Cell & Bioscience, Год журнала: 2025, Номер 15(1)
Опубликована: Май 28, 2025
Язык: Английский
Nature Biotechnology, Год журнала: 2024, Номер unknown
Опубликована: Май 27, 2024
Abstract Long-term observation of subcellular dynamics in living organisms is limited by background fluorescence originating from tissue scattering or dense labeling. Existing confocal approaches face an inevitable tradeoff among parallelization, resolution and phototoxicity. Here we present scanning light-field microscopy (csLFM), which integrates axially elongated line-confocal illumination with the rolling shutter (sLFM). csLFM enables high-fidelity, high-speed, three-dimensional (3D) imaging at near-diffraction-limit both optical sectioning low By simultaneous 3D excitation detection, intensity can be reduced below 1 mW mm − 2 , 15-fold higher signal-to-background ratio over sLFM. We imaged 25,000 timeframes optically challenging environments different species, such as migrasome delivery mouse spleen, retractosome generation liver voltage Drosophila . Moreover, facilitates large-scale neural recording crosstalk, leading to high orientation selectivity visual stimuli, similar two-photon microscopy, aids understanding coding mechanisms.
Язык: Английский
Процитировано
19
Communications Biology, Год журнала: 2025, Номер 8(1)
Опубликована: Янв. 20, 2025
Almost all high-grade gliomas, particularly glioblastoma (GBM), are highly migratory and aggressive. Migrasomes organelles produced by cells capable of mediating intercellular communication. Thus, GBM may produce migrasomes during migration. However, it remains unclear whether can influence migration invasion. In this study, we observed the presence formation in cells. We found that expression levels key migrasome factor, tetraspanin 4 (TSPAN4), correlated positively with pathological grade poor prognosis based on databases clinical samples analysis. Subsequently, knocked down TSPAN4 cell invasion were significantly inhibited due to reduced migrasomes. further confirmed enriched extracellular matrix (ECM)-related proteins such as p21-activating kinase (PAK4) laminin alpha (LAMA4). Our experimental results suggest promote releasing into space. Overall, identified molecular mechanisms which they regulate them, providing potential targets for treating GBM. Observation (GBM) analysis migrasome-enriched revealed matrix-associated PAK4 LAMA4.
Язык: Английский
Процитировано
6
Nature Methods, Год журнала: 2025, Номер unknown
Опубликована: Май 12, 2025
Язык: Английский
Процитировано
3
Cell Death Discovery, Год журнала: 2023, Номер 9(1)
Опубликована: Окт. 18, 2023
Abstract Migrasome is a novel cellular organelle produced during cell migration, and its biogenesis depends on the migration process. It generated in variety of cells such as immune cells, metastatic tumor other special functional like podocytes developing organisms. plays important roles various fields especially information exchange between cells. The discovery migrasome, an supplement to extracellular vesicle system, provides new mechanisms targets for comprehending biological or pathological processes. In this article, we will review discovery, structure, distribution, detection, biogenesis, removal migrasomes mainly focus summarizing functions cell-to-cell communication, homeostatic maintenance, embryonic development multiple diseases. This also creates prospects possible research directions clinical applications future.
Язык: Английский
Процитировано
31
Nature Computational Science, Год журнала: 2023, Номер 3(12), С. 1067 - 1080
Опубликована: Дек. 11, 2023
Abstract Fluorescence imaging with high signal-to-noise ratios has become the foundation of accurate visualization and analysis biological phenomena. However, inevitable noise poses a formidable challenge to sensitivity. Here we provide spatial redundancy denoising transformer (SRDTrans) remove from fluorescence images in self-supervised manner. First, sampling strategy based on is proposed extract adjacent orthogonal training pairs, which eliminates dependence speed. Second, designed lightweight spatiotemporal architecture capture long-range dependencies high-resolution features at low computational cost. SRDTrans can restore high-frequency information without producing oversmoothed structures distorted traces. Finally, demonstrate state-of-the-art performance single-molecule localization microscopy two-photon volumetric calcium imaging. does not contain any assumptions about process sample, thus be easily extended various modalities applications.
