Rapid Communications in Mass Spectrometry,
Год журнала:
2025,
Номер
39(13)
Опубликована: Апрель 13, 2025
ABSTRACT
Single‐cell
metabolomics
is
an
emerging
and
powerful
technology
that
uncovers
intercellular
heterogeneity
reveals
microenvironmental
dynamics
in
both
physiological
pathological
conditions.
This
enables
detailed
observations
of
cellular
interactions,
providing
valuable
insights
into
processes
such
as
aging,
immune
responses,
disease
development.
Despite
significant
advances,
the
need
for
discussions
on
sampling
analytical
methods
single‐cell
continues
to
grow,
with
increasing
focus
selecting
most
suitable
techniques
diverse
research
objectives.
review
addresses
these
challenges
by
exploring
key
strategies
used
metabolomics.
We
three
main
approaches:
capture
isolation
specific
cell
types,
precise
aspiration
individual
cells,
situ
mass
spectrometry
imaging.
These
are
critically
assessed
highlight
achieving
accurate
metabolite
detection
at
level
across
applications.
Antioxidants,
Год журнала:
2025,
Номер
14(2), С. 201 - 201
Опубликована: Фев. 10, 2025
Oxidative
stress
is
a
common
event
involved
in
cancer
pathophysiology,
frequently
accompanied
by
unique
lipid
metabolic
reprogramming
phenomena.
caused
mainly
an
imbalance
between
the
production
of
reactive
oxygen
species
(ROS)
and
antioxidant
system
cells.
Emerging
evidence
has
reported
that
oxidative
regulates
expression
activity
metabolism-related
enzymes,
leading
to
alteration
cellular
metabolism;
this
involves
significant
increase
fatty
acid
synthesis
shift
way
which
lipids
are
taken
up
utilized.
The
dysregulation
metabolism
provides
abundant
intermediates
synthesize
biological
macromolecules
for
rapid
proliferation
cells;
moreover,
it
contributes
maintenance
intracellular
redox
homeostasis
producing
variety
reducing
agents.
Moreover,
derivatives
metabolites
play
critical
roles
signal
transduction
within
cells
tumor
microenvironment
evades
immune
destruction
facilitates
invasion
metastasis.
These
findings
suggest
close
relationship
during
malignant
progression
cancers.
This
review
focuses
on
crosstalk
reprogramming,
in-depth
insight
into
modulation
ROS
cancers
discusses
potential
strategies
targeting
therapy.
Signal Transduction and Targeted Therapy,
Год журнала:
2025,
Номер
10(1)
Опубликована: Март 7, 2025
Abstract
Macrophages
are
immune
cells
belonging
to
the
mononuclear
phagocyte
system.
They
play
crucial
roles
in
defense,
surveillance,
and
homeostasis.
This
review
systematically
discusses
types
of
hematopoietic
progenitors
that
give
rise
macrophages,
including
primitive
progenitors,
erythro-myeloid
stem
cells.
These
have
distinct
genetic
backgrounds
developmental
processes.
Accordingly,
macrophages
exhibit
complex
diverse
functions
body,
phagocytosis
clearance
cellular
debris,
antigen
presentation,
response,
regulation
inflammation
cytokine
production,
tissue
remodeling
repair,
multi-level
regulatory
signaling
pathways/crosstalk
involved
homeostasis
physiology.
Besides,
tumor-associated
a
key
component
TME,
exhibiting
both
anti-tumor
pro-tumor
properties.
Furthermore,
functional
status
is
closely
linked
development
various
diseases,
cancer,
autoimmune
disorders,
cardiovascular
disease,
neurodegenerative
metabolic
conditions,
trauma.
Targeting
has
emerged
as
promising
therapeutic
strategy
these
contexts.
Clinical
trials
macrophage-based
targeted
drugs,
immunotherapies,
nanoparticle-based
therapy
were
comprehensively
summarized.
Potential
challenges
future
directions
targeting
also
been
discussed.
Overall,
our
highlights
significance
this
versatile
cell
human
health
which
expected
inform
research
clinical
practice.
Seminars in Cancer Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 1, 2024
Elevated
lipid
metabolism
is
one
of
hallmarks
malignant
tumors.
Lipids
not
only
serve
as
essential
structural
components
biological
membranes
but
also
provide
energy
and
substrates
for
the
proliferation
cancer
cells
tumor
growth.
Cancer
meet
their
needs
by
coordinating
processes
absorption,
synthesis,
transport,
storage,
catabolism.
As
research
in
this
area
continues
to
deepen,
numerous
new
discoveries
have
emerged,
making
it
crucial
scientists
stay
informed
about
developments
metabolism.
In
review,
we
first
discuss
relevant
concepts
theories
or
assumptions
that
help
us
understand
-based
therapies.
We
then
systematically
summarize
latest
advancements
including
mechanisms,
novel
targets,
up-to-date
pre-clinical
clinical
investigations
anti-cancer
treatment
with
targeted
drugs.
Finally,
emphasize
emerging
directions
therapeutic
strategies,
future
prospective
challenges.
This
review
aims
insights
guidance
field
Glioblastoma
(GBM)
is
a
highly
aggressive
brain
tumor
characterized
by
its
ability
to
evade
the
immune
system,
hindering
efficacy
of
current
immunotherapies.
Recent
research
has
highlighted
important
role
immunosuppressive
macrophages
in
microenvironment
(TME)
driving
this
evasion.
In
study,
we
are
first
identify
THEMIS2
as
key
regulator
tumor-associated
macrophage
(TAM)-mediated
immunosuppression
GBM.
We
found
that
high
expression
associated
with
poor
patient
outcomes
and
increased
infiltration
cells,
particularly
macrophages.
Functional
analyses
revealed
THEMIS2's
critical
involvement
immune-related
pathways,
including
response
activation,
mononuclear
cell
differentiation,
positive
regulation
cytokine
production.
Additionally,
single-cell
RNA
sequencing
data
demonstrated
were
phagocytosis,
suppression,
enhanced
growth.
These
findings
suggest
could
serve
both
prognostic
marker
therapeutic
target
for
enhancing
anti-tumor
immunity
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 7, 2025
A
major
characteristic
of
ovarian
cancer
(OC)
is
its
unique
route
metastasis
via
ascites.
The
immune
microenvironment
in
ascites
remains
understudied,
leaving
the
mechanism
ascites-mediated
abdominal
obscure.
Here,
a
single-cell
transcriptomic
landscape
CD45+
cells
across
multiple
anatomical
sites
depicted,
including
primary
tumors,
metastatic
lesions,
and
ascites,
from
patients
diagnosed
with
high-grade
serous
carcinoma
(HGSOC).
novel
subset
perilipin
2
high
(PLIN2hi)
macrophages
are
identified
that
enriched
positively
correlated
OC
progression,
hence
being
designated
as
"ascites-associated
(AAMs)".
AAMs
lipid-loaded
overexpression
lipid
droplet
protein
PLIN2.
Overexpression
or
suppression
PLIN2
can
enhance
inhibit
tumor
cell
migration,
invasion,
vascular
permeability
vitro,
which
also
confirmed
vivo.
Mechanistically,
it
demonstrated
boosts
HIF1α/SPP1
signaling
macrophages,
thereby
exerting
pro-tumor
functions.
Finally,
PLIN2-targeting
liposome
designed
to
efficiently
suppress
production
metastasis.
Taken
together,
this
work
provides
comprehensive
characterization
cancer-promoting
function
lipid-rich
property
ascites-enriched
PLIN2hi
establishes
link
between
metabolism
hypoxia
within
context
microenvironment,
elucidates
pivotal
role
trans-coelomic
OC.
