Toxicology Effects of Cadmium in Pomacea canaliculate: Accumulation, Oxidative Stress, Microbial Community, and Transcriptome Analysis
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(2), С. 751 - 751
Опубликована: Янв. 17, 2025
Cadmium
(Cd)
pollution
poses
an
important
problem,
but
limited
information
is
available
about
the
toxicology
effects
of
Cd
on
freshwater
invertebrates.
We
investigated
accumulation,
oxidative
stress,
microbial
community
changes,
and
transcriptomic
alterations
in
apple
snails
(Pomacea
canaliculata)
under
stress.
The
were
exposed
to
10
μg/L
solution
for
16
days,
followed
by
a
16-day
elimination
period.
Our
results
showed
that
liver
accumulated
highest
concentration
(17.41
μg/g),
kidneys
(8.00
μg/g)
intestine-stomach
(6.68
highlighting
these
tissues
as
primary
targets
accumulation.
During
period,
concentrations
decreased
all
tissues,
with
head-foot
shell
exhibiting
over
30%
rates.
stress
also
resulted
reduced
activities
superoxide
dismutase
(SOD),
catalase
(CAT),
glutathione
transferase
(GST)
compared
control
group.
Notably,
even
after
days
depuration,
enzyme
did
not
return
normal
levels,
indicating
persistent
toxicological
effects.
exposure
significantly
diversity
gut
microbiota
P.
canaliculata.
Moreover,
transcriptome
analysis
identified
differentially
expressed
genes
(DEGs)
primarily
associated
lysosome
function,
motor
proteins,
protein
processing
endoplasmic
reticulum,
drug
metabolism
via
cytochrome
P450
(CYP450),
arachidonic
acid
metabolism,
ECM–receptor
interactions.
These
findings
suggest
predominantly
disrupts
cellular
transport
metabolic
processes.
Overall,
our
study
provides
comprehensive
insights
into
impact
canaliculata
emphasizes
importance
understanding
mechanisms
underlying
toxicity
aquatic
organisms.
Язык: Английский
Pathological and Molecular Characterization of Grass Carp Co-Infected with Two Aeromonas Species
Animals,
Год журнала:
2025,
Номер
15(2), С. 263 - 263
Опубликована: Янв. 18, 2025
The
grass
carp
(Ctenopharyngodon
idella)
is
highly
susceptible
to
infections
caused
by
Aeromonas
species,
particularly
A.
hydrophila
and
veronii.
However,
the
immunological
mechanisms
underlying
co-infection
these
pathogens
remain
largely
uncharted.
This
study
investigated
pathogenesis
host
immune
response
in
following
concurrent
infection
with
Mortality
was
observed
as
early
24
h
post-infection,
cumulative
mortality
reaching
68%.
Quantitative
analysis
demonstrated
significantly
elevated
bacterial
loads
hepatic
tissue
at
3
days
post-infection
(dpi).
Histopathological
evaluation
revealed
severe
lesions
characterized
cellular
necrosis,
cytoplasmic
vacuolization,
hemorrhagic
manifestations.
Comparative
transcriptomic
of
tissues
between
co-infected
control
specimens
identified
868
411
differentially
expressed
genes
(DEGs)
1
5
dpi,
respectively.
Gene
ontology
KEGG
pathway
analyses
significant
enrichment
immune-related
primarily
associated
Toll-like
receptor
signaling
TNF
cascades.
Notably,
metabolic
pathways
showed
substantial
suppression
while
responses
were
activated
after
infected.
These
findings
provide
novel
insights
into
host–pathogen
interactions
during
carp,
which
may
facilitate
development
effective
prevention
strategies.
Язык: Английский
Transcriptome analysis and CRISPR-Cas9-mediated mutagenesis identify gpr116 as a candidate gene for growth reduction in grass carp (Ctenopharyngodon idella)
Comparative Biochemistry and Physiology Part A Molecular & Integrative Physiology,
Год журнала:
2025,
Номер
unknown, С. 111850 - 111850
Опубликована: Март 1, 2025
Язык: Английский