Acetyl-CoA-dependent ac4C acetylation promotes the osteogenic differentiation of LPS-stimulated BMSCs

International Immunopharmacology, Год журнала: 2024, Номер 133, С. 112124 - 112124

Опубликована: Апрель 24, 2024

Язык: Английский

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Skeletal Stem/Progenitor Cells in Periosteum and Skeletal Muscle Share a Common Molecular Response to Bone Injury

Journal of Bone and Mineral Research, Год журнала: 2020, Номер 37(8), С. 1545 - 1561

Опубликована: Дек. 1, 2020

ABSTRACT Bone regeneration involves skeletal stem/progenitor cells (SSPCs) recruited from bone marrow, periosteum, and adjacent muscle. To achieve reconstitution after injury, a coordinated cellular molecular response is required these cell populations. Here, we show that SSPCs periosteum muscle are enriched in osteochondral progenitors, more efficiently contribute to endochondral ossification during fracture repair as compared bone-marrow stromal cells. Single-cell RNA sequencing (RNAseq) analyses of periosteal reveal the heterogeneity at steady state fracture. Upon fracture, both transition fibrogenic prior chondrogenesis. This common activation pattern mediated by morphogenetic protein (BMP) signaling. Functionally, Bmpr1a gene inactivation platelet-derived growth factor receptor alpha (Pdgfra)-derived impairs healing decreases SSPC proliferation, migration, differentiation. These results uncover program driving toward regeneration. © 2022 The Authors. Journal Mineral Research published Wiley Periodicals LLC on behalf American Society for (ASBMR).

Язык: Английский

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Functional Heterogeneity of Bone Marrow Mesenchymal Stem Cell Subpopulations in Physiology and Pathology

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 11928 - 11928

Опубликована: Окт. 7, 2022

Bone marrow mesenchymal stem cells (BMSCs) are multi-potent cell populations and capable of maintaining bone body homeostasis. The stemness potential therapeutic effect BMSCs have been explored extensively in recent years. However, diverse surface antigens complex gene expression indicated that represent heterogeneous populations, the natural characteristics make it difficult to identify specific subpopulations pathological processes which often obscured by bulk analysis total BMSCs. Meanwhile, is less effective partly due their heterogeneity. Therefore, understanding functional heterogeneity BMSC under different physiological conditions could major ramifications for global health. Here, we summarize progress physiology pathology. Targeting tissue-resident single subpopulation offers a potentially innovative strategy improves effectiveness clinical application.

Язык: Английский

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Bone Metastasis Initiation Is Coupled with Bone Remodeling through Osteogenic Differentiation of NG2+ Cells

Cancer Discovery, Год журнала: 2022, Номер 13(2), С. 474 - 495

Опубликована: Окт. 26, 2022

Abstract The bone microenvironment is dynamic and undergoes remodeling in normal pathologic conditions. Whether such affects disseminated tumor cells (DTC) metastasis remains poorly understood. Here, we demonstrated that fractures increase metastatic colonization around the injury. NG2+ are a common participant initiation both homeostatic fractured mesenchymal stem/stromal (BMSC) often colocalize with DTCs perivascular niche. Both BMSCs recruited to sites. Ablation of lineage impaired concurrently diminished colonization. In cocultures, BMSCs, especially when undergoing osteodifferentiation, enhanced cancer cell proliferation migration. Knockout N-cadherin abolished these effects vitro phenocopied depletion vivo. These findings uncover dual roles indicate osteodifferentiation promotes via N-cadherin–mediated cell–cell interaction. Significance: occurs an environment constant remodeling. Our study provides mechanistic insights into how homeostasis repair lead outgrowth cells, thereby opening new directions for further etiologic epidemiologic studies recurrences. This article highlighted Issue feature, p. 247

Язык: Английский

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Tracing PRX1+ cells during molar formation and periodontal ligament reconstruction

International Journal of Oral Science, Год журнала: 2022, Номер 14(1)

Опубликована: Янв. 25, 2022

Abstract Neural crest-derived mesenchymal stem cells (MSCs) are known to play an essential function during tooth and skeletal development. PRX1 + constitute important MSC subtype that is implicated in osteogenesis. However, their potential development regeneration remains elusive. In the present study, we first assessed cell fate of molar periodontal ligament (PDL) formation mice. Furthermore, single-cell RNA sequencing analysis was performed study distribution PDL cells. The behavior reconstruction investigated using allogeneic transplanted model. Although spatial specific can differentiate into almost all types molars, third molars highly limited. associated with a high number cells; teeth reconstruction, from recipient alveolar bone participate angiogenesis as pericytes. Overall, key dental MSCs involved mouse pericytes after transplantation.

Язык: Английский

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