Recent Advances in Single-Cell View of Mesenchymal Stem Cell in Osteogenesis DOI Creative Commons

Fangyuan Shen,

Yu Shi

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 9

Published: Jan. 5, 2022

Osteoblasts continuously replenished by osteoblast progenitor cells form the basis of bone development, maintenance, and regeneration. Mesenchymal stem (MSCs) from various tissues can differentiate into cell osteogenic lineage serve as main source osteoblasts. They also respond flexibly to regenerative anabolic signals emitted surrounding microenvironment, thereby maintaining homeostasis participating in remodeling. However, MSCs exhibit heterogeneity at multiple levels including different tissue sources subpopulations which diversified gene expression differentiation capacity, surface markers used predict potential remain be further elucidated. The rapid advancement tracing methods single-cell technology has made substantial progress characterization stem/progenitor populations MSCs. Here, we reviewed research scRNA-seq identification pathways, MSC-related new insights drawn combined with experimental technology, recent findings regarding interaction between fate niche pathological process.

Language: Английский

The endothelium–bone axis in development, homeostasis and bone and joint disease DOI
Jan Tuckermann, Ralf H. Adams

Nature Reviews Rheumatology, Journal Year: 2021, Volume and Issue: 17(10), P. 608 - 620

Published: Sept. 3, 2021

Language: Английский

Citations

148
Single‐cell transcriptomics of LepR‐positive skeletal cells reveals heterogeneous stress‐dependent stem and progenitor pools DOI Open Access

Chunyang Mo,

Jingxin Guo, Jiachen Qin

et al.

The EMBO Journal, Journal Year: 2021, Volume and Issue: 41(4)

Published: Dec. 27, 2021

Language: Английский

Citations

62
Update on the effects of energy metabolism in bone marrow mesenchymal stem cells differentiation DOI Creative Commons

Kaiting Ning,

Shiqiang Liu,

Baoqiang Yang

et al.

Molecular Metabolism, Journal Year: 2022, Volume and Issue: 58, P. 101450 - 101450

Published: Feb. 2, 2022

As common progenitor cells of osteoblasts and adipocytes, bone marrow mesenchymal (stromal) stem (BMSCs) play key roles in homeostasis, tissue regeneration, global energy homeostasis; however, the intrinsic mechanism BMSC differentiation is not well understood. Plasticity metabolism allows BMSCs to match divergent demands osteo-adipogenic differentiation. Targeting metabolic pathways may provide a novel therapeutic perspective for unbalance related diseases. This review covers recent studies glucose, fatty acids, amino acids fuel We also discuss findings about Glucose, Moreover, some well-known regulators including environmental stress, hormone drugs, biological pathological factors influence by altering metabolism. offers insight significance on fate determination provides possibility treating diseases differentiation, such as obesity osteoporosis, from perspective.

Language: Английский

Citations

59
Insights into skeletal stem cells DOI Creative Commons
Qiwen Li, Ruoshi Xu, Kexin Lei

et al.

Bone Research, Journal Year: 2022, Volume and Issue: 10(1)

Published: Oct. 19, 2022

Abstract The tissue-resident skeletal stem cells (SSCs), which are self-renewal and multipotent, continuously provide (including chondrocytes, bone cells, marrow adipocytes, stromal cells) for the development homeostasis of system. In recent decade, utilizing fluorescence-activated cell sorting, lineage tracing, single-cell sequencing, studies have identified various types SSCs, plotted commitment trajectory, partially revealed their properties under physiological pathological conditions. this review, we retrospect to SSCs identification functional studies. We discuss principles approaches identify bona fide highlighting pioneering findings that plot atlas SSCs. roles progenitors in long bone, craniofacial tissues, periosteum systematically discussed. further focus on disputes challenges SSC research.

Language: Английский

Citations

46
A multi-stem cell basis for craniosynostosis and calvarial mineralization DOI
Seoyeon Bok, Alisha R. Yallowitz, Jun Sun

et al.

