RNA communication between organisms inspires innovative eco-friendly strategies for disease control

Nature Reviews Molecular Cell Biology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 15, 2024

Язык: Английский

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Cross kingdom application of noncoding RNA

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 295 - 317

Опубликована: Янв. 1, 2025

Язык: Английский

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Fungal small RNA hijacking: a new layer of cross‐kingdom communications in arbuscular mycorrhizal symbiosis

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

… AM fungal small RNAs just entered the 'chat', and a new layer of cross-kingdom molecular signals enables symbiosis. Eukaryotic sRNAs are short noncoding regulatory elements (usually 20–25 nucleotides in length) that trigger RNAi process cells act as big players microbe–plant interactions (Huang et al., 2019). Arbuscular mycorrhizal emerging crucial molecules symbiotic networks at RNA level (Silvestri 2019; Ledford 2024). Recently, ckRNAi was revealed to form an essential component bidirectional between fungi host plants, regulating crosstalk symbiosis immunity, indicating sRNA translocation occurs (Qiao 2023). Furthermore, it has been observed arbuscular cell invasion coincides with formation extracellular vesicles (EVs) membrane tubules (Roth These findings interesting, EVs have shown represent transfer pathways for (such double-strand (dsRNAs), sRNAs, mRNAs, proteins) during plant–pathogen (Cai 2018; He 2023; Wang 2024; Zhang In addition, host- virus-induced gene silencing approaches suitable tools genes roots, supporting movement from root (Helber 2011; Kikuchi 2016). fungi, potential role fungus-to-plant processes remained elusive until Silvestri al. (2019) fungus R. irregularis possesses machinery generates many some which were predicted target mRNAs M. truncatula. also proposed possibly participate symbiosis, similar pathogen–plant interface. this study, (2024) build on their previous work by conducting silico prediction analysis identify its plant Through multiple assays, they achieved first experimental evidence mediates through (Fig. 1a–d), results promotion field biology, provides valuable insight into fungus–plant communication inspires further research mechanisms underlying symbionts. The authors use sequence complementarity approach show Rir2216 is mRNA WRKY69 Heterologous co-expression, 5′ RACE reactions, AGO1-immunoprecipitation assays demonstrate direct interaction MtWRKY69. sends sRNA, Rir2216, Medicago hijacking MtAGO1 protein-equipped silence MtWRKY69 post-transcriptional 1d). implications finding exciting. As Nasfi reported, beneficial Serendipita indica SisRNAs translocated Arabidopsis hijack AtAGO1 induce machinery, suggesting model conserved interaction. genetic manipulation hampered fact obligate biotrophs multi-nuclei. Therefore, impossible directly knockout genome present time. However, both constitutive conditional expression strategies overexpress overexpression resulted reduced mycorrhiza formation. very timely. recent review, describe 'the characteristics fungal-derived functional two partners mutually modulate control symbiosis'. Together, provide previously undescribed roots increase colonization levels. A working would be Rir2216-mediated knockdown arbuscule-containing could contribute suppression immunity enabling since WRKY transcription factors expressed response pathogens (Jiang 2017). al.'s rigorous dissection pathway better methodological framework addressing mechanistic issues. This can used validate involvement miRNAs report, highlight key roles Understanding trafficking will help us develop novel effectively promoting nutrition. Despite breakthrough, mysteries remain. Primarily, whether vitro synthetic artificially increases miRNA via treatment (Wang 2016), leading accelerated degradation transcripts. Further investigations delivers other needed Fig. Additionally, precise functions largely unknown so far; indeed, interesting create CRISPR mutant lines order elucidate loss function efficiently promotes Finally, remains 1d), extensively reported summary, publication demonstrates regulate promote sheds light how evolved colonize roots. Moreover, open avenues future. like thank Zoe Irwin, Holly Slater, Francis Martin providing feedback manuscript. grateful Junliang assembly 1. supported National Natural Science Foundation China (nos.: 32370108 32170116). New Phytologist neutral regard jurisdictional claims maps any institutional affiliations.

Язык: Английский

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Des ARN messagers… entre cellules

Pour la Science, Год журнала: 2025, Номер nº569(3), С. 32 - 36

Опубликована: Фев. 26, 2025

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KatB, a bacterial extracellular vesicles (EVs)‐secreted catalase, detoxifies reactive oxygen species (ROS) and promotes pathogen proliferation in plants

Journal of Integrative Plant Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

