Advances in Sampling and Analytical Techniques for Single‐Cell Metabolomics: Exploring Cellular Heterogeneity DOI

Xinxin Shen,

Fangyuan Zhang, Chunping Tang

et al.

Rapid Communications in Mass Spectrometry, Journal Year: 2025, Volume and Issue: 39(13)

Published: April 13, 2025

ABSTRACT Single‐cell metabolomics is an emerging and powerful technology that uncovers intercellular heterogeneity reveals microenvironmental dynamics in both physiological pathological conditions. This enables detailed observations of cellular interactions, providing valuable insights into processes such as aging, immune responses, disease development. Despite significant advances, the need for discussions on sampling analytical methods single‐cell continues to grow, with increasing focus selecting most suitable techniques diverse research objectives. review addresses these challenges by exploring key strategies used metabolomics. We three main approaches: capture isolation specific cell types, precise aspiration individual cells, situ mass spectrometry imaging. These are critically assessed highlight achieving accurate metabolite detection at level across applications.

Language: Английский

Energy metabolism in health and diseases DOI Creative Commons
Hui Liu, Shuo Wang, Jianhua Wang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: Feb. 18, 2025

Language: Английский

Citations

7
Oxidative Stress and Reprogramming of Lipid Metabolism in Cancers DOI Creative Commons
Siqi Li, Hang Yuan, Liang Li

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 201 - 201

Published: Feb. 10, 2025

Oxidative stress is a common event involved in cancer pathophysiology, frequently accompanied by unique lipid metabolic reprogramming phenomena. caused mainly an imbalance between the production of reactive oxygen species (ROS) and antioxidant system cells. Emerging evidence has reported that oxidative regulates expression activity metabolism-related enzymes, leading to alteration cellular metabolism; this involves significant increase fatty acid synthesis shift way which lipids are taken up utilized. The dysregulation metabolism provides abundant intermediates synthesize biological macromolecules for rapid proliferation cells; moreover, it contributes maintenance intracellular redox homeostasis producing variety reducing agents. Moreover, derivatives metabolites play critical roles signal transduction within cells tumor microenvironment evades immune destruction facilitates invasion metastasis. These findings suggest close relationship during malignant progression cancers. This review focuses on crosstalk reprogramming, in-depth insight into modulation ROS cancers discusses potential strategies targeting therapy.

Language: Английский

Citations

3
Tissue macrophages: origin, heterogenity, biological functions, diseases and therapeutic targets DOI Creative Commons

Fan Guan,

Ruixuan Wang,

Zhenjie Yi

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2025, Volume and Issue: 10(1)

Published: March 7, 2025

Abstract Macrophages are immune cells belonging to the mononuclear phagocyte system. They play crucial roles in defense, surveillance, and homeostasis. This review systematically discusses types of hematopoietic progenitors that give rise macrophages, including primitive progenitors, erythro-myeloid stem cells. These have distinct genetic backgrounds developmental processes. Accordingly, macrophages exhibit complex diverse functions body, phagocytosis clearance cellular debris, antigen presentation, response, regulation inflammation cytokine production, tissue remodeling repair, multi-level regulatory signaling pathways/crosstalk involved homeostasis physiology. Besides, tumor-associated a key component TME, exhibiting both anti-tumor pro-tumor properties. Furthermore, functional status is closely linked development various diseases, cancer, autoimmune disorders, cardiovascular disease, neurodegenerative metabolic conditions, trauma. Targeting has emerged as promising therapeutic strategy these contexts. Clinical trials macrophage-based targeted drugs, immunotherapies, nanoparticle-based therapy were comprehensively summarized. Potential challenges future directions targeting also been discussed. Overall, our highlights significance this versatile cell human health which expected inform research clinical practice.

Language: Английский

Citations

3
Embracing diversity: macrophage complexity in cancer DOI

Jan Hochstadt,

Sabino Pacheco, María Casanova-Acebes

et al.

Trends in cancer, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
The Impact of Metabolic Rewiring in Glioblastoma: The Immune Landscape and Therapeutic Strategies DOI Open Access

Yuganthini Vijayanathan,

Ivy A. W. Ho

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 669 - 669

Published: Jan. 14, 2025

Glioblastoma (GBM) is an aggressive brain tumor characterized by extensive metabolic reprogramming that drives growth and therapeutic resistance. Key pathways, including glycolysis, lactate production, lipid metabolism, are upregulated to sustain survival in the hypoxic nutrient-deprived microenvironment (TME), while glutamine tryptophan metabolism further contribute phenotype of GBM. These alterations impair immune cell function, leading exhaustion stress CD8+ CD4+ T cells favoring immunosuppressive populations such as regulatory (Tregs) M2-like macrophages. Recent studies emphasize role slow-cycling GBM (SCCs), lipid-laden macrophages, tumor-associated astrocytes (TAAs) reshaping GBM’s landscape reinforcing evasion. Genetic mutations, Isocitrate Dehydrogenase (IDH) Epidermal Growth Factor Receptor (EGFR) amplification, Phosphotase Tensin Homolog (PTEN) loss, drive offer potential targets for therapy. Understanding relationship between suppression critical overcoming This review focuses on rewiring GBM, its impact microenvironment, combining targeting with immunotherapy improve clinical outcomes patients.