Nature, Journal Year: 2023, Volume and Issue: 621(7980), P. 804 - 812

Published: Sept. 20, 2023

Language: Английский

Citations

40
Mesenchymal stromal cell-derived septoclasts resorb cartilage during developmental ossification and fracture healing DOI Creative Commons
Kishor K. Sivaraj, Paul-Georg Majev, Hyun‐Woo Jeong

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Jan. 28, 2022

Abstract Developmental osteogenesis, physiological bone remodelling and fracture healing require removal of matrix cellular debris. Osteoclasts generated by the fusion circulating monocytes degrade bone, whereas identity cells responsible for cartilage resorption is a long-standing controversial question. Here we show that degradation chondrocyte phagocytosis are mediated fatty acid binding protein 5-expressing representing septoclasts, which have mesenchymal origin not derived from haematopoietic cells. The Notch ligand Delta-like 4, provided endothelial cells, necessary septoclast specification developmental growth. Consistent with termination growth, septoclasts disappear in adult ageing but re-emerge association growing vessels during healing. We propose rare, specialized distinct osteoclasts. Our findings implications healing, frequently impaired aging humans.

Language: Английский

Citations

39
Single-cell RNA sequencing in orthopedic research DOI Creative Commons
Tao Wang, Ling Wang, Ping Zhang

et al.

Bone Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: Feb. 24, 2023

Although previous RNA sequencing methods have been widely used in orthopedic research and provided ideas for therapeutic strategies, the specific mechanisms of some disorders, including osteoarthritis, lumbar disc herniation, rheumatoid arthritis, fractures, tendon injuries, spinal cord injury, heterotopic ossification, osteosarcoma, require further elucidation. The emergence single-cell (scRNA-seq) technique has introduced a new era on these topics, as this method provides information regarding cellular heterogeneity, cell subtypes, functions novel subclusters, potential molecular mechanisms, cell-fate transitions, cell‒cell interactions that are involved development diseases. Here, we summarize subpopulations, genes, underlying diseases identified by scRNA-seq, improving our understanding pathology providing insights into approaches.

Language: Английский

Citations

25
YAP and TAZ couple osteoblast precursor mobilization to angiogenesis and mechanoregulation in murine bone development DOI Creative Commons
Joseph M. Collins, Annemarie Lang, Cristian Parisi

et al.

Developmental Cell, Journal Year: 2023, Volume and Issue: 59(2), P. 211 - 227.e5

Published: Dec. 22, 2023

Language: Английский

Citations

24
Identification of the metaphyseal skeletal stem cell building trabecular bone DOI Creative Commons
Guan Yang, Qi He, Xiaoxiao Guo

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(8)

Published: Feb. 23, 2024

Skeletal stem cells (SSCs) that are capable of self-renewal and multipotent differentiation contribute to bone development homeostasis. Several populations SSCs at different skeletal sites have been reported. Here, we identify a metaphyseal SSC (mpSSC) population whose transcriptional landscape is distinct from other mesenchymal stromal (BMSCs). These mpSSCs marked by Sstr2 or Pdgfrb + Kitl − , located just underneath the growth plate, exclusively derived hypertrophic chondrocytes (HCs). HC-derived properties multipotency in vitro vivo, producing most HC offspring postnatally. HC-specific deletion Hgs, component endosomal sorting complex required for transport, impairs HC-to-mpSSC conversion compromises trabecular formation. Thus, mpSSC major source BMSCs osteoblasts marrow, supporting postnatal

Language: Английский

Citations

10
Refining the identity of mesenchymal cell types associated with murine periosteal and endosteal bone DOI Creative Commons
Intawat Nookaew, Jinhu Xiong, Melda Onal

et al.

Journal of Biological Chemistry, Journal Year: 2024, Volume and Issue: 300(4), P. 107158 - 107158

Published: March 11, 2024

Single-cell RNA sequencing has led to novel designations for mesenchymal cells associated with bone as well multiple what appear be the same cell type. The main goals of this study were increase amount single sequence data osteoblasts and osteocytes, compare from periosteum those inside bone, clarify major categories types murine bone. We created an atlas bone-associated by harmonizing published datasets in-house targeted Osx1-Cre Dmp1-Cre driver strains. Cells periosteal analyzed separately isolated endosteum trabecular Over 100,000 mapped reveal 11 clusters designated fibro-1, fibro-2, chondrocytes, articular tenocytes, adipo-CAR, osteo-CAR, pre-osteoblasts, osteoblasts, osteo-X, latter defined in part Postn expression. Osteo-X, pre-osteoblasts closely at surface. Wnt16 was expressed but not endocortical or cancellous Fibro-2 cells, which express markers stem localized adult mice. Suppressing remodeling eliminated altered gene expression did change abundance location osteo-X osteo-CAR cells. These results provide a framework identifying suggest that osteoblast progenitors reside near surface

Language: Английский

Citations

9