm × 2.7 mm), cellular resolution neural imaging rats. It has an electrically adjustable working distance up to 3.5 ± 100 μm, incorporates absolute head orientation sensor, and weighs only 13.9 g. The MiniLFOV is capable both deep brain cortical been validated rats by simultaneously >1000 GCaMP7s-expressing neurons the hippocampal CA1 layer head-fixed ~2000 dorsal cortex through cranial window. also supports optional wire-free operation using novel, data acquisition expansion board. expect that this new implementation UCLA Miniscope platform will enable researchers address novel hypotheses concerning function animals.

Язык: Английский

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Basal forebrain cholinergic signalling: development, connectivity and roles in cognition

Nature reviews. Neuroscience, Год журнала: 2023, Номер 24(4), С. 233 - 251

Опубликована: Фев. 23, 2023

Язык: Английский

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Simultaneous, cortex-wide dynamics of up to 1 million neurons reveal unbounded scaling of dimensionality with neuron number

Neuron, Год журнала: 2024, Номер 112(10), С. 1694 - 1709.e5

Опубликована: Март 6, 2024

Язык: Английский

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Interfacing with the Brain: How Nanotechnology Can Contribute

ACS Nano, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Interfacing artificial devices with the human brain is central goal of neurotechnology. Yet, our imaginations are often limited by currently available paradigms and technologies. Suggestions for brain-machine interfaces have changed over time, along technology. Mechanical levers cable winches were used to move parts during mechanical age. Sophisticated electronic wiring remote control arisen age, ultimately leading plug-and-play computer interfaces. Nonetheless, brains so complex that these visions, until recently, largely remained unreachable dreams. The general problem, thus far, most technology mechanically and/or electrically engineered, whereas a living, dynamic entity. As result, worlds difficult interface one another. Nanotechnology, which encompasses engineered solid-state objects integrated circuits, excels at small length scales single few hundred nanometers and, thus, matches sizes biomolecules, biomolecular assemblies, cells. Consequently, we envision nanomaterials nanotools as opportunities in alternative ways. Here, review existing literature on use nanotechnology look forward discussing perspectives limitations based authors' expertise across range complementary disciplines─from neuroscience, engineering, physics, chemistry biology medicine, science mathematics, social jurisprudence. We focus but also include information from related fields when useful complementary.

Язык: Английский

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A three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice

Nature Methods, Год журнала: 2022, Номер 20(4), С. 610 - 616

Опубликована: Ноя. 28, 2022

Abstract Advances in head-mounted microscopes have enabled imaging of neuronal activity using genetic tools freely moving mice but these are restricted to recording minimally lit arenas and upper cortical layers. Here we built a 2-g, three-photon excitation-based microscope, containing z -drive that access all layers while behaved fully environment. The microscope had on-board photon detectors, robust environmental light, the arena lighting was timed end each line-scan, enabling functional from layer 4 6 neurons expressing jGCaMP7f roaming or dark arena. By comparing measured populations show is differentially modulated by conditions during free exploration.

Язык: Английский

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Multiregion neuronal activity: the forest and the trees

Nature reviews. Neuroscience, Год журнала: 2022, Номер 23(11), С. 683 - 704

Опубликована: Окт. 3, 2022

Язык: Английский

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Imagining the future of optical microscopy: everything, everywhere, all at once

Communications Biology, Год журнала: 2023, Номер 6(1)

Опубликована: Окт. 28, 2023

The optical microscope has revolutionized biology since at least the 17

Язык: Английский

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Behavioral decomposition reveals rich encoding structure employed across neocortex in rats

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Июль 4, 2023

Abstract The cortical population code is pervaded by activity patterns evoked movement, but it remains largely unknown how such signals relate to natural behavior or they might support processing in sensory cortices where have been observed. To address this we compared high-density neural recordings across four regions (visual, auditory, somatosensory, motor) relation modulation, posture, and ethograms of freely foraging male rats. Momentary actions, as rearing turning, were represented ubiquitously could be decoded from all sampled structures. However, more elementary continuous features, pose followed region-specific organization, with neurons visual auditory preferentially encoding mutually distinct head-orienting features world-referenced coordinates, somatosensory motor principally the trunk head egocentric coordinates. tuning properties synaptically coupled cells also exhibited connection suggestive area-specific uses movement signals, particularly regions. Together, our results indicate that ongoing encoded at multiple levels throughout dorsal cortex, low-level are differentially utilized different serve locally relevant computations.

Язык: Английский

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