Multimodal perception links cellular state to decision-making in single cells

Science, Год журнала: 2022, Номер 377(6606), С. 642 - 648

Опубликована: Июль 14, 2022

Individual cells make decisions that are adapted to their internal state and surroundings, but how can reliably do this remains unclear. To study the information processing capacity of human cells, we conducted multiplexed quantification signaling responses markers cellular state. Signaling nodes in a network displayed adaptive processing, which led heterogeneous growth factor enabled capture partially nonredundant about Collectively, as multimodal percept gives individual large accurately place concentration within context state-dependent decisions. Heterogeneity complexity networks may have coevolved enable specific context-aware decision-making multicellular setting.

Язык: Английский

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The wing imaginal disc

Genetics, Год журнала: 2022, Номер 220(4)

Опубликована: Март 4, 2022

Abstract The Drosophila wing imaginal disc is a tissue of undifferentiated cells that are precursors the and most notum adult fly. first forms during embryogenesis from cluster ∼30 located in second thoracic segment, which invaginate to form sac-like structure. They undergo extensive proliferation larval stages mature ∼35,000 cells. During this time, distinct cell fates assigned different regions, develops complex morphology. Finally, pupal undergoes morphogenetic processes then differentiates notum. While bulk comprises epithelial cells, it also includes neurons glia, associated with tracheal muscle precursor relative simplicity accessibility disc, combined wealth genetic tools available Drosophila, have make premier system for identifying genes deciphering systems play crucial roles animal development. Studies discs made key contributions many areas biology, including patterning, signal transduction, growth control, regeneration, planar polarity, morphogenesis, mechanics.

Язык: Английский

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The Emerging Mechanisms of Wnt Secretion and Signaling in Development

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9

Опубликована: Авг. 16, 2021

Wnts are highly-conserved lipid-modified secreted proteins that activate multiple signaling pathways. These pathways regulate crucial processes during various stages of development and maintain tissue homeostasis in adults. One the most fascinating aspects Wnt protein is despite being hydrophobic, they known to travel several cell distances extracellular space. Research on past four decades has identified factors uncovered mechanisms regulating their expression, secretion, mode travel. More recently, analyses importance gradients growth patterning developing tissues have recognized complex interplay help maintaining homeostasis. This review aims present an overview evidence for modes secretion discuss providing precision robustness tissues.

Язык: Английский

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Tissue shear as a cue for aligning planar polarity in the developing Drosophila wing

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 7, 2025

Abstract Planar polarity establishment in epithelia requires interpretation of directional tissue-level information at cellular and molecular levels. Mechanical forces exerted during tissue morphogenesis are emerging as crucial cues, yet the mechanisms by which they regulate planar poorly understood. Using Drosophila pupal wing, we confirm that stress promotes proximal-distal (PD) alignment. Moreover, high anisotropy can reduce rate accumulation lower stability on cell junctions core protein Frizzled (Fz). Notably, under anisotropy, see an increased gradient flow, characterised differential velocities across adjacent rows. This turnover cell-cell contacts parallel to flow direction, possibly via dissociation transmembrane complexes shear forces. We propose gradients play a critical role establishing maintaining PD-oriented alignment developing wing.

Язык: Английский

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Frizzled-Dependent Planar Cell Polarity without Secreted Wnt Ligands

Developmental Cell, Год журнала: 2020, Номер 54(5), С. 583 - 592.e5

Опубликована: Сен. 1, 2020

Planar cell polarity (PCP) organizes the orientation of cellular protrusions and migratory activity within tissue plane. PCP establishment involves subcellular polarization core components. It has been suggested that Wnt gradients could provide a global cue coordinates local with axes. Here, we dissect role ligands in hairs Drosophila wings, an established system for study PCP. We found was normal quintuple mutant wings rely solely on membrane-tethered Wingless signaling, suggesting gradient is not required. then used nanobody-based approach to trap Wntless endoplasmic reticulum, hence prevent all secretion, specifically during period establishment. still established. conclude that, even though contribute PCP, they are essential, another must exist tissue-wide polarization.