Language: Английский

Citations

1
Theoretical Framework and Emerging Challenges of Lipid Metabolism in Cancer DOI Creative Commons
Qiuying Gu, Yuan Wang, Ping Yi

et al.

Seminars in Cancer Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Elevated lipid metabolism is one of hallmarks malignant tumors. Lipids not only serve as essential structural components biological membranes but also provide energy and substrates for the proliferation cancer cells tumor growth. Cancer meet their needs by coordinating processes absorption, synthesis, transport, storage, catabolism. As research in this area continues to deepen, numerous new discoveries have emerged, making it crucial scientists stay informed about developments metabolism. In review, we first discuss relevant concepts theories or assumptions that help us understand -based therapies. We then systematically summarize latest advancements including mechanisms, novel targets, up-to-date pre-clinical clinical investigations anti-cancer treatment with targeted drugs. Finally, emphasize emerging directions therapeutic strategies, future prospective challenges. This review aims insights guidance field

Language: Английский

Citations

5
Comprehensive Analysis Identifies THEMIS2 as a Potential Prognostic and Immunological Biomarker in Glioblastoma DOI Creative Commons
Jianan Chen, Qiong Wu, Anders Berglund

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(2), P. 66 - 66

Published: Jan. 7, 2025

Glioblastoma (GBM) is a highly aggressive brain tumor characterized by its ability to evade the immune system, hindering efficacy of current immunotherapies. Recent research has highlighted important role immunosuppressive macrophages in microenvironment (TME) driving this evasion. In study, we are first identify THEMIS2 as key regulator tumor-associated macrophage (TAM)-mediated immunosuppression GBM. We found that high expression associated with poor patient outcomes and increased infiltration cells, particularly macrophages. Functional analyses revealed THEMIS2's critical involvement immune-related pathways, including response activation, mononuclear cell differentiation, positive regulation cytokine production. Additionally, single-cell RNA sequencing data demonstrated were phagocytosis, suppression, enhanced growth. These findings suggest could serve both prognostic marker therapeutic target for enhancing anti-tumor immunity

Language: Английский

Citations

0
Spatial omics shed light on the tumour organisation of glioblastoma DOI Creative Commons
James R. Whittle, Jurgen Kriel, Ebenezer O. Fatunla

et al.

Seminars in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 167, P. 1 - 9

Published: Jan. 8, 2025

Language: Английский

Citations

0
Metabolic cross-talk between glioblastoma and glioblastoma-associated microglia/macrophages: From basic insights to therapeutic strategies DOI
Yuan Gao, Mengxia Zhang, Guihua Wang

et al.

Critical Reviews in Oncology/Hematology, Journal Year: 2025, Volume and Issue: 208, P. 104649 - 104649

Published: Feb. 6, 2025

Language: Английский

Citations

0
PLIN2 Promotes Lipid Accumulation in Ascites‐Associated Macrophages and Ovarian Cancer Progression by HIF1α/SPP1 Signaling DOI Creative Commons
Hui Luo,

Xiaoling She,

Yubo Zhang

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

A major characteristic of ovarian cancer (OC) is its unique route metastasis via ascites. The immune microenvironment in ascites remains understudied, leaving the mechanism ascites-mediated abdominal obscure. Here, a single-cell transcriptomic landscape CD45+ cells across multiple anatomical sites depicted, including primary tumors, metastatic lesions, and ascites, from patients diagnosed with high-grade serous carcinoma (HGSOC). novel subset perilipin 2 high (PLIN2hi) macrophages are identified that enriched positively correlated OC progression, hence being designated as "ascites-associated (AAMs)". AAMs lipid-loaded overexpression lipid droplet protein PLIN2. Overexpression or suppression PLIN2 can enhance inhibit tumor cell migration, invasion, vascular permeability vitro, which also confirmed vivo. Mechanistically, it demonstrated boosts HIF1α/SPP1 signaling macrophages, thereby exerting pro-tumor functions. Finally, PLIN2-targeting liposome designed to efficiently suppress production metastasis. Taken together, this work provides comprehensive characterization cancer-promoting function lipid-rich property ascites-enriched PLIN2hi establishes link between metabolism hypoxia within context microenvironment, elucidates pivotal role trans-coelomic OC.

Language: Английский

Citations

0