Cell-type-specific origins of locomotor rhythmicity at different speeds in larval zebrafish DOI Open Access
Moneeza A. Agha, S. Kishore, David L. McLean

и другие.

Опубликована: Июль 11, 2024

Different speeds of locomotion require heterogeneous spinal populations, but a common mode rhythm generation is presumed to exist. Here, we explore the cellular versus synaptic origins rhythmicity at different by performing electrophysiological recordings from premotor excitatory interneurons in larval zebrafish. Chx10-labeled V2a neurons are divided into least two morphological subtypes proposed play distinct roles timing and intensity control. Consistent with generating output patterning functions within population, find that descending recruited exclusively slow or fast exhibit intrinsic properties suitable for rhythmogenesis those speeds, while bifurcating more reliably all lack appropriate rhythmogenic properties. Unexpectedly, however, phasic firing patterns during non-rhythmogenic alike best explained modes inhibition linked cell-type speed. At reciprocal supports firing, recurrent helps pattern motor output. In contrast, rely on alone Our findings suggest cell-type-specific, not common, generate coordinate locomotion.

Язык: Английский

Molecular blueprints for spinal circuit modules controlling locomotor speed in zebrafish DOI Creative Commons
Irene Pallucchi, Maria Bertuzzi,

David Madrid

и другие.

Nature Neuroscience, Год журнала: 2023, Номер 27(1), С. 78 - 89

Опубликована: Ноя. 2, 2023

Abstract The flexibility of motor actions is ingrained in the diversity neurons and how they are organized into functional circuit modules, yet our knowledge molecular underpinning modularity remains limited. Here we use adult zebrafish to link motoneurons (MNs) rhythm-generating V2a interneurons (INs) with modular organization that responsible for changes locomotor speed. We show MNs INs reflects their segregation slow, intermediate or fast subtypes. Furthermore, reveal shared signatures between three speed modules. Overall, by characterizing relates function, connectivity behavior, study provides important insights not only mechanisms neuronal but also charting circuits general.

Язык: Английский

Процитировано

20
Temporal patterning of the vertebrate developing neural tube DOI Creative Commons
Andreas Sagner

Current Opinion in Genetics & Development, Год журнала: 2024, Номер 86, С. 102179 - 102179

Опубликована: Март 14, 2024

The chronologically ordered generation of distinct cell types is essential for the establishment neuronal diversity and formation circuits. Recently, single-cell transcriptomic analyses various areas developing vertebrate nervous system have provided evidence existence a shared temporal patterning program that partitions neurons based on timing neurogenesis. In this review, I summarize findings lead to proposal before focusing spinal cord discuss how in general specifically contributes

Язык: Английский

Процитировано

7
Engineering Innervated Musculoskeletal Tissues for Regenerative Orthopedics and Disease Modeling DOI Creative Commons

Zhilong Zhou,

Jun Liu,

Tiandi Xiong

и другие.

Small, Год журнала: 2024, Номер 20(23)

Опубликована: Янв. 10, 2024

Abstract Musculoskeletal (MSK) disorders significantly burden patients and society, resulting in high healthcare costs productivity loss. These are the leading cause of physical disability, their prevalence is expected to increase as sedentary lifestyles become common global population elderly increases. Proper innervation critical maintaining MSK function, nerve damage or dysfunction underlies various disorders, underscoring potential restoring function disorder treatment. However, most tissue engineering strategies have overlooked significance innervation. This review first expounds upon system its importance homeostasis functions. will be followed by for tissues that induce post‐implantation situ pre‐innervated. Subsequently, research progress modeling using innervated organoids organs‐on‐chips (OoCs) analyzed. Finally, future development treat recapitulate disease mechanisms discussed. provides valuable insights into underlying principles, methods, applications tissues, paving way targeted, efficacious therapies conditions.

Язык: Английский

Процитировано

6
The vestibulospinal nucleus is a locus of balance development DOI
Kyla R. Hamling, Katherine Harmon, Yukiko Kimura

и другие.

Journal of Neuroscience, Год журнала: 2024, Номер 44(30), С. e2315232024 - e2315232024

Опубликована: Май 22, 2024

Mature vertebrates maintain posture using vestibulospinal neurons that transform sensed instability into reflexive commands to spinal motor circuits. Postural stability improves across development. However, due the complexity of terrestrial locomotion, contributions postural refinement in early life remain unexplored. Here we leveraged relative simplicity underwater locomotion quantify consequences losing during development larval zebrafish undifferentiated sex. By comparing at two timepoints, discovered later lesions led greater instability. Analysis thousands individual swim bouts revealed disrupted movement timing and corrective reflexes without impacting kinematics, this effect was particularly strong older larvae. Using a generative model swimming, showed how these disruptions could account for increased variability both timepoints. Finally, late fin/trunk coordination observed larvae, linking control schemes used navigate depth. Since were considerably more disruptive stability, conclude balance increase as larvae mature. Vestibulospinal are highly conserved vertebrates; therefore propose they substrate developmental improvements control.

Язык: Английский

Процитировано

4
Sensation is dispensable for the maturation of the vestibulo-ocular reflex DOI
Paige Leary, Celine Bellegarda,

Cheryl Quainoo

и другие.

