Trends in Plant Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
Transposable
elements
(TEs)
constitute
a
large
portion
of
many
plant
genomes
and
play
important
roles
in
regulating
gene
expression
driving
genome
evolution
crop
domestication.
Despite
advances
understanding
the
functions
mechanisms
TEs,
comprehensive
review
their
integrated
knowledge
cutting-edge
biotechnological
applications
TEs
is
still
needed.
We
provide
thorough
overview
that
connects
discoveries,
mechanisms,
technologies
associated
with
TEs.
discuss
identification
function
driven
by
functional
genomics,
epigenetic
regulation
utilization
active
genomics
engineering.
In
summary,
expanding
application
will
be
beneficial
to
breeding
synthetic
biology
future.
Cell Research,
Год журнала:
2024,
Номер
34(9), С. 630 - 647
Опубликована: Июль 5, 2024
Abstract
Mutations
in
amino
acid
sequences
can
provoke
changes
protein
function.
Accurate
and
unsupervised
prediction
of
mutation
effects
is
critical
biotechnology
biomedicine,
but
remains
a
fundamental
challenge.
To
resolve
this
challenge,
here
we
present
Pro
tein
M
utational
E
ffect
P
redictor
(ProMEP),
general
multiple
sequence
alignment-free
method
that
enables
zero-shot
effects.
A
multimodal
deep
representation
learning
model
embedded
ProMEP
was
developed
to
comprehensively
learn
both
structure
contexts
from
~160
million
proteins.
achieves
state-of-the-art
performance
mutational
effect
accomplishes
tremendous
improvement
speed,
enabling
efficient
intelligent
engineering.
Specifically,
accurately
forecasts
consequences
on
the
gene-editing
enzymes
TnpB
TadA,
successfully
guides
development
high-performance
tools
with
their
engineered
variants.
The
efficiency
5-site
mutant
reaches
up
74.04%
(vs
24.66%
for
wild
type);
base
editing
tool
basis
TadA
15-site
(in
addition
A106V/D108N
double
renders
deoxyadenosine
deaminase
activity
TadA)
exhibits
an
A-to-G
conversion
frequency
77.27%
69.80%
ABE8e,
previous
TadA-based
adenine
editor)
significantly
reduced
bystander
off-target
compared
ABE8e.
not
only
showcases
superior
predicting
proteins
also
demonstrates
great
capability
guide
Therefore,
exploration
gigantic
space
facilitates
practical
design
proteins,
thereby
advancing
studies
biomedicine
synthetic
biology.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 5, 2025
Abstract
Single‐atom
nanozymes
(SAzymes),
with
their
superior
enzyme‐like
catalytic
activity,
have
emerged
as
promising
candidates
for
oncology
therapeutics.
The
well‐defined
structures
of
SAzymes
make
them
well
predictable
by
experiences
and
theoretical
calculation.
However,
the
effects
metal
center
species
coordination
environments
on
activity
are
variable,
screening
artificial
experiments
is
challenging.
High‐throughput
can
rapidly
select
optimal
thus
better
application
in
tumor
therapy
highly
desirable.
Herein,
a
“high‐throughput
screening‐SAzymes
structures”
system
established
efficient
drug
preparation
density
functional
theory
oxidase‐like
processes
screened
differences
brought
about
different
metals
environments.
Through
this
process,
transition
(Mn,
Fe,
Co,
Ni)
active
centers
synthesized
then
tested
multi‐enzyme
activities.
It
found
that
SAzyme
Co
exhibited
best
further
showed
good
anti‐oral
squamous
cell
carcinoma
properties
both
vitro
vivo.
This
study
opens
up
new
avenue
rational
design
oral
cancer
combining
computational
experimental
validation.
Molecular Therapy,
Год журнала:
2023,
Номер
32(1), С. 32 - 43
Опубликована: Ноя. 11, 2023
In
2012,
it
was
discovered
that
precise
gene
editing
could
be
induced
in
target
DNA
using
the
reprogrammable
characteristics
of
CRISPR
system.
Since
then,
several
studies
have
investigated
potential
system
to
edit
various
biological
organisms.
For
typical
obtained
from
bacteria
and
archaea,
many
application
been
conducted
spread
fields.
To
date,
orthologs
with
other
than
CRISPR-Cas9
are
being
intensively
studied
field
editing.
