Hepatitis C Virus NS5A Activates Mitophagy Through Cargo Receptor and Phagophore Formation DOI Creative Commons

Yuan-Chao Hsiao,

Chih-Wei Chang,

Chau‐Ting Yeh

и другие.

Pathogens, Год журнала: 2024, Номер 13(12), С. 1139 - 1139

Опубликована: Дек. 23, 2024

Chronic HCV infection is a risk factor for end-stage liver disease, leading to major burden on public health. Mitophagy specific form of selective autophagy that eliminates mitochondria maintain mitochondrial integrity. NS5A multifunctional protein regulates the life cycle and may induce host mitophagy. However, molecular mechanism by which activates mitophagy remains largely unknown. Here, first time, we delineate dynamic process NS5A-activated PINK1/Parkin-dependent By performing live-cell imaging CLEM analyses NS5A-expressing cells, demonstrate degradation within autophagic vacuoles, dependent Parkin ubiquitin translocation onto PINK1 stabilization. In addition, cargo receptors mitophagy, NDP52 OPTN, are recruited required NS5A-induced Moreover, ATG5 DFCP1, function in autophagosome closure phagophore formation, translocated near Furthermore, autophagy-initiating proteins, including ATG14 ULK1, NS5A-triggered Together, these findings through recognition nascent formation phagophores close mitochondria.

Язык: Английский

Polystyrene nanoplastics induce lipophagy via the AMPK/ULK1 pathway and block lipophagic flux leading to lipid accumulation in hepatocytes DOI

Zhuying Fan,

Yukang Zhang,

Yuting Fang

и другие.

Journal of Hazardous Materials, Год журнала: 2024, Номер 476, С. 134878 - 134878

Опубликована: Июнь 12, 2024

Язык: Английский

Процитировано

6

Quercetin ameliorates oxidative stress-induced apoptosis of granulosa cells in dairy cow follicular cysts by activating autophagy via the SIRT1/ROS/AMPK signaling pathway DOI Creative Commons
Hongwei Duan, Fang Wang, Ke Wang

и другие.

Journal of Animal Science and Biotechnology/Journal of animal science and biotechnology, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 5, 2024

Язык: Английский

Процитировано

5

The Interplay between Salmonella and Host: Mechanisms and Strategies for Bacterial Survival DOI Creative Commons
Hongyu Zhao, Xinyue Zhang,

Ningning Zhang

и другие.

Cell Insight, Год журнала: 2025, Номер 4(2), С. 100237 - 100237

Опубликована: Фев. 13, 2025

Salmonella, an intracellular pathogen, infects both humans and animals, causing diverse diseases such as gastroenteritis enteric fever. The Salmonella type III secretion system (T3SS), encoded within its pathogenicity islands (SPIs), is critical for bacterial virulence by directly delivering multiple effectors into eukaryotic host cells. utilizes these to facilitate survival replication the through modulating cytoskeletal dynamics, inflammatory responses, biogenesis of Salmonella-containing vacuole (SCV), cell survival. Moreover, also interfere with immune responses via inhibiting innate immunity or antigen presentation. In this review, we summarize current progress in strategies employed molecular mechanisms underlying interactions host. Understanding interplay between can enhance our knowledge bacterium's pathogenic processes provide new insights how it manipulates cellular physiological activities ensure

Язык: Английский

Процитировано

0

Hypomyelination Leukodystrophy 16 (HLD16)-Associated Mutation p.Asp252Asn of TMEM106B Blunts Cell Morphological Differentiation DOI Creative Commons

Sui Sawaguchi,

Miki Ishida, Yuki Miyamoto

и другие.

Current Issues in Molecular Biology, Год журнала: 2024, Номер 46(8), С. 8088 - 8103

