Comparative Colonisation Ability of Human Faecal Microbiome Transplantation Strategies in Murine Models DOI Creative Commons
Bon‐Hee Gu,

Ho Young Jung,

Chae‐Yun Rim

и другие.

Microbial Biotechnology, Год журнала: 2025, Номер 18(6)

Опубликована: Май 30, 2025

ABSTRACT The gut microbiome plays a crucial role in maintaining intestinal homeostasis and influencing immune‐mediated diseases. Human faecal microbiota transplantation (FMT) is often employed murine models to investigate the of human microbes disease regulation, but methods for effective colonisation require refinement. This study aimed assess efficiency model using FMT with faeces, focusing particularly on impact depletion via polyethylene glycol (PEG) comparing oral‐gastric gavage enema administration routes. Our findings revealed that PEG‐induced enhanced mice. Oral‐gastric prolonged colonisation, while facilitated quicker resolution dysbiosis, both inducing selective microbial time‐dependent manner. Notably, genera such as Bacteroides , Blautia Medicaternibacter Bifidobacteria were successfully colonised, whereas Roseburia Anaerostipes Anaerobutyricum Faecalibacterium failed establish post‐FMT. These highlight challenges replicating underscore importance selecting appropriate based desired outcomes. provides valuable insights into dynamics mice, contributing development more strategies treatment.

Язык: Английский

The role of the microbiome in liver disease DOI
David Schöler, Bernd Schnabl

Current Opinion in Gastroenterology, Год журнала: 2024, Номер 40(3), С. 134 - 142

Опубликована: Фев. 16, 2024

Purpose of review The intestinal microbiome and the gut-liver axis play a major role in health disease. human gut harbors trillions microbes disruption homeostasis can contribute to liver In this review, progress field within last 3 years is summarized, focusing on metabolic dysfunction-associated steatotic disease (MASLD), alcohol-associated (ALD), autoimmune (AILD), hepatocellular carcinoma (HCC). Recent findings Changes fecal virome fungal mycobiome have been described patients with various diseases. Several microbial derived metabolites including endogenous ethanol produced by bacteria, mechanistically linked such as MASLD. Virulence factors encoded bacteria ALD, AILD HCC. Novel therapeutic approaches focused phages, pre- postbiotics successfully used preclinical models. Fecal microbiota transplantation has effective attenuating Probiotics are safe hepatitis improve alcohol addiction. Summary gut–liver plays key pathophysiology Understanding help develop precise centered therapies.

Язык: Английский

Процитировано

7
The Progression of Microbiome Therapeutics for the Management of Gastrointestinal Diseases and Beyond DOI

Jessica R. Allegretti,

Sahil Khanna, Benjamin H. Mullish

и другие.

Gastroenterology, Год журнала: 2024, Номер 167(5), С. 885 - 902

Опубликована: Май 14, 2024

Язык: Английский

Процитировано

6
From dysbiosis to defense: harnessing the gut microbiome in HIV/SIV therapy DOI Creative Commons
Jason M. Brenchley, Sergio Serrano‐Villar

Microbiome, Год журнала: 2024, Номер 12(1)

Опубликована: Июнь 21, 2024

Abstract Background Although the microbiota has been extensively associated with HIV pathogenesis, majority of studies, particularly those using omics techniques, are largely correlative and serve primarily as a basis for hypothesis generation. Furthermore, most have focused on characterizing taxonomic composition bacterial component, often overlooking other levels microbiome. The intricate mechanisms by which influences immune responses to still poorly understood. Interventional studies gut provide powerful tool test whether we can harness improve health outcomes in people HIV. Results Here, review multifaceted role microbiome HIV/SIV disease progression its potential therapeutic target. We explore complex interplay between microbial dysbiosis systemic inflammation, highlighting microbiome-based therapeutics open new avenues management. These include exploring efficacy probiotics, prebiotics, fecal transplantation, targeted dietary modifications. also address challenges inherent this research area, such difficulty inducing long-lasting alterations complexities study designs, including variations probiotic strains, donor selection FMT, antibiotic conditioning regimens, hurdles translating findings into clinical practice. Finally, speculate future directions rapidly evolving field, emphasizing need more granular understanding microbiome-immune interactions, development personalized therapies, application novel technologies identify agents. Conclusions Our underscores importance target innovative strategies.

