Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 21, 2023
Abstract
The
recovery
of
mitochondrial
quality
control
(MQC)
may
bring
innovative
solutions
for
neuroprotection,
while
also
imposing
an
extraordinary
challenge
given
the
need
holistic
approaches
to
restore
dynamics
(fusion/fission)
and
turnover
(mitophagy
biogenesis).
In
Diabetic
Retinopathy
(DR)
this
therapeutic
approach
is
further
compounded
by
our
lack
understanding
human
retinal
neurodegeneration,
but
how
MQC
processes
interplay
during
disease
progression.
Here,
we
show
first
time
that
mitochondria
remodel
towards
hyperfusion
neurodegeneration
in
murine
diabetes.
Using
Mito-QC
reporter,
demonstrate
blunts
homeostatic
diabetes,
causing
metabolic,
neuronal,
neuro-inflammatory
(glial)
stress.
By
mimicking
remodelling
vitro
,
generated
a
drug
discovery
platform
whereby
glycosylated
N6-furfuryladenine(s)
(but
not
other
PINK1-activators)
restored
cellular
bioenergetics
relaxing
controlled
fashion.
Oral
administration
N6-furfuryladenine
(Ins2
Akita
)
diabetic
retina,
improving
clinical
correlates
(electroretinogram
SD-OCT)
conferring
human-relevant
neuroprotection
regardless
glycaemic
status.
Collectively,
study
provides
new
translational
insights
retina
through
MQC.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 11, 2024
Abstract
Here
we
show
that
under
oxygen-glucose
deprivation
(OGD),
multiple
myeloma
cells
transfer
dysfunctional
mitochondria
to
bone
marrow
stromal
(BMSCs)
for
degradation
(transmitophagy).
These
are
then
transferred
between
BMSCs
via
tunneling
nanotubes
contributing
survival
in
OGD.
Tissue Engineering Part A,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 10, 2024
The
objective
of
this
study
is
to
investigate
the
influence
exogenous
mitochondria
(Mt)
internalization
on
Mt
membrane
potential
cells.
Cationized
gelatin
nanospheres
(cGNS)
were
prepared
mix
at
different
ratios
prepare
associated
with
cGNS
(Mt-cGNS).
depended
Mt/cGNS
mixing
ratio
achieve
maximum
3/1.
Rho
0
cells
a
function-deficient
line
evaluate
enhancement
rho
after
Mt-cGNS.
When
evaluated
by
using
tetramethylrhodamine
methyl
ester
reagent,
mitochondrial
incubation
Mt-cGNS
enhanced
compared
that
incubated
only
and
maintained
significantly
higher
level
even
for
6
days.
internalized
into
an
actin-dependent
pathway,
followed
fused
endogenous
Mt.
It
concluded
association
enabled
enhance
cellular
internalization,
fusion
maintain
potential.
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 21, 2023
Abstract
The
recovery
of
mitochondrial
quality
control
(MQC)
may
bring
innovative
solutions
for
neuroprotection,
while
also
imposing
an
extraordinary
challenge
given
the
need
holistic
approaches
to
restore
dynamics
(fusion/fission)
and
turnover
(mitophagy
biogenesis).
In
Diabetic
Retinopathy
(DR)
this
therapeutic
approach
is
further
compounded
by
our
lack
understanding
human
retinal
neurodegeneration,
but
how
MQC
processes
interplay
during
disease
progression.
Here,
we
show
first
time
that
mitochondria
remodel
towards
hyperfusion
neurodegeneration
in
murine
diabetes.
Using
Mito-QC
reporter,
demonstrate
blunts
homeostatic
diabetes,
causing
metabolic,
neuronal,
neuro-inflammatory
(glial)
stress.
By
mimicking
remodelling
vitro
,
generated
a
drug
discovery
platform
whereby
glycosylated
N6-furfuryladenine(s)
(but
not
other
PINK1-activators)
restored
cellular
bioenergetics
relaxing
controlled
fashion.
Oral
administration
N6-furfuryladenine
(Ins2
Akita
)
diabetic
retina,
improving
clinical
correlates
(electroretinogram
SD-OCT)
conferring
human-relevant
neuroprotection
regardless
glycaemic
status.
Collectively,
study
provides
new
translational
insights
retina
through
MQC.