Язык: Английский
Процитировано
27
Journal of Experimental & Clinical Cancer Research, Год журнала: 2024, Номер 43(1)
Опубликована: Июнь 6, 2024
Abstract Background The tetraspanin family plays a pivotal role in the genesis of migrasomes, and Tetraspanin CD151 is also implicated neovascularization within tumorous contexts. Nevertheless, research pertaining to involvement hepatocellular carcinoma (HCC) its association with migrasomes remains inadequate. Methods To investigate correlation between migrasome marker TSPAN4 liver cancer, we conducted database analysis using clinical data from HCC patients. Expression levels were assessed tissues correlated patient survival outcomes. In vitro experiments performed cell lines evaluate impact expression on formation cellular invasiveness. Cell altered utilized study generation invasion capabilities. Additionally, function was explored through aggregation assays phagocytosis studies. Subsequent VEGF level tissue chip further confirmed mediating angiogenesis signal transduction. Results Our revealed significant based samples. High closely associated poor outcomes Experimentally, decreased led reduced diminished capabilities, resulting attenuated vivo metastatic potential. Migrasomes demonstrated facilitate phagocytosis, thereby promoting Furthermore, VEGF-enriched signaling angiogenesis, accelerating progression. Conclusions summary, our findings support notion that elevated promotes formation, play invasiveness cancer cells, facilitating This finding implies generated by may constitute promising high-priority target for anti-angiogenic therapy HCC, offering crucial insights in-depth exploration renewed comprehension mechanism underlying metastasis.
Язык: Английский
Процитировано
18
Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(6)
Опубликована: Июнь 1, 2024
Abstract Migrasomes represent a recently uncovered category of extracellular microvesicles, spanning diameter range 500 to 3000 nm. They are emitted by migrating cells and harbour diverse array RNAs proteins. can be readily identified in bodily fluids like serum urine, rendering them valuable non‐invasive source for disease diagnosis through liquid biopsy. In this investigation, we introduce streamlined effective approach the capture quantitative assessment migrasomes, employing wheat germ agglutinin (WGA)‐coated magnetic beads flow cytometry (referred as WBFC). Subsequently, examined levels migrasomes urine kidney (KD) patients with podocyte injury healthy volunteers using WBFC. The outcomes unveiled substantial increase urinary podocyte‐derived migrasome concentrations among individuals KD compared counterparts. Notably, were found express an abundant quantity phospholipase A2 receptor (PLA2R) presence PLA2R proteins these holds promise serving natural antigen quantification autoantibodies against afflicted membranous nephropathy. Consequently, our study not only pioneers novel technique isolation but also underscores potential promising biomarker early injury.
Язык: Английский
Процитировано
14
Nature Machine Intelligence, Год журнала: 2024, Номер 6(6), С. 714 - 725
Опубликована: Июнь 24, 2024
Язык: Английский
Процитировано
12
Cell, Год журнала: 2024, Номер unknown
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
11
PhotoniX, Год журнала: 2024, Номер 5(1)
Опубликована: Янв. 5, 2024
Abstract Visualizing rapid biological dynamics like neuronal signaling and microvascular flow is crucial yet challenging due to photon noise motion artifacts. Here we present a deep learning framework for enhancing the spatiotemporal relations of optical microscopy data. Our approach leverages correlations mirrored perspectives from conjugated scan paths, training model suppress blur by restoring degraded spatial features. Quantitative validation on vibrational calcium imaging validates significant gains in correlation (2.2×), signal-to-noise ratio (9–12 dB), structural similarity (6.6×), tolerance compared raw We further apply diverse viv o experiments mouse cerebral hemodynamics zebrafish cardiac dynamics. This enables clear visualization nutrient (30 mm/s) microcirculation systolic diastolic processes heartbeat (2.7 cycle/s), as well cellular vascular structure cortex. Unlike techniques relying temporal correlations, inherent priors avoids motion-induced self-supervised strategy flexibly enhances live under photon-limited motion-prone regimes.
Язык: Английский
Процитировано
7