bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 14, 2023
Abstract
Environmental
risk
assessment
traditionally
relies
on
a
wide
range
of
in
vivo
testing
to
assess
the
potential
hazard
chemicals
environment.
These
tests
are
often
time-consuming,
costly
and
can
cause
test
organisms’
suffering.
Recent
developments
reliable
low-cost
alternatives,
both
-
silico
-based,
opened
door
reconsider
current
toxicity
assessment.
However,
many
these
new
approach
methodologies
(NAMs)
rely
high
quality
annotated
genomes
for
surrogate
species
regulatory
Currently,
lacking
genomic
information
slows
process
NAM
development.
Here,
we
present
phylogenetically
resolved
overview
missing
resources
within
ecotoxicological
We
call
an
organized
systematic
effort
(regulatory)
community
provide
resources.
Further,
discuss
standardized
landscape
enable
robust
non-animal
reliant
systems
ecotoxicology
era.
Synopsis
Statement
identify
needed
development
acceptance
environmental
Environmental Science & Technology,
Год журнала:
2024,
Номер
58(4), С. 1877 - 1881
Опубликована: Янв. 21, 2024
Environmental
risk
assessment
traditionally
relies
on
a
wide
range
of
in
vivo
testing
to
assess
the
potential
hazards
chemicals
environment.
These
tests
are
often
time-consuming
and
costly
can
cause
test
organisms'
suffering.
Recent
developments
reliable
low-cost
alternatives,
both
vivo-
silico-based,
opened
door
reconsider
current
toxicity
assessment.
However,
many
these
new
approach
methodologies
(NAMs)
rely
high-quality
annotated
genomes
for
surrogate
species
regulatory
Currently,
lack
genomic
information
slows
process
NAM
development.
Here,
we
present
phylogenetically
resolved
overview
missing
resources
within
ecotoxicological
We
call
an
organized
systematic
effort
(regulatory)
community
provide
resources.
Further,
discuss
standardized
landscape
enable
robust
nonanimal-reliant
systems
ecotoxicology
era.
Mutagenesis,
Год журнала:
2024,
Номер
39(2), С. 69 - 77
Опубликована: Фев. 1, 2024
Chemical
safety
testing
plays
a
crucial
role
in
product
and
pharmacological
development,
as
well
chemoprevention;
however,
vitro
genotoxicity
tests
do
not
always
accurately
predict
the
chemicals
that
will
be
vivo
carcinogens.
If
test
positive
for
but
negative
vivo,
this
can
contribute
to
unnecessary
animals
used
confirm
erroneous
results.
Current
typically
evaluate
only
endpoints,
which
limits
their
potential
detect
non-genotoxic
The
frequency
of
misleading
results
high,
leading
requirement
more
informative
tests.
It
is
now
recognized
multiple-endpoint
may
aid
accurate
detection
carcinogens
non-carcinogens.
objective
review
was
utility
our
novel,
test,
uses
multiple
cancer-relevant
endpoints
carcinogenic
potential.
tool
assessed
micronucleus
frequency,
p53
expression,
p21
mitochondrial
respiration,
cell
cycle
abnormalities
and,
uniquely,
morphology
changes
human
lymphoblastoid
lines,
TK6
MCL-5.
were
observe
cellular
responses
18
within
following
categories:
genotoxic
carcinogens,
toxic
non-carcinogens,
agents.
number
significantly
altered
each
chemical
considered,
alongside
holistic
Integrated
Signature
Carcinogenicity
score,
derived
from
sum
fold
all
endpoints.
Following
calculation
an
overall
score
these
measures,
exhibited
greater
potency
than
Genotoxic
generally
potent
This
novel
approach
therefore
demonstrated
correctly
predicting
whether
with
unknown
mechanism
considered
Overall,
while
further
validation
recommended,
demonstrates
identification
compounds.
Adoption
could
enable
reduced
animal
use
carcinogenicity
testing.
Biosensors and Bioelectronics,
Год журнала:
2024,
Номер
261, С. 116454 - 116454
Опубликована: Май 31, 2024
Several
organ-on-chip
and
cell-on-chip
devices
have
been
reported,
however,
their
main
drawback
is
that
they
are
not
interoperable
(i.e.,
fabricated
with
customized
equipment,
thus
cannot
be
applied
in
other
facilities,
unless
having
the
same
setup),
require
cell-culture
facilities
benchtop
instrumentation.
As
a
consequence,
results
obtained
such
do
generally
comply
principles
of
findability,
accessibility,
interoperability,
reusability
(FAIR).
To
overcome
limitation,
leveraging
cost-effective
3D
printing
we
developed
bioluminescent
tissue
on-a-chip
device
can
easily
implemented
any
laboratory.
The
enables
continuous
monitoring
cell
co-cultures
expressing
different
reporter
proteins
and,
thanks
to
implementation
new
highly
luciferases
high
pH
thermal
stability,
monitored
via
smartphone
camera.
Another
relevant
feature
possibility
insert
chip
into
commercial
24-well
plate
for
use
standard
suitability
this
cell-based
biosensing
activation
target
molecular
pathways,
i.e.,
inflammatory
pathway
nuclear
factor
kappa-B
(NF-κB)
activation,
general
cytotoxicity
here
reported
showing
similar
analytical
performance
when
compared
conventional
assays
performed
plates.
Analytical Methods,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 10, 2024
A
novel
fluorescence
spectroscopy
method
that
can
be
used
as
a
low
resource,
rapid,
and
green
approach
for
measuring
chemical
losses
in
polystyrene
microplates
commonly
bioassays.
