Algal chloroplast pyrenoids: Evidence for convergent evolution
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(14)
Опубликована: Март 21, 2024
Язык: Английский
Harnessing microalgae: Innovations for achieving UN Sustainable Development Goals and climate resilience
Journal of Water Process Engineering,
Год журнала:
2024,
Номер
68, С. 106506 - 106506
Опубликована: Ноя. 18, 2024
Язык: Английский
DiatOmicBase: a versatile gene‐centered platform for mining functional omics data in diatom research
The Plant Journal,
Год журнала:
2025,
Номер
121(6)
Опубликована: Март 1, 2025
SUMMARY
Diatoms
are
prominent
microalgae
found
in
all
aquatic
environments.
Over
the
last
20
years,
thanks
to
availability
of
genomic
and
genetic
resources,
diatom
species
such
as
Phaeodactylum
tricornutum
Thalassiosira
pseudonana
have
emerged
valuable
experimental
model
systems
for
exploring
topics
ranging
from
evolution
cell
biology,
(eco)physiology,
biotechnology.
Since
first
genome
sequencing
projects
initiated
more
than
years
ago,
numerous
genome‐enabled
datasets
been
generated,
based
on
RNA‐Seq
proteomics
experiments,
epigenomes,
ecotype
variant
analysis.
Unfortunately,
these
generated
by
various
laboratories,
often
disparate
formats
challenging
access
analyze.
Here
we
present
DiatOmicBase,
a
portal
gathering
comprehensive
omics
resources
P.
T.
facilitate
exploration
dispersed
public
design
new
experiments
prior‐art.
DiatOmicBase
provides
gene
annotations,
transcriptomic
profiles
browser
with
variants,
histone
methylation
marks,
transposable
elements,
non‐coding
RNAs,
read
densities
experiments.
We
developed
semi‐automatically
updated
module
explore
both
publicly
available
users'
private
datasets.
Using
gene‐level
expression
data,
users
can
perform
exploratory
data
analysis,
differential
expression,
pathway
biclustering,
co‐expression
network
Users
create
heatmaps
visualize
pre‐computed
comparisons
selected
subsets.
Automatic
other
bioinformatic
tools
comparative
functional
genomics
is
also
provided.
Focusing
currently
centralized
,
showcase
several
examples
how
strengthens
molecular
research
diatoms,
making
organisms
accessible
broad
community.
Язык: Английский
Multiphase CO2-dependent photosynthesis in marine diatoms
Frontiers in Photobiology,
Год журнала:
2025,
Номер
3
Опубликована: Апрель 2, 2025
Diatoms,
one
of
the
most
ubiquitous
phytoplankton
in
oceans,
have
evolved
a
pyrenoid-based
CO
2
-concentrating
mechanism
(CCM)
to
utilize
limited
seawater
for
photosynthesis.
Recent
proteomics
analyses
and
molecular
biological
tools
deepened
our
understanding
mechanisms
involved
diatom
chloroplast
architecture
CCM.
Here,
we
provide
an
update
knowledge
processes
high
affinity
photosynthesis
dissolved
inorganic
carbon
(DIC)
diatoms.
Based
on
phenotype
genome-edited
mutants,
propose
model
CCM
composed
four
phases
-dependent
at
(I)
less
than
0.1
mM,
(II)
0.1–2
(III)
2–10
(IV)
more
10
mM
DIC
concentrations,
which
rate-determining
steps
are
capture
unfixed
stroma
Phases
I
II,
evolution
pyrenoid-penetrating
thylakoid
lumen
Phase
III,
transport
IV.
Under
natural
containing
mainly
form
HCO
3
−
,
marine
diatoms
is
likely
primarily
shifting
II
when
available
limited.
Язык: Английский
Genetic engineering in diatoms: advances and prospects
The Plant Journal,
Год журнала:
2025,
Номер
121(6)
Опубликована: Март 1, 2025
SUMMARY
Diatoms
are
among
the
most
diverse
and
ecologically
significant
groups
of
photosynthetic
microalgae,
contributing
over
20%
global
primary
productivity.
Their
ecological
significance,
unique
biology,
genetic
tractability
make
them
ideal
targets
for
genomic
engineering
metabolic
reprogramming.
Over
past
few
decades,
numerous
methods
have
been
developed
applied
to
these
organisms
better
understand
function
individual
genes
how
they
underpin
diatom
metabolism.
Additionally,
ability
diatoms
synthesize
high‐value
metabolites
elaborate
mineral
structures
offers
potential
applications
in
biotechnology,
including
synthesis
novel
pharmaceuticals,
nutraceuticals,
biomaterials.
This
review
discusses
latest
developments
provides
prospects
not
only
promote
use
fields
biotechnology
but
also
deepen
our
understanding
their
role
natural
ecosystems.
Язык: Английский
Engineering microbial cell factories by multiplexed spatiotemporal control of cellular metabolism: Advances, challenges, and future perspectives
Biotechnology Advances,
Год журнала:
2024,
Номер
79, С. 108497 - 108497
Опубликована: Дек. 5, 2024
Язык: Английский
From algae to plants: understanding pyrenoid-based CO2-concentrating mechanisms
Trends in Biochemical Sciences,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 1, 2024
Язык: Английский
Spotlight on “A protein blueprint of the diatom CO 2 -fixing organelle” by Nam et al. (2024) and “Diatom pyrenoids are encased in a protein shell that enables efficient CO 2 fixation” by Shimakawa et al. (2024)
Journal of Mitochondria Plastids and Endosymbiosis,
Год журнала:
2024,
Номер
3(1)
Опубликована: Дек. 24, 2024
Recent
studies
by
Nam
et
al.
and
Shimakawa
published
in
Cell
(2024)
have
elucidated
the
protein
composition
of
diatom
pyrenoids
constructing
protein–protein
interaction
networks
for
centric
Thalassiosira
pseudonana
using
vivo
photo-crosslinking
with
Rubisco
pennate
Phaeodactylum
tricornutum.
These
investigations
identified
a
family
proteins
that
form
pyrenoid
sheath,
termed
"Shell"
proteins.
Knockout
experiments
revealed
absence
or
malformation
these
sheaths
leads
to
altered
structures
impaired
growth
under
atmospheric
CO2
conditions.
The
research
highlights
are
enclosed
lattice-like
proteinaceous
sheaths,
as
demonstrated
through
multiscale
imaging
techniques.
findings
provide
insights
into
convergent
evolution
across
various
algal
lineages
suggest
potential
strategies
engineering
crop
plants
enhance
agricultural
yields.
Язык: Английский