Engineering Microbiology, Год журнала: 2025, Номер unknown, С. 100191 - 100191
Опубликована: Фев. 1, 2025
Язык: Английский
Cell, Год журнала: 2024, Номер 187(5), С. 1076 - 1100
Опубликована: Фев. 1, 2024
Genome editing has been a transformative force in the life sciences and human medicine, offering unprecedented opportunities to dissect complex biological processes treat underlying causes of many genetic diseases. CRISPR-based technologies, with their remarkable efficiency easy programmability, stand at forefront this revolution. In Review, we discuss current state CRISPR gene technologies both research therapy, highlighting limitations that constrain them technological innovations have developed recent years address them. Additionally, examine summarize landscape applications context health therapeutics. Finally, outline potential future developments could shape coming years.
Язык: Английский
Процитировано
119
Cell Reports, Год журнала: 2023, Номер 42(7), С. 112672 - 112672
Опубликована: Июнь 21, 2023
Prokaryotic adaptation is strongly influenced by the horizontal acquisition of beneficial traits via mobile genetic elements (MGEs), such as viruses/bacteriophages and plasmids. However, MGEs can also impose a fitness cost due to their often parasitic nature differing evolutionary trajectories. In response, prokaryotes have evolved diverse immune mechanisms against MGEs. Recently, our understanding abundance diversity prokaryotic systems has greatly expanded. These defense degrade invading material, inhibit genome replication, or trigger abortive infection, leading population protection. this review, we highlight these strategies, focusing on most recent discoveries. The study defenses not only sheds light microbial evolution but uncovers novel enzymatic activities with promising biotechnological applications.
Язык: Английский
Процитировано
65
Nature Biotechnology, Год журнала: 2023, Номер 42(5), С. 745 - 757
Опубликована: Июнь 29, 2023
Язык: Английский
Процитировано
46
Cell, Год журнала: 2024, Номер 187(13), С. 3249 - 3261.e14
Опубликована: Май 22, 2024
Thermostable clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas9) enzymes could improve genome-editing efficiency delivery due to extended protein lifetimes. However, initial experimentation demonstrated Geobacillus stearothermophilus Cas9 (GeoCas9) be virtually inactive when used in cultured human cells. Laboratory-evolved variants of GeoCas9 overcome this natural limitation by acquiring mutations the wedge (WED) domain that produce >100-fold-higher levels. Cryoelectron microscopy (cryo-EM) structures wild-type improved (iGeoCas9) reveal contacts between WED iGeoCas9 DNA substrates. Biochemical analysis shows accelerates unwinding capture substrates under magnesium-restricted conditions typical mammalian but not bacterial These findings enabled rational engineering other orthologs enhance levels, pointing a general strategy for editing enzyme improvement. Together, these results uncover new role demonstrate how accelerated target dramatically improves Cas9-induced activity.
Язык: Английский
Процитировано
21
Cell Host & Microbe, Год журнала: 2024, Номер 32(6), С. 875 - 886.e9
Опубликована: Май 15, 2024
Язык: Английский
Процитировано
18
Molecular Cell, Год журнала: 2023, Номер 83(15), С. 2768 - 2780.e6
Опубликована: Июль 3, 2023
Язык: Английский
Процитировано
40
Current Opinion in Microbiology, Год журнала: 2023, Номер 73, С. 102321 - 102321
Опубликована: Апрель 28, 2023
Язык: Английский
Процитировано
31
Military Medical Research, Год журнала: 2023, Номер 10(1)
Опубликована: Март 10, 2023
Abstract The rapid development of genome editing technology has brought major breakthroughs in the fields life science and medicine. In recent years, clustered regularly interspaced short palindromic repeats (CRISPR)-based toolbox been greatly expanded, not only with emerging CRISPR-associated protein (Cas) nucleases, but also novel applications through combination diverse effectors. Recently, transposon-associated programmable RNA-guided systems have uncovered, adding myriads potential new tools to toolbox. CRISPR-based revolutionized cardiovascular research. Here we first summarize advances involving newly identified Cas orthologs, engineered variants systems, then discuss CRISPR-Cas precise editing, such as base prime editing. We highlight progress research using technologies, including generation genetically modified vitro animal models diseases (CVD) well treating different types CVD. Finally, current limitations future prospects technologies are discussed.
Язык: Английский
Процитировано
30
Current Issues in Molecular Biology, Год журнала: 2023, Номер 45(4), С. 3705 - 3732
Опубликована: Апрель 21, 2023
Elucidation of the tertiary structure proteins is an important task for biological and medical studies. AlphaFold, a modern deep-learning algorithm, enables prediction protein to high level accuracy. It has been applied in numerous studies various areas biology medicine. Viruses are entities infecting eukaryotic procaryotic organisms. They can pose danger humans economically significant animals plants, but they also be useful control, suppressing populations pests pathogens. AlphaFold used molecular mechanisms viral infection facilitate several activities, including drug design. Computational analysis bacteriophage receptor-binding contribute more efficient phage therapy. In addition, predictions discovery enzymes origin that able degrade cell wall bacterial The use assist fundamental research, evolutionary ongoing development improvement ensure its contribution study will future.
Язык: Английский
Процитировано
23
Analytical Chemistry, Год журнала: 2024, Номер 96(26), С. 10654 - 10661
Опубликована: Июнь 14, 2024
The trans-cleavage properties of Cas12a make it important for gene editing and disease diagnosis. In this work, the effect spatial site resistance on activity was studied. First, we have explored cutting when different-sized nanoparticles are linked with various spacings DNA strands using fluorescence method. minimum spacing that cas12a can cut determined. We found size increases, gradually increases. Subsequently, verified conclusion surface-enhanced Raman scattering (SERS) method, at same time, designed a SERS biosensor achieve ultrasensitive detection P53 linear range 1 fM-10 nM limit 0.40 fM. Our work develops deep study gives guide design in cas12a-related studies, which be applied biomedical analysis other fields.
Язык: Английский
Процитировано
9