Acetylcholine Promotes Directionally Biased Glutamatergic Retinal Waves

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 15, 2023

Abstract Spontaneous retinal waves are a critical driving force for the self-organization of mouse visual system prior to eye-opening. Classically characterized as taking place in three distinct stages defined by their primary excitatory drive, Stage II during first postnatal week propagated through volume transmission acetylcholine while III second depend on glutamatergic from bipolar cells. However, both late and early share defining propagation bias toward temporal-to-nasal direction despite developmental changes underlying cholinergic networks. Here, we leverage genetic pharmacological manipulations investigate relationship between neurotransmission transition vivo. We find that network continues play vital role after mode glutamate. In absence waves, compensatory activity persists but lacks typically observed waves. gap junction-mediated associated with I throughout window which usually emerge spatiotemporal profile normal including bias. Finally, show signaling β2 subunit-containing nicotinic receptors, essential wave propagation, is also directionality.

Language: Английский

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Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 5, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar-SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Language: Английский

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Presynaptic depolarization differentially regulates dual neurotransmitter release from starburst amacrine cells in the mouse retina

Frontiers in Ophthalmology, Journal Year: 2023, Volume and Issue: 3

Published: Aug. 29, 2023

The retina is comprised of diverse neural networks, signaling from photoreceptors to ganglion cells encode images. synaptic connections between these retinal neurons are crucial points for information transfer; however, the input-output relations many synapses understudied. Starburst amacrine in known contribute motion detection circuits, providing a unique window understanding computations. We examined dual transmitter release GABA and acetylcholine starburst by optogenetic activation cells, conducted patch clamp recordings postsynaptic record excitatory inhibitory currents (EPSCs IPSCs). As exhibit distinct kinetics response objects moving preferred or null direction, we mimicked their depolarization using stimuli varying slopes rising phase. amplitudes EPSCs IPSCs were reduced as stimulus speed was prolonged. However, sensitivity slope differed. EPSC consistently steepness phase fell. By contrast, less sensitive rise maintained until became shallow. These results indicate that mechanisms which could cells’ direction selectivity.

Language: Английский

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eLife assessment: Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells

Published: Sept. 12, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Language: Английский

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Reviewer #3 (Public Review): Heterogeneous presynaptic receptive fields contribute to directional tuning in starburst amacrine cells

Published: Sept. 12, 2023

The processing of visual information by retinal starburst amacrine cells (SACs) involves transforming excitatory input from bipolar (BCs) into directional calcium output. While previous studies have suggested that an asymmetry in the kinetic properties along soma-dendritic axes postsynaptic cell could enhance tuning at level individual branches, it remains unclear whether biologically relevant presynaptic kinetics contribute to direction selectivity when stimulation engages entire dendritic tree. To address this question, we built multicompartmental models bipolar–SAC circuit and trained them boost tuning. We report despite significant crosstalk dissimilar preferences dendrites occur during whole-cell stimulation, rules guide BC leading optimal are similar single-dendrite condition. correlate model predictions empirical findings, utilized two-photon glutamate imaging study dynamics release onto ON- OFF-starburst murine retina. reveal diverse response motion both populations; algorithms on experimental data differences temporal likely correspond heterogeneous receptive field (RF) among different types, including spatial extent center surround components. In addition, demonstrate architecture composed units with experimentally recorded drive but not levels replicate suggesting other DS mechanisms required explain SAC function. Our provides new insights complex underlying highlights potential contribution computation cells.

Language: Английский

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