Science, Год журнала: 2025, Номер 387(6729), С. 85 - 90

Опубликована: Янв. 2, 2025

Vertebrates stabilize gaze using a neural circuit that transforms sensed instability into compensatory counterrotation of the eyes. Sensory feedback tunes this vestibulo-ocular reflex throughout life. We studied functional development components in larval zebrafish, with and without sensation. Blind fish normally, responses to body tilts mature before behavior. In contrast, synapses between motor neurons eye muscles time course similar behavioral maturation. Larvae vestibular sensory experience, but neuromuscular junctions, had strong reflex. Development junction, not therefore determines rate maturation an ancient

Язык: Английский

Процитировано

0
Cerebellar Purkinje Cells Control Posture in Larval Zebrafish (Danio rerio) DOI Open Access
Franziska Auer, Katherine Nardone, Kōji Matsuda

и другие.

Опубликована: Янв. 24, 2025

Cerebellar dysfunction leads to postural instability. Recent work in freely moving rodents has transformed investigations of cerebellar contributions posture. However, the combined complexity terrestrial locomotion and rodent cerebellum motivate new approaches perturb function simpler vertebrates. Here, we adapted a validated chemogenetic tool (TRPV1/capsaicin) describe role Purkinje cells — output neurons cortex as larval zebrafish swam depth. We achieved both bidirectional control (activation ablation) while performing quantitative high-throughput assessment posture locomotion. Activation modified pitch (nose-up/nose-down) axis. Similarly, ablations disrupted pitch-axis fin-body coordination responsible for climbs. Postural disruption was more widespread older larvae, offering window into emergent roles developing Finally, found that activity could individually collectively encode tilt direction, key feature neurons. Our findings delineate an expected vestibular sensation zebrafish, establishing validity TRPV1/capsaicin-mediated perturbations simple, genetically-tractable vertebrate. Moreover, by comparing cell time, uncover signatures emerging across early development. This takes major step towards understanding ancestral regulating maturation.

Язык: Английский

Процитировано

0
Saccade subtypes: Eyes on the prize DOI
Celine Bellegarda, David Schoppik

Current Biology, Год журнала: 2025, Номер 35(3), С. R89 - R91

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
An ultrastructural map of a spinal sensorimotor circuit reveals the potential of astroglia modulation DOI Creative Commons

Zachary M. Koh,

Ricky Avalos Arceo,

Jacob Hammer

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

Information flow through circuits is dictated by the precise connectivity of neurons and glia. While a single astrocyte can contact many synapses, how glial-synaptic interactions are arranged within circuit to impact information remains understudied. Here, we use local spinal sensorimotor in zebrafish as model understand astroglia connected vertebrate circuit. With semi-automated cellular reconstructions automated approaches map all synaptic connections, identified connections circuit, from dorsal root ganglia interneurons finally motor neurons. This revealed complex network that interact We then mapped glial processes tripartite synapses demonstrate equally distributed across supporting idea glia modulate at different levels. show multiple astroglia, including bona fide astrocytes, sensory neuron's each these parts detailed reveals an extensive process stimuli vertebrate. utilized this ultrastructural thresholding modulation could alter circuits. validated with GCaMP6s imaging ganglia, astroglia. work provides foundational resource detailing organization offering insights influence neural networks.

Язык: Английский

Процитировано

0
Cerebellar Purkinje cells control posture in larval zebrafish (Danio rerio) DOI Creative Commons
Franziska Auer, Katherine Nardone, Kōji Matsuda

и другие.

eLife, Год журнала: 2025, Номер 13

Опубликована: Апрель 3, 2025

Cerebellar dysfunction leads to postural instability. Recent work in freely moving rodents has transformed investigations of cerebellar contributions posture. However, the combined complexity terrestrial locomotion and rodent cerebellum motivate new approaches perturb function simpler vertebrates. Here, we adapted a validated chemogenetic tool (TRPV1/capsaicin) describe role Purkinje cells — output neurons cortex as larval zebrafish swam depth. We achieved both bidirectional control (activation ablation) while performing quantitative high-throughput assessment posture locomotion. Activation modified pitch (nose-up/nose-down) axis. Similarly, ablations disrupted pitch-axis fin-body coordination responsible for climbs. Postural disruption was more widespread older larvae, offering window into emergent roles developing Finally, found that activity could individually collectively encode tilt direction, key feature neurons. Our findings delineate an expected vestibular sensation zebrafish, establishing validity TRPV1/capsaicin-mediated perturbations simple, genetically tractable vertebrate. Moreover, by comparing cell time, uncover signatures emerging across early development. This takes major step towards understanding ancestral regulating maturation.

Язык: Английский

Процитировано

0
Cell-type-specific origins of locomotor rhythmicity at different speeds in larval zebrafish DOI Creative Commons
Moneeza A. Agha, S. Kishore, David L. McLean

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Фев. 7, 2024

Different speeds of locomotion require heterogeneous spinal populations, but a common mode rhythm generation is presumed to exist. Here, we explore the cellular versus synaptic origins rhythmicity at different by performing electrophysiological recordings from premotor excitatory interneurons in larval zebrafish. Chx10-labeled V2a neurons are divided into least two morphological subtypes proposed play distinct roles timing and intensity control. Consistent with generating output patterning functions within population, find that descending recruited exclusively slow or fast exhibit intrinsic properties suitable for rhythmogenesis those speeds, while bifurcating more reliably all lack appropriate rhythmogenic properties. Unexpectedly, however, phasic firing patterns during non-rhythmogenic alike best explained modes inhibition linked cell type speed. At reciprocal supports firing, recurrent helps pattern motor output. In contrast, rely on alone Our findings suggest cell-type-specific, not common, generate coordinate locomotion.

Язык: Английский

Процитировано

2