Язык: Английский

Процитировано

5
Bile acids impact the microbiota, host, and C. difficile dynamics providing insight into mechanisms of efficacy of FMTs and microbiota-focused therapeutics DOI Creative Commons
Arthur S. McMillan, Casey M. Theriot

Gut Microbes, Год журнала: 2024, Номер 16(1)

Опубликована: Сен. 3, 2024

Clostridioides difficile is a major nosocomial pathogen, causing significant morbidity and mortality worldwide. Antibiotic usage, risk factor for infection (CDI), disrupts the gut microbiota, allowing C. to proliferate cause infection, can often lead recurrent CDI (rCDI). Fecal microbiota transplantation (FMT) live biotherapeutic products (LBPs) have emerged as effective treatments rCDI aim restore colonization resistance provided by healthy microbiota. However, much still unknown about mechanisms mediating their success. Bile acids, extensively modified microbes, affect difficile's germination, growth, toxin production while also shaping influencing host immune responses. Additionally, microbial interactions, such nutrient competition cross-feeding, contribute against may success of microbiota-focused therapeutics. acids well other mediated interactions could implications diseases being treated with This review focuses on intricate interplay between bile acid modifications, ecology, responses focus difficile, hoping shed light how move forward development new therapeutic strategies combat intestinal diseases.

Язык: Английский

Процитировано

5
Metagenomic, metabolomic, and lipidomic shifts associated with fecal microbiota transplantation for recurrent Clostridioides difficile infection DOI Creative Commons
Arthur S. McMillan,

Guozhi Zhang,

Michael Dougherty

и другие.

mSphere, Год журнала: 2024, Номер 9(10)

Опубликована: Окт. 8, 2024

ABSTRACT Recurrent C. difficile infection (rCDI) is an urgent public health threat, for which the last resort and lifesaving treatment a fecal microbiota transplant (FMT). However, exact mechanisms that mediate successful FMT are not well-understood. Here, we use longitudinal stool samples collected from patients undergoing to evaluate intra-individual changes in microbiome, metabolome, lipidome after FMTs relative their baselines pre-FMT. We show abundance of many lipids, specifically decrease acylcarnitines post-FMT, shift conjugated bile acids pre-FMT deconjugated secondary post-FMT. These correlate with Enterobacteriaceae, encode carnitine metabolism genes, increase Lachnospiraceae, acid altering genes such as salt hydrolases (BSHs) acid-inducible ( bai ) operon, also gut microbe-encoded amino biosynthesis Enterobacteriaceae was primary contributor auxotrophic for. Liquid chromatography, ion mobility spectrometry, mass spectrometry (LC-IMS-MS) revealed microbial conjugation define structural functional associated generate hypotheses require further experimental validation. This information meant help guide development new microbiota-focused therapeutics treat rCDI. IMPORTANCE transplant. acids, response FMT. pre-FMT, encodes Lachnospiraceae baiA genes. There FMT, hope will aid

Язык: Английский

Процитировано

4
Phages modulate bacterial communities in the human gut following fecal microbiota transplantation DOI Creative Commons

Michele Zuppi,

Tommi Vatanen, Brooke C. Wilson

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Янв. 25, 2024

Abstract Background: Fecal microbiota transplantation (FMT) is a therapeutic intervention used to treat diseases associated with the gut microbiome. In human microbiome, phages have been implicated in influencing health, successful engraftment of donor correlated FMT treatment efficacy. The impact that gastrointestinal exert on health has primarily connected their ability modulate bacterial communities gut. Nonetheless, how affects recipients’ phage populations, and turn, this influences environment, not yet fully understood. study, we investigated effects phageome composition participants within Gut Bugs Trial (GBT), double-blind, randomized, placebo-controlled trial efficacy treating obesity comorbidities adolescents. Stool samples collected from donors at time recipients four points ( i.e., baseline 6 weeks, 12 26 weeks post intervention), underwent shotgun metagenomic sequencing. Phage sequences were identified characterized silico examine evidence assess extent FMT-induced alterations composition. Results: Donor engrafted stably following FMT, composing significant proportion for entire course study (33.8 ± 1.2% females 33.9 3.7% males). varied between was positively alpha diversity. caused shift toward donors’ increased diversity variability over time. Conclusions: significantly altered recipients' and, overall, microbial populations. increase consistent population dynamics. This proposes play critical role modulating environment suggests novel approaches understand altering recipient's registration : registered Australian New Zealand Clinical Trials Registry (ACTR N12615001351505). protocol available https://bmjopen.bmj.com/content/9/4/e026174.