CRISPR-Cas12
its
varied
representative
examples
genome
tools
superior
properties
terms
vivo
compared
Cas9.
Recently,
TnpB
Fanzor
OMEGA
(obligate
mobile
element
guided
activity)
were
identified
ancestor
on
basis
phylogenetic
analysis.
Notably,
compact
sizes
Cas12
endonucleases
allow
adeno-associated
virus
(AAV)
delivery;
hence,
they
set
challenge
Cas9
for
therapy.
This
review
is
focused
these
RNA-guided
endonucleases:
their
structure,
biochemistry,
off-target
effects,
applications
therapeutic
aBIOTECH,
Год журнала:
2024,
Номер
5(2), С. 225 - 230
Опубликована: Июнь 8, 2024
The
widely
used
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)/CRISPR-associated
nuclease
(Cas)
system
is
thought
to
have
evolved
from
IS200/IS605
transposons.
TnpB
proteins,
encoded
by
one
type
of
transposon,
are
considered
be
the
evolutionary
ancestors
Cas12
nucleases,
which
been
engineered
function
as
RNA-guided
DNA
endonucleases
for
genome
editing
in
bacteria
and
human
cells.
smaller
than
Cas
use
animal
systems,
but
feasibility
this
approach
plants
remained
unknown.
Here,
we
obtained
stably
transformed
genome-edited
mutants
rice
(
Gene-editing
technology
has
become
a
transformative
tool
for
the
precise
manipulation
of
biological
genomes
and
holds
great
significance
in
field
animal
disease-resistant
breeding.
Mastitis,
prevalent
disease
husbandry,
imposes
substantial
economic
burden
on
global
dairy
industry.
In
this
study,
regulatory
sequence
gene
editing
breeding
strategy
successful
creation
gene-edited
(GED)
goats
with
enhanced
mastitis
resistance
using
ISDra2-TnpB
system
as
model
is
proposed.
This
included
targeted
integration
an
innate
inflammatory
(IRS)
into
promoter
region
lysozyme
(LYZ)
gene.
Upon
Escherichia
Coli
(E.
coli)
mammary
gland
infection,
GED
exhibited
increased
LYZ
expression,
showing
robust
anti-mastitis
capabilities,
mitigating
PANoptosis
activation,
alleviating
blood-milk-barrier
(BMB)
damage.
Notably,
highly
expressed
only
E.
coli
infection.
study
marks
advent
animals
exogenous-free
expression
demonstrates
feasibility
gene-editing
proposed
study.
addition,
it
provides
novel
blueprint
developing
strains,
focusing
specificity
biosafety
while
providing
research
basis
widespread
application
system.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Авг. 16, 2024
Banana
(
Musa
spp.),
including
plantain,
is
one
of
the
major
staple
food
and
cash
crops
grown
in
over
140
countries
subtropics
tropics,
with
around
153
million
tons
annual
global
production,
feeding
about
400
people.
Despite
its
widespread
cultivation
adaptability
to
diverse
environments,
banana
production
faces
significant
challenges
from
pathogens
pests
that
often
coexist
within
agricultural
landscapes.
Recent
advancements
CRISPR/Cas-based
gene
editing
offer
transformative
solutions
enhance
resilience
productivity.
Researchers
at
IITA,
Kenya,
have
successfully
employed
confer
resistance
diseases
such
as
Xanthomonas
wilt
(BXW)
by
targeting
susceptibility
genes
streak
virus
(BSV)
disrupting
viral
sequences.
Other
breakthroughs
include
development
semi-dwarf
plants,
increased
β-carotene
content.
Additionally,
non-browning
been
developed
reduce
waste,
regulatory
approval
Philippines.
The
future
prospects
looks
promising
CRISPR-based
activation
(CRISPRa)
inhibition
(CRISPRi)
techniques
offering
potential
for
improved
disease
resistance.
Cas-CLOVER
system
provides
a
precise
alternative
CRISPR/Cas9,
demonstrating
success
generating
gene-edited
mutants.
Integration
precision
genetics
traditional
breeding,
adopting
transgene-free
strategies,
will
be
pivotal
harnessing
full
banana.
crop
holds
exciting
producing
thrives
across
agroecological
zones
offers
superior
nutritional
value,
ultimately
benefiting
farmers
consumers.
This
article
highlights
role
CRISPR/Cas
technology
advancing
resilience,
yield
quality,
implications
security.
