ON and OFF starburst amacrine cells are controlled by distinct cholinergic pathways

The Journal of General Physiology, Год журнала: 2024, Номер 156(8)

Опубликована: Июнь 5, 2024

Cholinergic signaling in the retina is mediated by acetylcholine (ACh) released from starburst amacrine cells (SACs), which are key neurons for motion detection. SACs comprise ON and OFF subtypes, morphologically show mirror symmetry to each other. Although many physiological studies on have targeted only, synaptic computation of assumed be similar. Recent demonstrated that gene expression patterns receptor types differed between SACs, suggesting differences their functions. Here, we compared cholinergic pathways mouse using patch clamp technique. The application ACh increased GABAergic feedback, observed as postsynaptic currents both SACs; however, mode feedback differed. Nicotinic receptors while muscarinic only adults. Neither tetrodotoxin, blocked action potentials, nor LY354740, neurotransmitter release eliminated ACh-induced SACs. These results suggest regulated different mechanisms adults non-spiking other than

Язык: Английский

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Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 5, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar-SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Язык: Английский

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Presynaptic depolarization differentially regulates dual neurotransmitter release from starburst amacrine cells in the mouse retina

Frontiers in Ophthalmology, Год журнала: 2023, Номер 3

Опубликована: Авг. 29, 2023

The retina is comprised of diverse neural networks, signaling from photoreceptors to ganglion cells encode images. synaptic connections between these retinal neurons are crucial points for information transfer; however, the input-output relations many synapses understudied. Starburst amacrine in known contribute motion detection circuits, providing a unique window understanding computations. We examined dual transmitter release GABA and acetylcholine starburst by optogenetic activation cells, conducted patch clamp recordings postsynaptic record excitatory inhibitory currents (EPSCs IPSCs). As exhibit distinct kinetics response objects moving preferred or null direction, we mimicked their depolarization using stimuli varying slopes rising phase. amplitudes EPSCs IPSCs were reduced as stimulus speed was prolonged. However, sensitivity slope differed. EPSC consistently steepness phase fell. By contrast, less sensitive rise maintained until became shallow. These results indicate that mechanisms which could cells’ direction selectivity.

Язык: Английский

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eLife assessment: Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells

Опубликована: Сен. 12, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Язык: Английский

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Reviewer #3 (Public Review): Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells

Опубликована: Сен. 12, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Язык: Английский

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