Язык: Английский

Процитировано

3
The protective role of commensal gut microbes and their metabolites against bacterial pathogens DOI Creative Commons
Liqin Cheng, Mário S. P. Correia, Shawn M. Higdon

и другие.

Gut Microbes, Год журнала: 2024, Номер 16(1)

Опубликована: Май 26, 2024

Multidrug-resistant microorganisms have become a major public health concern around the world. The gut microbiome is gold mine for bioactive compounds that protect human body from pathogens. We used multi-omics approach integrated whole-genome sequencing (WGS) of 74 commensal isolates with metabolome analysis to discover their metabolic interaction Salmonella and other antibiotic-resistant evaluated differences in functional potential these selected based on WGS annotation profiles. Furthermore, top altered metabolites co-culture supernatants were identified including series dipeptides examined ability prevent growth various bacteria. Our results provide compelling evidence produces metabolites, compound class can potentially be applied anti-infection medication, especially against established pipeline discovery validation as novel candidates multidrug-resistant infections represents new avenue antimicrobial lead structures.

Язык: Английский

Процитировано

3
Fecal Microbiota Transplantation: Indications, Methods, and Challenges DOI
Jee Young Lee,

Y. H. Kim,

Jiyoun Kim

и другие.

The Journal of Microbiology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 18, 2024

Язык: Английский

Процитировано

3
Modulation of Efferocytosis and Inflammation Resolution by Live and Non-Live Probiotics through Gut Microbiota Interactions in Preclinical and Clinical Studies DOI

Mona Arianezhad,

Farnaz Tajeddini,

Mina Zareardalan

и другие.

Food Bioscience, Год журнала: 2024, Номер unknown, С. 105498 - 105498

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

3
Effects of gastrointestinal symptoms on the efficacy of washed microbiota transplantation in patients with autism DOI Creative Commons

Dongxia Hu,

Cheng‐Kai Lu,

X.C. Si

и другие.

Frontiers in Pediatrics, Год журнала: 2025, Номер 13

Опубликована: Фев. 13, 2025

Objective Washed microbiota transplantation (WMT) has emerged as a promising therapeutic strategy for autism spectrum disorder (ASD), though the factors that influence its efficacy remain poorly understood. This study explores impact of gastrointestinal (GI) symptoms on effectiveness WMT in ASD. Methods Clinical data encompassing ASD symptoms, GI disturbances, and sleep disorders were collected from patients with undergoing WMT. The contributing to assessed. Results significantly reduced scores Aberrant Behavior Checklist (ABC), Childhood Autism Rating Scale (CARS), Sleep Disturbance Children (SDSC), alongside significant reduction incidence constipation, abnormal stool forms, diarrhea (all p < 0.05). After six courses WMT, substantial reductions observed ABC, CARS, SDSC scores, increased treatment correlating greater improvement ( Multiple linear regression analysis revealed was enhanced pre-existing (diarrhea: β = 0.119, 0.001; form: 0.201, 0.001) those receiving higher number 0.116, 0.001). Additionally, indicated outcomes more favorable who had not undergone adjunct interventions −0.041, 0.002), longer disease duration 0.168, 0.007), exhibited severe 0.125, Conclusion alleviates both along affected individuals. Six resulted notable improvement, course numbers further improving outcomes. Furthermore, pre-treatment such may Notably, did receive additional interventions, prolonged duration, presented experienced markedly improved responses.

Язык: Английский

Процитировано

0