Язык: Английский

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Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7–mediated Vangl ubiquitination and endoplasmic reticulum–associated degradation

Science Advances, Год журнала: 2021, Номер 7(20)

Опубликована: Май 14, 2021

The abundance of core Wnt/PCP protein Vangl is regulated by the endoplasmic reticulum–associated degradation (ERAD) pathway.

Язык: Английский

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How do the Fat–Dachsous and core planar polarity pathways act together and independently to coordinate polarized cell behaviours?

Open Biology, Год журнала: 2021, Номер 11(2)

Опубликована: Фев. 1, 2021

Planar polarity describes the coordinated polarization of cells within plane a tissue. This is controlled by two main pathways in Drosophila : Frizzled-dependent core planar pathway and Fat–Dachsous pathway. Components both these become asymmetrically localized response to long-range upstream cues, form intercellular complexes that link between neighbouring cells. review examines if when are coupled, focusing on wing, eye abdomen. There strong evidence molecularly coupled tissues express specific isoform protein Prickle, namely Spiny-legs. However, other contexts, linkages indirect. We discuss how act together independently mediate diverse range effects cell structures behaviours.

Язык: Английский

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Vangl as a Master Scaffold for Wnt/Planar Cell Polarity Signaling in Development and Disease

Frontiers in Cell and Developmental Biology, Год журнала: 2022, Номер 10

Опубликована: Май 11, 2022

The establishment of polarity within tissues and dynamic cellular morphogenetic events are features common to both developing adult tissues, breakdown these programs is associated with diverse human diseases. Wnt/Planar cell (Wnt/PCP) signaling, a branch non-canonical Wnt critical the maintenance in epithelial as well motility proper embryonic development. In Wnt/PCP-mediated planar relies upon asymmetric distribution core proteins establish polarity, but requirement for this remains unclear. However, polarized migratory cells, Wnt/PCP-specific transmembrane protein Vangl required appears serve scaffold which pathway components positive negative regulators Wnt/PCP signaling assemble. current literature suggests that multiple interaction domains allow binding partners context- tissue-specific complexes. review we discuss role master tissue address how dysregulated disease.

Язык: Английский

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Spatial and temporal regulation of Wnt signaling pathway members in the development of butterfly wing patterns

Science Advances, Год журнала: 2023, Номер 9(30)

Опубликована: Июль 26, 2023

Wnt signaling members are involved in the differentiation of cells associated with eyespot and band color patterns on wings butterflies, but identity spatio-temporal regulation specific pathway remains unclear. Here, we explore localization function Armadillo/β-catenin dependent (canonical) independent (noncanonical) development

Язык: Английский

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Frizzled2 receives WntA signaling during butterfly wing pattern formation

Development, Год журнала: 2023, Номер 150(18)

Опубликована: Авг. 21, 2023

ABSTRACT Butterfly color patterns provide visible and biodiverse phenotypic readouts of the patterning processes. Although secreted ligand WntA has been shown to instruct pattern formation in butterflies, its mode reception remains elusive. genomes encode four homologs Frizzled-family Wnt receptors. Here, we show that CRISPR mosaic knockouts frizzled2 (fz2) phenocopy effects loss function multiple nymphalids. Whereas clones result intermediate reduced size, fz2 are cell-autonomous, consistent with a morphogen function. Shifts expression crispant pupae they under positive negative feedback, respectively. Fz1 is required for Wnt-independent planar cell polarity wing epithelium. Fz3 Fz4 phenotypes competitive-antagonist functions vein (Fz3 Fz4), margin specification (Fz3), Discalis Marginal Band Systems (Fz4). Overall, these data WntA/Frizzled2 morphogen-receptor pair forms signaling axis instructs butterfly shed light on functional diversity insect Frizzled

Язык: Английский

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