Visual and motor signatures of locomotion dynamically shape a population code for feature detection in Drosophila

eLife, Год журнала: 2022, Номер 11

Опубликована: Окт. 27, 2022

Natural vision is dynamic: as an animal moves, its visual input changes dramatically. How can the system reliably extract local features from dominated by self-generated signals? In Drosophila , diverse are represented a group of projection neurons with distinct tuning properties. Here, we describe connectome-based volumetric imaging strategy to measure visually evoked neural activity across this population. We show that jointly population, and shared gain factor improves trial-to-trial coding fidelity. A subset these neurons, tuned small objects, modulated two independent signals associated self-movement, motor-related signal, motion signal rotation animal. These inputs adjust sensitivity feature detectors locomotor cycle, selectively reducing their during saccades restoring it intersaccadic intervals. This work reveals for reliable detection locomotion.

Язык: Английский

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Numerical discrimination in Drosophila melanogaster

Cell Reports, Год журнала: 2023, Номер 42(7), С. 112772 - 112772

Опубликована: Июль 1, 2023

Sensitivity to numbers is a crucial cognitive ability. The lack of experimental models amenable systematic genetic and neural manipulation has precluded discovering circuits required for numerical cognition. Here, we demonstrate that Drosophila flies spontaneously prefer sets containing larger objects. This preference determined by the ratio between two quantities tested, characteristic signature cognition across species. Individual maintained their choice over consecutive days. Using visual conditioning paradigm, found are capable associating sucrose with can be trained reverse spontaneous large quantities. Finally, show silencing lobula columnar neurons (LC11) reduces more objects, thus identifying neuronal substrate in invertebrates. discovery paves way analysis behavioral mechanisms underlying evolutionary conserved sensitivity numerosity.

Язык: Английский

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Fractal video compression for IOT-based smart cities applications using motion vector estimation

Measurement Sensors, Год журнала: 2023, Номер 26, С. 100698 - 100698

Опубликована: Фев. 20, 2023

Recently, Internet of the things (IoTs) devices have been used highly in surveillance applications smart environments, cities, and buildings. Most data recorded by IoT is form video needs to be transmitted admin or server streaming technology. Fractal compression uses a self-similarity concept for compression, meaning fractal image contains itself that explained denoted change. Computational cost more coding; different methods developed reduce this computational cost. The proposed work's main objective implement approach combining Diamond-search-pattern- block-matching motion estimation algorithm hash-based encoding time. It improves both delay security block matching system measures based on evaluation parameters: Peak signal-to-noise ratio (PSNR), time, decoding Mean squared error (MSE), Compression Smart City Applications. Compared other algorithms, IoTs streamed efficiently city analyse abnormalities.

Язык: Английский

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Columnar neurons support saccadic bar tracking in Drosophila

eLife, Год журнала: 2023, Номер 12

Опубликована: Апрель 4, 2023

Tracking visual objects while maintaining stable gaze is complicated by the different computational requirements for figure-ground discrimination, and distinct behaviors that these computations coordinate. Drosophila melanogaster uses smooth optomotor head body movements to stabilize gaze, impulsive saccades pursue elongated vertical bars. Directionally selective motion detectors T4 T5 cells provide inputs large-field neurons in lobula plate, which control stabilization behavior. Here, we hypothesized an anatomically parallel pathway represented T3 cells, lobula, drives bar tracking saccades. We combined physiological behavioral experiments show respond omnidirectionally same stimuli elicit saccades, silencing reduced frequency of optogenetic manipulation acted on saccade rate a push–pull manner. Manipulating did not affect responses motion. Our results neural pathways coordinate saccadic behavior during flight.

Язык: Английский

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Neural Circuit Mechanisms Involved in Animals’ Detection of and Response to Visual Threats

Neuroscience Bulletin, Год журнала: 2023, Номер 39(6), С. 994 - 1008

Опубликована: Янв. 25, 2023

Язык: Английский

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NeuroMechFly 2.0, a framework for simulating embodied sensorimotor control in adultDrosophila

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 18, 2023

Abstract Discovering principles underlying the control of animal behavior requires a tight dialogue between experiments and neuromechanical models. Until now, such models, including NeuroMechFly for adult fly, Drosophila melanogaster , have primarily been used to investigate motor control. Far less studied with realistic body models is how brain systems work together perform hierarchical sensorimotor Here we present v2, framework that expands modeling by enabling visual olfactory sensing, ascending feedback, complex terrains can be navigated using leg adhesion. We illustrate its capabilities first constructing biologically inspired locomotor controllers use feedback path integration head stabilization. Then, add sensing this controller train it reinforcement learning multimodal navigation task in closed loop. Finally, more biorealistic two ways: our model navigates odor plume taxis strategy, uses connectome-constrained system network follow another simulated fly. With framework, accelerate discovery explanatory nervous develop machine learning-based autonomous artificial agents robots.

