The
claustrum
is
thought
to
be
one
of
the
most
highly
interconnected
forebrain
structures
but
its
organizing
principles
have
yet
fully
explored
at
level
single
neurons.
Here,
we
investigated
identity,
connectivity,
and
activity
identified
neurons
understand
how
structure’s
unique
convergence
input
divergence
output
support
binding
information
streams.
We
found
that
in
communicate
with
each
other
across
efferent
projection-defined
modules
which
were
differentially
innervated
by
sensory
frontal
cortical
areas.
Individual
responsive
inputs
from
more
than
region
a
cell-type
projection-specific
manner,
particularly
between
areas
cortex.
In
vivo
imaging
axons
revealed
responses
both
unimodal
multimodal
stimuli.
Finally,
chronic
silencing
specifically
reduced
animals’
sensitivity
These
findings
view
fundamentally
integrative
structure,
consolidating
around
cortex
redistributing
it
following
local
computations.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 14, 2024
ABSTRACT
The
claustrum
(CLA),
a
subcortical
nucleus
in
mammals,
essentially
composed
of
excitatory
projection
neurons
and
known
for
its
extensive
connections
with
the
neocortex,
has
recently
been
associated
variety
functions
ranging
from
consciousness
to
impulse
control.
However,
research
on
CLA
challenging
due
difficulties
specifically
comprehensively
targeting
neuronal
populations.
In
various
cases,
this
limitation
led
inconsistent
findings
lack
reliable
data.
present
work,
we
describe
expression
profile
Smim32
gene,
which
is
almost
exclusively
transcribed
endopiriform
nucleus,
as
well
inhibitory
thalamic
reticular
nucleus.
Leveraging
unique
pattern,
developed
series
Cre-
Flippase-expressing
knockin
BAC
transgenic
mouse
lines
different
profiles.
With
these
novel
tools
hand,
propose
new
standards
interrogation
function.
The
claustrum
(CLA),
a
subcortical
nucleus
in
mammals,
essentially
composed
of
excitatory
projection
neurons
and
known
for
its
extensive
connections
with
the
neocortex,
has
recently
been
associated
variety
functions
ranging
from
consciousness
to
impulse
control.
However,
research
on
CLA
challenging
due
difficulties
specifically
comprehensively
targeting
neuronal
populations.
In
various
cases,
this
limitation
led
inconsistent
findings
lack
reliable
data.
present
work,
we
describe
expression
profile
Smim32
gene,
which
is
almost
exclusively
transcribed
endopiriform
nucleus,
as
well
inhibitory
thalamic
reticular
nucleus.
Leveraging
unique
pattern,
developed
series
Cre-
Flippase-expressing
knockin
BAC
transgenic
mouse
lines
different
profiles.
With
these
novel
tools
hand,
propose
new
standards
interrogation
function.
The
claustrum
(CLA),
a
subcortical
nucleus
in
mammals,
essentially
composed
of
excitatory
projection
neurons
and
known
for
its
extensive
connections
with
the
neocortex,
has
recently
been
associated
variety
functions
ranging
from
consciousness
to
impulse
control.
However,
research
on
CLA
challenging
due
difficulties
specifically
comprehensively
targeting
neuronal
populations.
In
various
cases,
this
limitation
led
inconsistent
findings
lack
reliable
data.
present
work,
we
describe
expression
profile
Smim32
gene,
which
is
almost
exclusively
transcribed
endopiriform
nucleus,
as
well
inhibitory
thalamic
reticular
nucleus.
Leveraging
unique
pattern,
developed
series
Cre-
Flippase-expressing
knockin
BAC
transgenic
mouse
lines
different
profiles.
With
these
novel
tools
hand,
propose
new
standards
interrogation
function.
Human Brain Mapping,
Год журнала:
2024,
Номер
45(14)
Опубликована: Окт. 1, 2024
ABSTRACT
Despite
substantial
neuroscience
research
in
the
last
decade
revealing
claustrum's
prominent
role
mammalian
forebrain
organization,
as
evidenced
by
its
extraordinarily
widespread
connectivity
pattern,
claustrum
studies
humans
are
rare.
This
is
particularly
true
for
focusing
on
connections.
Two
primary
reasons
may
account
this
situation:
First,
intricate
anatomy
of
human
located
between
external
and
extreme
capsule
hinders
straightforward
reliable
structural
delineation.
In
addition,
few
that
used
diffusion‐weighted‐imaging
(DWI)‐based
tractography
could
not
clarify
whether
vivo
consistently
reliably
identifies
connections
across
different
subjects,
cohorts,
imaging
methods,
metrics.
To
address
these
issues,
we
combined
a
recently
developed
deep‐learning‐based
segmentation
tool
with
DWI‐based
two
large
adult
cohorts:
81
healthy
young
adults
from
connectome
project
further
participants
Bavarian
longitudinal
study.
Tracts
13
cortical
9
subcortical
regions
were
reconstructed
each
subject
using
probabilistic
tractography.
Probabilistic
group
average
maps
metrics
generated
to
assess
profile
well
consistency
replicability
We
found,
individuals,
DWI‐protocols,
measures,
consistent
replicable
ipsi‐
contralateral
result
demonstrates
robust
humans,
providing
base
examinations
health
disease.
The
claustrum
is
thought
to
be
one
of
the
most
highly
interconnected
forebrain
structures
but
its
organizing
principles
have
yet
fully
explored
at
level
single
neurons.
Here,
we
investigated
identity,
connectivity,
and
activity
identified
neurons
understand
how
structure’s
unique
convergence
input
divergence
output
support
binding
information
streams.
We
found
that
in
communicate
with
each
other
across
efferent
projection-defined
modules
which
were
differentially
innervated
by
sensory
frontal
cortical
areas.
Individual
responsive
inputs
from
more
than
region
a
cell-type
projection-specific
manner,
particularly
between
areas
cortex.
In
vivo
imaging
axons
revealed
responses
both
unimodal
multimodal
stimuli.
Finally,
chronic
silencing
specifically
reduced
animals’
sensitivity
These
findings
view
fundamentally
integrative
structure,
consolidating
around
cortex
redistributing
it
following
local
computations.