Опубликована: Июль 27, 2024

Transmembrane protein 106B (TMEM106B), which is a type II transmembrane protein, believed to be involved in intracellular dynamics and morphogenesis the lysosome. TMEM106B known risk factor for frontotemporal lobar degeneration has been recently identified as receptor needed entry of SARS-CoV-2, independently angiotensin-converting enzyme 2 (ACE2). A missense mutation, p.Asp252Asn, associated with hypomyelinating leukodystrophy 16 (HLD16), an oligodendroglial cell-related white matter disorder causing thin myelin sheaths or deficiency central nervous system (CNS). However, it remains elucidated how mutated affects cells. Here, we show that mutant fails exhibit lysosome distribution FBD-102b cell line, precursor line undergoing differentiation. In contrast, wild-type was indeed localized Cells harboring differentiated into ones widespread membranes, whereas cells failed differentiate. It note output signaling through lysosome-resident mechanistic target rapamycin (mTOR) greatly decreased TMEM106B. Furthermore, treatment hesperetin, citrus flavonoid activator mTOR signaling, restored molecular cellular phenotypes induced by protein. These findings suggest potential pathological mechanisms underlying HLD16 their amelioration.

Язык: Английский

Процитировано

0

Autophagy and its role in gastrointestinal diseases DOI

Bo‐Zong Shao,

Wengang Zhang, Zhenyu Liu

и другие.

World Journal of Gastroenterology, Год журнала: 2024, Номер 30(36), С. 4014 - 4020

Опубликована: Сен. 20, 2024

Gastrointestinal disorders encompass a spectrum of conditions affecting various organs within the digestive system, such as esophagus, stomach, colon, rectum, pancreas, liver, small intestine, and bile ducts. The role autophagy in etiology progression gastrointestinal diseases has garnered significant attention. This paper seeks to evaluate impact mechanisms by synthesizing recent research findings. Specifically, we delve into inflammation-related conditions, including ul-cerative colitis, Crohn’s disease, pancreatitis, well cancers esophageal, gastric, colorectal cancers. Additionally, provide commentary on publication Chang et al World Journal Gastroenterology . Our objective is offer fresh perspectives therapeutic approaches for these ailments. review aims new strategies critically analyzing relevant publications. As discussed, complex and, at times, contentious. To harness full potential treating more in-depth imperative.

Язык: Английский

Процитировано

0

Functional Role of Hepatitis C Virus NS5A in the Regulation of Autophagy DOI Creative Commons
Po‐Yuan Ke,

Chau‐Ting Yeh

Pathogens, Год журнала: 2024, Номер 13(11), С. 980 - 980

Опубликована: Ноя. 8, 2024

Many types of RNA viruses, including the hepatitis C virus (HCV), activate autophagy in infected cells to promote viral growth and counteract host defense response. Autophagy acts as a catabolic pathway which unnecessary materials are removed via lysosome, thus maintaining cellular homeostasis. The HCV non-structural 5A (NS5A) protein is phosphoprotein required for replication, virion assembly, determination interferon (IFN) sensitivity. Recently, increasing evidence has shown that NS5A can induce mitochondrial turnover degradation hepatocyte nuclear factor 1 alpha (HNF-1α) diacylglycerol acyltransferase (DGAT1). In this review, we summarize recent progress understanding detailed mechanism by triggers autophagy, outline physiological significance balance between host-virus interactions.

Язык: Английский

Процитировано

0

Hepatitis C Virus NS5A Activates Mitophagy Through Cargo Receptor and Phagophore Formation DOI Creative Commons

Yuan-Chao Hsiao,

Chih-Wei Chang,

Chau‐Ting Yeh

и другие.

Pathogens, Год журнала: 2024, Номер 13(12), С. 1139 - 1139

Опубликована: Дек. 23, 2024

Chronic HCV infection is a risk factor for end-stage liver disease, leading to major burden on public health. Mitophagy specific form of selective autophagy that eliminates mitochondria maintain mitochondrial integrity. NS5A multifunctional protein regulates the life cycle and may induce host mitophagy. However, molecular mechanism by which activates mitophagy remains largely unknown. Here, first time, we delineate dynamic process NS5A-activated PINK1/Parkin-dependent By performing live-cell imaging CLEM analyses NS5A-expressing cells, demonstrate degradation within autophagic vacuoles, dependent Parkin ubiquitin translocation onto PINK1 stabilization. In addition, cargo receptors mitophagy, NDP52 OPTN, are recruited required NS5A-induced Moreover, ATG5 DFCP1, function in autophagosome closure phagophore formation, translocated near Furthermore, autophagy-initiating proteins, including ATG14 ULK1, NS5A-triggered Together, these findings through recognition nascent formation phagophores close mitochondria.

Язык: Английский

Процитировано

0