Cancer Discovery,
Год журнала:
2022,
Номер
13(2), С. 474 - 495
Опубликована: Окт. 26, 2022
Abstract
The
bone
microenvironment
is
dynamic
and
undergoes
remodeling
in
normal
pathologic
conditions.
Whether
such
affects
disseminated
tumor
cells
(DTC)
metastasis
remains
poorly
understood.
Here,
we
demonstrated
that
fractures
increase
metastatic
colonization
around
the
injury.
NG2+
are
a
common
participant
initiation
both
homeostatic
fractured
mesenchymal
stem/stromal
(BMSC)
often
colocalize
with
DTCs
perivascular
niche.
Both
BMSCs
recruited
to
sites.
Ablation
of
lineage
impaired
concurrently
diminished
colonization.
In
cocultures,
BMSCs,
especially
when
undergoing
osteodifferentiation,
enhanced
cancer
cell
proliferation
migration.
Knockout
N-cadherin
abolished
these
effects
vitro
phenocopied
depletion
vivo.
These
findings
uncover
dual
roles
indicate
osteodifferentiation
promotes
via
N-cadherin–mediated
cell–cell
interaction.
Significance:
occurs
an
environment
constant
remodeling.
Our
study
provides
mechanistic
insights
into
how
homeostasis
repair
lead
outgrowth
cells,
thereby
opening
new
directions
for
further
etiologic
epidemiologic
studies
recurrences.
This
article
highlighted
Issue
feature,
p.
247
Molecular Metabolism,
Год журнала:
2022,
Номер
58, С. 101450 - 101450
Опубликована: Фев. 2, 2022
As
common
progenitor
cells
of
osteoblasts
and
adipocytes,
bone
marrow
mesenchymal
(stromal)
stem
(BMSCs)
play
key
roles
in
homeostasis,
tissue
regeneration,
global
energy
homeostasis;
however,
the
intrinsic
mechanism
BMSC
differentiation
is
not
well
understood.
Plasticity
metabolism
allows
BMSCs
to
match
divergent
demands
osteo-adipogenic
differentiation.
Targeting
metabolic
pathways
may
provide
a
novel
therapeutic
perspective
for
unbalance
related
diseases.
This
review
covers
recent
studies
glucose,
fatty
acids,
amino
acids
fuel
We
also
discuss
findings
about
Glucose,
Moreover,
some
well-known
regulators
including
environmental
stress,
hormone
drugs,
biological
pathological
factors
influence
by
altering
metabolism.
offers
insight
significance
on
fate
determination
provides
possibility
treating
diseases
differentiation,
such
as
obesity
osteoporosis,
from
perspective.
Abstract
The
tissue-resident
skeletal
stem
cells
(SSCs),
which
are
self-renewal
and
multipotent,
continuously
provide
(including
chondrocytes,
bone
cells,
marrow
adipocytes,
stromal
cells)
for
the
development
homeostasis
of
system.
In
recent
decade,
utilizing
fluorescence-activated
cell
sorting,
lineage
tracing,
single-cell
sequencing,
studies
have
identified
various
types
SSCs,
plotted
commitment
trajectory,
partially
revealed
their
properties
under
physiological
pathological
conditions.
this
review,
we
retrospect
to
SSCs
identification
functional
studies.
We
discuss
principles
approaches
identify
bona
fide
highlighting
pioneering
findings
that
plot
atlas
SSCs.
roles
progenitors
in
long
bone,
craniofacial
tissues,
periosteum
systematically
discussed.
further
focus
on
disputes
challenges
SSC
research.
Nature Communications,
Год журнала:
2022,
Номер
13(1)
Опубликована: Янв. 28, 2022
Abstract
Developmental
osteogenesis,
physiological
bone
remodelling
and
fracture
healing
require
removal
of
matrix
cellular
debris.
Osteoclasts
generated
by
the
fusion
circulating
monocytes
degrade
bone,
whereas
identity
cells
responsible
for
cartilage
resorption
is
a
long-standing
controversial
question.
Here
we
show
that
degradation
chondrocyte
phagocytosis
are
mediated
fatty
acid
binding
protein
5-expressing
representing
septoclasts,
which
have
mesenchymal
origin
not
derived
from
haematopoietic
cells.
The
Notch
ligand
Delta-like
4,
provided
endothelial
cells,
necessary
septoclast
specification
developmental
growth.
Consistent
with
termination
growth,
septoclasts
disappear
in
adult
ageing
but
re-emerge
association
growing
vessels
during
healing.
We
propose
rare,
specialized
distinct
osteoclasts.
Our
findings
implications
healing,
frequently
impaired
aging
humans.
Although
previous
RNA
sequencing
methods
have
been
widely
used
in
orthopedic
research
and
provided
ideas
for
therapeutic
strategies,
the
specific
mechanisms
of
some
disorders,
including
osteoarthritis,
lumbar
disc
herniation,
rheumatoid
arthritis,
fractures,
tendon
injuries,
spinal
cord
injury,
heterotopic
ossification,
osteosarcoma,
require
further
elucidation.
The
emergence
single-cell
(scRNA-seq)
technique
has
introduced
a
new
era
on
these
topics,
as
this
method
provides
information
regarding
cellular
heterogeneity,
cell
subtypes,
functions
novel
subclusters,
potential
molecular
mechanisms,
cell-fate
transitions,
cell‒cell
interactions
that
are
involved
development
diseases.
Here,
we
summarize
subpopulations,
genes,
underlying
diseases
identified
by
scRNA-seq,
improving
our
understanding
pathology
providing
insights
into
approaches.
Skeletal
stem
cells
(SSCs)
that
are
capable
of
self-renewal
and
multipotent
differentiation
contribute
to
bone
development
homeostasis.
Several
populations
SSCs
at
different
skeletal
sites
have
been
reported.
Here,
we
identify
a
metaphyseal
SSC
(mpSSC)
population
whose
transcriptional
landscape
is
distinct
from
other
mesenchymal
stromal
(BMSCs).
These
mpSSCs
marked
by
Sstr2
or
Pdgfrb
+
Kitl
−
,
located
just
underneath
the
growth
plate,
exclusively
derived
hypertrophic
chondrocytes
(HCs).
HC-derived
properties
multipotency
in
vitro
vivo,
producing
most
HC
offspring
postnatally.
HC-specific
deletion
Hgs,
component
endosomal
sorting
complex
required
for
transport,
impairs
HC-to-mpSSC
conversion
compromises
trabecular
formation.
Thus,
mpSSC
major
source
BMSCs
osteoblasts
marrow,
supporting
postnatal
