The Journal of Microbiology, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 18, 2024
Language: Английский
Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)
Published: Sept. 3, 2024
Clostridioides difficile is a major nosocomial pathogen, causing significant morbidity and mortality worldwide. Antibiotic usage, risk factor for infection (CDI), disrupts the gut microbiota, allowing C. to proliferate cause infection, can often lead recurrent CDI (rCDI). Fecal microbiota transplantation (FMT) live biotherapeutic products (LBPs) have emerged as effective treatments rCDI aim restore colonization resistance provided by healthy microbiota. However, much still unknown about mechanisms mediating their success. Bile acids, extensively modified microbes, affect difficile's germination, growth, toxin production while also shaping influencing host immune responses. Additionally, microbial interactions, such nutrient competition cross-feeding, contribute against may success of microbiota-focused therapeutics. acids well other mediated interactions could implications diseases being treated with This review focuses on intricate interplay between bile acid modifications, ecology, responses focus difficile, hoping shed light how move forward development new therapeutic strategies combat intestinal diseases.
Language: Английский
Citations
4
Frontiers in Pediatrics, Journal Year: 2025, Volume and Issue: 13
Published: Feb. 13, 2025
Objective Washed microbiota transplantation (WMT) has emerged as a promising therapeutic strategy for autism spectrum disorder (ASD), though the factors that influence its efficacy remain poorly understood. This study explores impact of gastrointestinal (GI) symptoms on effectiveness WMT in ASD. Methods Clinical data encompassing ASD symptoms, GI disturbances, and sleep disorders were collected from patients with undergoing WMT. The contributing to assessed. Results significantly reduced scores Aberrant Behavior Checklist (ABC), Childhood Autism Rating Scale (CARS), Sleep Disturbance Children (SDSC), alongside significant reduction incidence constipation, abnormal stool forms, diarrhea (all p < 0.05). After six courses WMT, substantial reductions observed ABC, CARS, SDSC scores, increased treatment correlating greater improvement ( Multiple linear regression analysis revealed was enhanced pre-existing (diarrhea: β = 0.119, 0.001; form: 0.201, 0.001) those receiving higher number 0.116, 0.001). Additionally, indicated outcomes more favorable who had not undergone adjunct interventions −0.041, 0.002), longer disease duration 0.168, 0.007), exhibited severe 0.125, Conclusion alleviates both along affected individuals. Six resulted notable improvement, course numbers further improving outcomes. Furthermore, pre-treatment such may Notably, did receive additional interventions, prolonged duration, presented experienced markedly improved responses.
Language: Английский
Citations
0
Journal of Psychiatric Research, Journal Year: 2025, Volume and Issue: 184, P. 27 - 38
Published: Feb. 21, 2025
Language: Английский
Citations
0
Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12
Published: March 17, 2025
The human gut microbiota, an intricate ecosystem of bacteria, viruses, fungi, and archaea, plays a pivotal role in maintaining gastrointestinal (GI) homeostasis (1). Recent advancements microbiome research have unveiled its profound influence on various GI disorders, including gastric cancer, inflammatory bowel disease (IBD), irritable syndrome (IBS), acute pancreatitis, Clostridioides difficile infection (CDI) (2). Understanding the interplay between microbiota these disorders offers promising avenues for novel therapeutic interventions, next-generation probiotics, fecal transplantation (FMT), targeted microbiome-oriented modulation (3,4).Gastric cancer remains significant global health burden, ranking as fourth leading cause cancer-related mortality. Dysbiosis, characterized by altered microbial diversity composition, is hallmark carcinogenesis (Marashi et al.). Helicobacter pylori wellestablished risk factor, yet eradication alone does not fully restore equilibrium. Studies suggest that certain bacterial species, such Lactobacillus, enhance immunotherapy responses, whereas antibiotic-induced dysbiosis can reduce efficacy chemotherapy al.) (5,6). Probiotic supplementation post-gastrectomy has demonstrated potential mitigating inflammation fostering healthier milieu (7). IBD, encompassing Crohn's ulcerative colitis, immune-mediated disorder driven complex genetic, environmental, factors (Ning (8). Dysbiosis relationship metabolic functions extends beyond tract. Emerging explored bidirectional connection insulin-like growth factor 1 (IGF-1), key regulator growth-related pathways (Zheng been implicated modulating IGF-1 levels, suggesting consequences imbalances. This insight broadens scope microbiome-targeted therapies to include alongside diseases.The expanding knowledge underscores need precision medicine approaches. Personalized profiling could guide tailored optimizing outcomes. Probiotics, prebiotics, synbiotics, nextgeneration microbiome-based therapies, engineered consortia postbiotics, offer exciting prospects. Moreover, metagenomics metabolomics will further elucidate host-microbe interactions, paving way strategies (13).In conclusion, published studies this Research Topic highlight integral determinant disease. From IBS multifaceted (Stange Harnessing interventions holds immense revolutionizing management, shifting from symptomatic treatment root-cause (14). As continues unravel complexities future gastroenterology lies leveraging microscopic powerhouse transformative healthcare solutions.
