Relaxation of mitochondrial hyperfusion in the diabetic retina via N6-furfuryladenine confers neuroprotection regardless of glycaemic status. DOI Creative Commons
Aidan Anderson, Nada Alfahad,

Dulani Wimalachandra

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 21, 2023

Abstract The recovery of mitochondrial quality control (MQC) may bring innovative solutions for neuroprotection, while also imposing an extraordinary challenge given the need holistic approaches to restore dynamics (fusion/fission) and turnover (mitophagy biogenesis). In Diabetic Retinopathy (DR) this therapeutic approach is further compounded by our lack understanding human retinal neurodegeneration, but how MQC processes interplay during disease progression. Here, we show first time that mitochondria remodel towards hyperfusion neurodegeneration in murine diabetes. Using Mito-QC reporter, demonstrate blunts homeostatic diabetes, causing metabolic, neuronal, neuro-inflammatory (glial) stress. By mimicking remodelling vitro , generated a drug discovery platform whereby glycosylated N6-furfuryladenine(s) (but not other PINK1-activators) restored cellular bioenergetics relaxing controlled fashion. Oral administration N6-furfuryladenine (Ins2 Akita ) diabetic retina, improving clinical correlates (electroretinogram SD-OCT) conferring human-relevant neuroprotection regardless glycaemic status. Collectively, study provides new translational insights retina through MQC.

Language: Английский

Tunneling Nanotubes Between Bone Marrow Stromal Cells Support Transmitophagy and Resistance to Apoptosis in Myeloma DOI Creative Commons
Antonio Giovanni Solimando, Francesco Di Palma, Vanessa Desantis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Abstract Here we show that under oxygen-glucose deprivation (OGD), multiple myeloma cells transfer dysfunctional mitochondria to bone marrow stromal (BMSCs) for degradation (transmitophagy). These are then transferred between BMSCs via tunneling nanotubes contributing survival in OGD.

Language: Английский

Citations

0
Association with Cationized Gelatin Nanospheres Enhances Mitochondria Uptake and Membrane Potential DOI

Wenxuan Yang,

Satoshi Abe, Mitsuru Ando

et al.

Tissue Engineering Part A, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

The objective of this study is to investigate the influence exogenous mitochondria (Mt) internalization on Mt membrane potential cells. Cationized gelatin nanospheres (cGNS) were prepared mix at different ratios prepare associated with cGNS (Mt-cGNS). depended Mt/cGNS mixing ratio achieve maximum 3/1. Rho 0 cells a function-deficient line evaluate enhancement rho after Mt-cGNS. When evaluated by using tetramethylrhodamine methyl ester reagent, mitochondrial incubation Mt-cGNS enhanced compared that incubated only and maintained significantly higher level even for 6 days. internalized into an actin-dependent pathway, followed fused endogenous Mt. It concluded association enabled enhance cellular internalization, fusion maintain potential.

Language: Английский

Citations

0
How crosstalk between mitochondria, lysosomes, and other organelles can prevent or promote dry age-related macular degeneration DOI
Aparna Lakkaraju, Patricia Boya, Marie Csete

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: unknown, P. 110219 - 110219

Published: Dec. 1, 2024

Language: Английский

Citations

0
Targeting mitochondrial transfer: a new horizon in cardiovascular disease treatment DOI Creative Commons

Baile Zuo,

Xiaoyan Li,

Dawei Xu

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 31, 2024

Language: Английский

Citations

0
Relaxation of mitochondrial hyperfusion in the diabetic retina via N6-furfuryladenine confers neuroprotection regardless of glycaemic status. DOI Creative Commons
Aidan Anderson, Nada Alfahad,

Dulani Wimalachandra

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: July 21, 2023

Abstract The recovery of mitochondrial quality control (MQC) may bring innovative solutions for neuroprotection, while also imposing an extraordinary challenge given the need holistic approaches to restore dynamics (fusion/fission) and turnover (mitophagy biogenesis). In Diabetic Retinopathy (DR) this therapeutic approach is further compounded by our lack understanding human retinal neurodegeneration, but how MQC processes interplay during disease progression. Here, we show first time that mitochondria remodel towards hyperfusion neurodegeneration in murine diabetes. Using Mito-QC reporter, demonstrate blunts homeostatic diabetes, causing metabolic, neuronal, neuro-inflammatory (glial) stress. By mimicking remodelling vitro , generated a drug discovery platform whereby glycosylated N6-furfuryladenine(s) (but not other PINK1-activators) restored cellular bioenergetics relaxing controlled fashion. Oral administration N6-furfuryladenine (Ins2 Akita ) diabetic retina, improving clinical correlates (electroretinogram SD-OCT) conferring human-relevant neuroprotection regardless glycaemic status. Collectively, study provides new translational insights retina through MQC.

Language: Английский

Citations

0