Multisensory learning binds neurons into a cross-modal memory engram

Nature, Год журнала: 2023, Номер 617(7962), С. 777 - 784

Опубликована: Апрель 26, 2023

Associating multiple sensory cues with objects and experience is a fundamental brain process that improves object recognition memory performance. However, neural mechanisms bind features during learning augment expression are unknown. Here we demonstrate multisensory appetitive aversive in Drosophila. Combining colours odours improved performance, even when each modality was tested alone. Temporal control of neuronal function revealed visually selective mushroom body Kenyon cells (KCs) to be required for enhancement both visual olfactory after training. Voltage imaging head-fixed flies showed binds activity between streams modality-specific KCs so unimodal input generates multimodal response. Binding occurs regions the KC axons, which receive valence-relevant dopaminergic reinforcement, propagated downstream. Dopamine locally releases GABAergic inhibition permit specific microcircuits within KC-spanning serotonergic neurons as an excitatory bridge previously 'modality-selective' streams. Cross-modal binding thereby expands representing engram into those other. This broadening performance permits single feature retrieve experience.

Язык: Английский

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Internal state configures olfactory behavior and early sensory processing in Drosophila larvae

Science Advances, Год журнала: 2021, Номер 7(1)

Опубликована: Янв. 1, 2021

The first olfactory processing center in the larval Drosophila brain uses information about feeding state to shape behavior.

Язык: Английский

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Neuronal ageing is promoted by the decay of the microtubule cytoskeleton

PLoS Biology, Год журнала: 2024, Номер 22(3), С. e3002504 - e3002504

Опубликована: Март 13, 2024

Natural ageing is accompanied by a decline in motor, sensory, and cognitive functions, all impacting quality of life. Ageing also the predominant risk factor for many neurodegenerative diseases, including Parkinson’s disease Alzheimer’s disease. We need to therefore gain better understanding cellular physiological processes underlying age-related neuronal decay. However, gaining this slow process due large amount time required age mammalian or vertebrate animal models. Here, we introduce new model within Drosophila brain, which report classical hallmarks previously observed primate brain. These include axonal swellings, cytoskeletal decay, reduction calibre, morphological changes arising at synaptic terminals. In fly these begin occur few weeks, ideal study mechanisms ageing. discovered that decay microtubule (MT) cytoskeleton precedes onset other hallmarks. showed MT-binding factors Tau, EB1, Shot/MACF1, are necessary MT maintenance axons synapses, their functional loss during triggers bundle followed Furthermore, genetic manipulations improve networks slowed down confer aged specimens ability outperform age-matched controls. Our work suggests key lesion site neurons offers promising target advanced age.

Язык: Английский

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Constitutive and conditional epitope-tagging of endogenous G protein coupled receptors inDrosophila

Journal of Neuroscience, Год журнала: 2024, Номер 44(33), С. e2377232024 - e2377232024

Опубликована: Июнь 27, 2024

To visualize the cellular and subcellular localization of neuromodulatory G-protein–coupled receptors in Drosophila , we implement a molecular strategy recently used to add epitope tags ionotropic at their endogenous loci. Leveraging evolutionary conservation identify sites more likely permit insertion tag, generated constitutive conditional tagged alleles for 5-HT1A 5-HT2A 5-HT2B Oct β 1R 2R two isoforms OAMB mGluR . The allow restricted expression receptor specific cell types, an option not available any previous reagents label these proteins. We show patterns female brains that localize mushroom bodies (MBs) central complex, respectively, as predicted by roles sleep. By contrast, unexpected enrichment Octβ1R complex nerve terminals lobular columnar cells visual system suggest new hypotheses about functions sites. Using additional allele serotonin transporter, marker serotonergic tracts, demonstrate diverse spatial relationships between postsynaptic 5-HT presynaptic neurons, consistent with importance both synaptic volume transmission. Finally, use it localizes distinct within MBs Kenyon autoreceptor.

Язык: Английский

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Mutation of the Drosophila melanogaster serotonin transporter dSERT impacts sleep, courtship, and feeding behaviors

PLoS Genetics, Год журнала: 2022, Номер 18(11), С. e1010289 - e1010289

Опубликована: Ноя. 21, 2022

The Serotonin Transporter (SERT) regulates extracellular serotonin levels and is the target of most current drugs used to treat depression. mechanisms by which inhibition SERT activity influences behavior are poorly understood. To address this question in model organism Drosophila melanogaster , we developed new loss function mutations ( dSERT) . Previous studies both flies mammals have implicated as an important neuromodulator sleep, our newly generated dSERT mutants show increase total sleep altered architecture that mimicked feeding SSRI citalopram. Differences daytime versus nighttime well genetic rescue experiments unexpectedly suggest distinct serotonergic circuits may modulate sleep. also defects copulation food intake, akin clinical side effects SSRIs consistent with pleomorphic influence on D Starvation did not overcome drive male mutants, mate failed drive. be further explore its interplay other complex behaviors.