Language: Английский
Citations
0
Published: Jan. 1, 2025
Language: Английский
Citations
0
MedComm, Journal Year: 2025, Volume and Issue: 6(5)
Published: April 18, 2025
ABSTRACT Dysbiosis refers to the disruption of gut microbiota balance and is pathological basis various diseases. The main pathogenic mechanisms include impaired intestinal mucosal barrier function, inflammation activation, immune dysregulation, metabolic abnormalities. These involve dysfunctions in gut–brain axis, gut–liver others cause broader effects. Although association between diseases caused by dysbiosis has been extensively studied, many questions remain regarding specific treatment strategies. This review begins examining causes summarizes potential representative imbalance. It integrates clinical evidence explore preventive therapeutic strategies targeting emphasizing importance understanding dysbiosis. Finally, we summarized development artificial intelligence (AI) research suggested that it will play a critical role future studies on combining multiomics technologies AI further uncover complex drive personalized
Language: Английский
Citations
0
Toxins, Journal Year: 2025, Volume and Issue: 17(5), P. 222 - 222
Published: May 1, 2025
Clostridioides difficile (C. difficile) is a major pathogen responsible for antibiotic-associated diarrhea, frequently observed in hospital settings. Due to the widespread use of antibiotics, incidence and severity C. infection (CDI) are rising across world. CDI primarily driven by two homologous protein exotoxins, toxin A (TcdA) B (TcdB). Other putative virulence factors include binary CDT, surface layer proteins, phosphorylated polysaccharides, spore coat proteins. These potential targets vaccine development. Although several vaccines have entered clinical trials, there currently no approved on market. This review outlines intoxication mechanism during CDI, emphasizing antigens that can be used We aim provide comprehensive overview current status research development vaccines.
Language: Английский
Citations
0
Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 25, 2024
Abstract Background: Fecal microbiota transplantation (FMT) is a therapeutic intervention used to treat diseases associated with the gut microbiome. In human microbiome, phages have been implicated in influencing health, successful engraftment of donor correlated FMT treatment efficacy. The impact that gastrointestinal exert on health has primarily connected their ability modulate bacterial communities gut. Nonetheless, how affects recipients’ phage populations, and turn, this influences environment, not yet fully understood. study, we investigated effects phageome composition participants within Gut Bugs Trial (GBT), double-blind, randomized, placebo-controlled trial efficacy treating obesity comorbidities adolescents. Stool samples collected from donors at time recipients four points ( i.e., baseline 6 weeks, 12 26 weeks post intervention), underwent shotgun metagenomic sequencing. Phage sequences were identified characterized silico examine evidence assess extent FMT-induced alterations composition. Results: Donor engrafted stably following FMT, composing significant proportion for entire course study (33.8 ± 1.2% females 33.9 3.7% males). varied between was positively alpha diversity. caused shift toward donors’ increased diversity variability over time. Conclusions: significantly altered recipients' and, overall, microbial populations. increase consistent population dynamics. This proposes play critical role modulating environment suggests novel approaches understand altering recipient's registration : registered Australian New Zealand Clinical Trials Registry (ACTR N12615001351505). protocol available https://bmjopen.bmj.com/content/9/4/e026174.
Language: Английский
Citations
3
Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)
Published: May 26, 2024
Multidrug-resistant microorganisms have become a major public health concern around the world. The gut microbiome is gold mine for bioactive compounds that protect human body from pathogens. We used multi-omics approach integrated whole-genome sequencing (WGS) of 74 commensal isolates with metabolome analysis to discover their metabolic interaction Salmonella and other antibiotic-resistant evaluated differences in functional potential these selected based on WGS annotation profiles. Furthermore, top altered metabolites co-culture supernatants were identified including series dipeptides examined ability prevent growth various bacteria. Our results provide compelling evidence produces metabolites, compound class can potentially be applied anti-infection medication, especially against established pipeline discovery validation as novel candidates multidrug-resistant infections represents new avenue antimicrobial lead structures.
Language: Английский
Citations
3
mSphere, Journal Year: 2024, Volume and Issue: 9(10)
Published: Oct. 8, 2024
ABSTRACT Recurrent C. difficile infection (rCDI) is an urgent public health threat, for which the last resort and lifesaving treatment a fecal microbiota transplant (FMT). However, exact mechanisms that mediate successful FMT are not well-understood. Here, we use longitudinal stool samples collected from patients undergoing to evaluate intra-individual changes in microbiome, metabolome, lipidome after FMTs relative their baselines pre-FMT. We show abundance of many lipids, specifically decrease acylcarnitines post-FMT, shift conjugated bile acids pre-FMT deconjugated secondary post-FMT. These correlate with Enterobacteriaceae, encode carnitine metabolism genes, increase Lachnospiraceae, acid altering genes such as salt hydrolases (BSHs) acid-inducible ( bai ) operon, also gut microbe-encoded amino biosynthesis Enterobacteriaceae was primary contributor auxotrophic for. Liquid chromatography, ion mobility spectrometry, mass spectrometry (LC-IMS-MS) revealed microbial conjugation define structural functional associated generate hypotheses require further experimental validation. This information meant help guide development new microbiota-focused therapeutics treat rCDI. IMPORTANCE transplant. acids, response FMT. pre-FMT, encodes Lachnospiraceae baiA genes. There FMT, hope will aid
Language: Английский
Citations
3