Язык: Английский

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Morphological and functional convergence of visual projection neurons from diverse neurogenic origins in Drosophila

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 15, 2025

The Drosophila visual system is a powerful model to study the development of neural circuits. Lobula columnar neurons-LCNs are output neurons that encode features relevant natural behavior. There ~20 classes LCNs forming non-overlapping synaptic optic glomeruli in brain. To address their origin, we used single-cell mRNA sequencing define transcriptome LCN subtypes and identified lines expressed throughout development. We show originate from stem cells four distinct brain regions exhibiting different modes neurogenesis, including ventral dorsal tips outer proliferation center, superficial inner center central this convergence similar illustrates complexity generating neuronal diversity, likely reflects evolutionary origin each subtype detects specific feature might influence behaviors species. Here authors developmental origins Columnar Neurons brain, tracing them regions. highlights diversity.

Язык: Английский

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Genetically-encoded markers for confocal visualization of single dense core vesicles

Communications Biology, Год журнала: 2025, Номер 8(1)

Опубликована: Март 7, 2025

Neuronal dense core vesicles (DCVs) store and release a diverse array of neuromodulators, trophic factors, bioamines. The analysis single DCVs has largely been possible only using electron microscopy, which makes understanding cargo segregation DCV heterogeneity difficult. To address these limitations, we develop genetically encoded markers for that can be used in combination with standard immunohistochemistry expansion microscopy to enable single-vesicle resolution confocal Drosophila.

Язык: Английский

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Social state alters vision using three circuit mechanisms in Drosophila

Nature, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 20, 2024

Abstract Animals are often bombarded with visual information and must prioritize specific features based on their current needs. The neuronal circuits that detect relay have been well studied 1–8 . Much less is known about how an animal adjusts its attention as goals or environmental conditions change. During social behaviours, flies need to focus nearby 9–11 Here we study the flow of altered when female Drosophila enter aggressive state. From connectome, identify three state-dependent circuit motifs poised modify response fly-sized objects: convergence excitatory inputs from neurons conveying select internal state; dendritic disinhibition feature detectors; a switch toggles between two detectors. Using cell-type-specific genetic tools, together behavioural neurophysiological analyses, show each these used during aggression. We reveal this same operate in male courtship pursuit, suggesting disparate behaviours may share mechanisms. Our provides compelling example using connectome infer mechanisms underlie dynamic processing sensory signals.

Язык: Английский

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Serotonergic modulation of visual neurons in Drosophila melanogaster

PLoS Genetics, Год журнала: 2020, Номер 16(8), С. e1009003 - e1009003

Опубликована: Авг. 31, 2020

Sensory systems rely on neuromodulators, such as serotonin, to provide flexibility for information processing stimuli vary, light intensity throughout the day. Serotonergic neurons broadly innervate optic ganglia of Drosophila melanogaster, a widely used model studying vision. It remains unclear whether serotonin modulates physiology interneurons in ganglia. To address this question, we first mapped expression patterns receptors visual system, focusing subset cells with processes ganglion, lamina. Serotonin receptor was found several types columnar lamina including 5-HT2B monopolar cell L2, required spatiotemporal luminance contrast, and both 5-HT1A 5-HT1B T1 cells, whose function is unknown. Subcellular mapping GFP-tagged constructs indicated that these localize layer M2 medulla, proximal serotonergic boutons, suggesting medulla neuropil primary site regulation neurons. Exogenous increased basal intracellular calcium L2 terminals modestly decreased duration visually induced transients following repeated dark flashes, but otherwise did not alter transients. Flies without functional failed show an increase response serotonin. mutants also change amplitude their flashes other transient parameters were relatively unaffected. While detect L1 neurons, they, like underwent serotonin-induced changes calcium, presumably via interactions cells. These data demonstrate involved early Drosophila.

Язык: Английский

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