ABSTRACT Gram‐negative bacteria are known to release extracellular vesicles (EVs) into their surrounding environment. However, the biological functions of proteins contained within these remain largely unknown. Here, we used tandem mass tag (TMT) proteomic analysis characterize protein cargoes EVs phytopathogen Pseudomonas syringae pv. tomato DC3000 ( Pto DC3000). Our investigation revealed that one catalase, KatB, is enriched in bacterial EVs. This enzyme confers with capacity detoxify both exogenous and plant‐produced H 2 O , thereby contributing pathogen's proliferation plants. Interestingly, reactive oxygen species (ROS) stress stimulates EV secretion enhances package KatB vesicles. regulatory process depends on a periplasmic ankyrin‐like protein, AnkB. Both AnkB encoded small operon, mutant strains exhibit impaired growth plant hosts. Furthermore, treatment pelleted from culture supernatants activates immune responses plants, absence further this protective activity. Collectively, our findings indicate secreted via interact reduce host's oxidative environment, promoting

Язык: Английский

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Application of plant-derived extracellular vesicles as novel carriers in drug delivery systems: A review

Expert Opinion on Drug Delivery, Год журнала: 2025, Номер unknown

Опубликована: Март 30, 2025

Introduction Plant-derived extracellular vesicles (P-EVs) are nanoscale, lipid bilayer capable of transporting diverse bioactive substances, enabling intercellular and interspecies communication material transfer. With inherent pharmacological effects, targeting abilities, high safety, biocompatibility, low production costs, P-EVs promising candidates for drug delivery systems, offering significant application potential.

Язык: Английский

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Faecalibacterium prausnitzii-derived outer membrane vesicles reprogram gut microbiota metabolism to alleviate Porcine Epidemic Diarrhea Virus infection

Microbiome, Год журнала: 2025, Номер 13(1)

Опубликована: Апрель 2, 2025

The Porcine Epidemic Diarrhea Virus (PEDV) is one of the major challenges facing global pig farming industry, and vaccines treatments have proven difficult in controlling its spread. Faecalibacterium prausnitzii (F.prausnitzii), a key commensal bacterium gut, has been recognized as promising candidate for next-generation probiotics due to potential wide-ranging health benefits. A decrease F.prausnitzii abundance associated with certain viral infections, suggesting application preventing intestinal infections. In this study, we utilized piglet model examine role PEDV infection was established supplemented F.prausnitzii, revealing that mitigated infection. Further studies found outer membrane vesicles (OMVs) are main functional components proteomics, untargeted metabolomics, small RNA-seq were used analyze composition OMVs. Exhaustion gut microbiota demonstrated function Fp. OMVs relies on presence microbiota. Additionally, metagenomic analysis indicated altered composition, enhancing prausnitzii, Prevotellamassilia timonensis, Limosilactobacillus reuteri. Untargeted metabolomics showed increased phosphatidylcholine (PC) levels, PC identified metabolite alleviating Single-cell sequencing revealed relative cells, number epithelial reduced necroptosis target cells. treatment infected IPEC-J2 Vero cells alleviated activation RIPK1-RIPK3-MLKL signaling axis, thereby improving play critical mitigating These findings provide strategy ameliorate piglets. Video Abstract.

Язык: Английский

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The cell biology of Extracellular Vesicles: A jigsaw puzzle with a myriad of pieces

Current Opinion in Cell Biology, Год журнала: 2025, Номер 94, С. 102519 - 102519

Опубликована: Апрель 22, 2025

Язык: Английский

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Arabidopsis Produces Distinct Subpopulations of Extracellular Vesicles That Respond Differentially to Biotic Stress, Altering Growth and Infectivity of a Fungal Pathogen

Journal of Extracellular Vesicles, Год журнала: 2025, Номер 14(5)

Опубликована: Май 1, 2025

ABSTRACT Extracellular vesicles (EVs) secreted by mammalian cells are highly heterogeneous in content and function. Whether this is also true for EVs plant not yet known. To address this, we used high‐resolution density gradient ultracentrifugation total internal fluorescence microscopy (TIRF‐M) to purify distinguish distinct subpopulations of Arabidopsis EVs. The EV marker protein TETRASPANIN 8 (TET8) was detected specifically medium‐density TET8 PENETRATION 1 (PEN1) were confirmed be mostly separate populations using TIRF‐M, while PEN1 co‐secreted with 3 (PEN3) much more often. Secretion marked TET8, RPM1‐INTERACTING PROTEIN 4 (RIN4) into the apoplast onto leaf surface induced phytohormones, changes temperature infection fungal pathogens. Treatment seedlings delayed progression altering germ tube development morphology. Significantly, extracellular RNAs, including miRNAs siRNAs, did co‐fractionate TET8‐labeled EVs, instead, co‐fractionated extravesicular ARGONAUTE proteins high‐density fractions. Together, these data indicate that contribute immunity but unlikely mediate cross‐kingdom RNA interference.

Язык: Английский

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Extracellular vesicle-mediated RNA warfare: A cross-kingdom battleground

Current Opinion in Plant Biology, Год журнала: 2025, Номер 86, С. 102741 - 102741

Опубликована: Июнь 2, 2025

Язык: Английский

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