Язык: Английский

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Biogenic amines and their receptors in insects

Elsevier eBooks, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Serotonergic modulation across sensory modalities

Journal of Neurophysiology, Год журнала: 2020, Номер 123(6), С. 2406 - 2425

Опубликована: Май 13, 2020

The serotonergic system has been widely studied across animal taxa and different functional networks. This modulatory is therefore well positioned to compare the consequences of neuromodulation for sensory processing species modalities at multiple levels organization. Serotonergic neurons that innervate networks often bidirectionally exchange information with these but also receive input representative motor events or motivational state. convergence supports serotonin’s capacity contextualizing according animal’s physiological state external events. At level circuitry, serotonin can have variable effects due differential projections specific subregions, as receptor type expression within those subregions. Functionally, this infrastructure may gate filter inputs emphasize stimulus features select among streams information. near-ubiquitous presence other neuromodulators regions, coupled their strong on representation, suggests signaling pathways should be considered integral components systems.

Язык: Английский

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Serotonin modulation in the male Aedes aegypti ear influences hearing

Frontiers in Physiology, Год журнала: 2022, Номер 13

Опубликована: Авг. 29, 2022

Male Aedes aegypti (Ae. aegypti) mosquitoes rely on hearing to identify conspecific females for mating, with the male attraction sound of flying ("phonotaxis") an important behavior in initial courtship stage. Hearing thus represents a promising target novel methods mosquito control, and behaviors (such as phonotaxis) can be targeted via use traps. These traps unfortunately have proven relatively ineffective during field deployment. Shifting from function could therefore offer method interfering Ae. mating. Numerous neurotransmitters, including serotonin (5-hydroxytryptamine, or 5-HT) octopamine, are expressed ear, modulation latter influence mechanical responses ear sound. The effect however remains underexplored despite its significant role determining many key biological processes animals. Here we investigated behaviors. Using immunohistochemistry, found expression female ears. In presynaptic sites identified antibody labelling showed only partial overlap serotonin. Next, used RT-qPCR quantify levels three different receptor families (5-HT1, 5-HT2, 5-HT7) heads Although all receptors were ears both sexes, those 5-HT7 family significantly more relative heads. We then thoracically injected serotonin-related compounds into significant, reversible exposure tuning frequency. Finally, oral administration serotonin-synthesis inhibitor altered phonotaxis. serotonergic system represent interesting targets mosquito, disease, control.

Язык: Английский

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Parkinsonian phenotypes induced by Synphilin-1 expression are differentially contributed by serotonergic and dopaminergic circuits and suppressed by nicotine treatment

PLoS ONE, Год журнала: 2023, Номер 18(3), С. e0282348 - e0282348

Опубликована: Март 1, 2023

Synphilin-1 is a protein encoded by the human SNCAIP gene whose function has yet to be fully understood. However, it been linked familial Parkinson's disease (PD). major component of Lewy bodies found in neurons substantia nigra pars compacta PD patients. expression serotonergic and/or dopaminergic Drosophila melanogaster induces neurodegeneration, as well motor and non-motor like symptoms. In this work, we examined contribution circuits development PD-like phenotypes. We that olfactory visual symptoms are majorly contributed system, reduction survival mainly system. Chronic nicotine treatment was able suppress several these These results indicate both systems contribute different aspects symptomatology beneficial effects on specific

Язык: Английский

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Multisensory learning binds modality-specific neurons into a cross-modal memory engram

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Июль 9, 2022

Abstract Associating multiple sensory cues with objects and experience is a fundamental brain process that improves object recognition memory performance. However, neural mechanisms bind features during learning augment expression are unknown. Here we demonstrate multisensory appetitive aversive in Drosophila . Combining colors odors improved performance, even when each modality was tested alone. Temporal control of neuronal function revealed visually-selective mushroom body Kenyon Cells (KCs) to be required for both enhancement visual olfactory after training. Voltage imaging head-fixed flies showed binds activity between streams modality-specific KCs, so unimodal input generates multimodal response. Binding occurs regions the KC axons, which receive valence-relevant dopaminergic reinforcement, propagated downstream. Dopamine locally releases GABA-ergic inhibition permit specific microcircuits within KC-spanning serotonergic neurons as an excitatory bridge previously ‘modality-selective’ streams. Cross-modal binding thereby expands engram by recruiting path KCs become odor responsive. This broadening performance permits single feature retrieve experience.

Язык: Английский

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