State of the Art on the Role of Staphylococcus aureus Extracellular Vesicles in the Pathogenesis of Atopic Dermatitis DOI Creative Commons
Marina Passos Torrealba, Fábio Seiti Yamada Yoshikawa, Valéria Aoki

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(3), P. 531 - 531

Published: March 6, 2024

Atopic dermatitis (AD) is a chronic and relapsing inflammatory cutaneous disease. The role of host defense microbial virulence factors in Staphylococcus aureus skin colonization, infection, inflammation perpetuation AD remains an area current research focus. Extracellular vesicles (EV) mediate cell-to-cell communication by transporting delivering bioactive molecules, such as nucleic acids, proteins, enzymes, to recipient cells. spontaneously secretes extracellular (SA-derived EVs), which spread throughout the layers. Previous has shown that SA-derived EVs from patients can trigger cytokine secretion keratinocytes, shape recruitment neutrophils monocytes, induce AD-type lesions mouse models, addition their exogenous worsening for In this review article, we aim examine physiopathology its progression, highlighting recent field exploring potential crosstalk between microbiota.

Language: Английский

Cross kingdom application of noncoding RNA DOI
Chintan Kapadia, Rahul Datta, Sachidanand Singh

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 295 - 317

Published: Jan. 1, 2025

Language: Английский

Citations

0
Fungal small RNA hijacking: a new layer of cross‐kingdom communications in arbuscular mycorrhizal symbiosis DOI Open Access
Xianan Xie, Xiaoning Fan

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

… AM fungal small RNAs just entered the 'chat', and a new layer of cross-kingdom molecular signals enables symbiosis. Eukaryotic sRNAs are short noncoding regulatory elements (usually 20–25 nucleotides in length) that trigger RNAi process cells act as big players microbe–plant interactions (Huang et al., 2019). Arbuscular mycorrhizal emerging crucial molecules symbiotic networks at RNA level (Silvestri 2019; Ledford 2024). Recently, ckRNAi was revealed to form an essential component bidirectional between fungi host plants, regulating crosstalk symbiosis immunity, indicating sRNA translocation occurs (Qiao 2023). Furthermore, it has been observed arbuscular cell invasion coincides with formation extracellular vesicles (EVs) membrane tubules (Roth These findings interesting, EVs have shown represent transfer pathways for (such double-strand (dsRNAs), sRNAs, mRNAs, proteins) during plant–pathogen (Cai 2018; He 2023; Wang 2024; Zhang In addition, host- virus-induced gene silencing approaches suitable tools genes roots, supporting movement from root (Helber 2011; Kikuchi 2016). fungi, potential role fungus-to-plant processes remained elusive until Silvestri al. (2019) fungus R. irregularis possesses machinery generates many some which were predicted target mRNAs M. truncatula. also proposed possibly participate symbiosis, similar pathogen–plant interface. this study, (2024) build on their previous work by conducting silico prediction analysis identify its plant Through multiple assays, they achieved first experimental evidence mediates through (Fig. 1a–d), results promotion field biology, provides valuable insight into fungus–plant communication inspires further research mechanisms underlying symbionts. The authors use sequence complementarity approach show Rir2216 is mRNA WRKY69 Heterologous co-expression, 5′ RACE reactions, AGO1-immunoprecipitation assays demonstrate direct interaction MtWRKY69. sends sRNA, Rir2216, Medicago hijacking MtAGO1 protein-equipped silence MtWRKY69 post-transcriptional 1d). implications finding exciting. As Nasfi reported, beneficial Serendipita indica SisRNAs translocated Arabidopsis hijack AtAGO1 induce machinery, suggesting model conserved interaction. genetic manipulation hampered fact obligate biotrophs multi-nuclei. Therefore, impossible directly knockout genome present time. However, both constitutive conditional expression strategies overexpress overexpression resulted reduced mycorrhiza formation. very timely. recent review, describe 'the characteristics fungal-derived functional two partners mutually modulate control symbiosis'. Together, provide previously undescribed roots increase colonization levels. A working would be Rir2216-mediated knockdown arbuscule-containing could contribute suppression immunity enabling since WRKY transcription factors expressed response pathogens (Jiang 2017). al.'s rigorous dissection pathway better methodological framework addressing mechanistic issues. This can used validate involvement miRNAs report, highlight key roles Understanding trafficking will help us develop novel effectively promoting nutrition. Despite breakthrough, mysteries remain. Primarily, whether vitro synthetic artificially increases miRNA via treatment (Wang 2016), leading accelerated degradation transcripts. Further investigations delivers other needed Fig. Additionally, precise functions largely unknown so far; indeed, interesting create CRISPR mutant lines order elucidate loss function efficiently promotes Finally, remains 1d), extensively reported summary, publication demonstrates regulate promote sheds light how evolved colonize roots. Moreover, open avenues future. like thank Zoe Irwin, Holly Slater, Francis Martin providing feedback manuscript. grateful Junliang assembly 1. supported National Natural Science Foundation China (nos.: 32370108 32170116). New Phytologist neutral regard jurisdictional claims maps any institutional affiliations.

Language: Английский

Citations

0
Des ARN messagers… entre cellules DOI

Stéphanie Melchor

Pour la Science, Journal Year: 2025, Volume and Issue: nº569(3), P. 32 - 36

Published: Feb. 26, 2025

Citations

0
KatB, a bacterial extracellular vesicles (EVs)‐secreted catalase, detoxifies reactive oxygen species (ROS) and promotes pathogen proliferation in plants DOI Open Access

Jiliang Deng,

Wei Li,

Zhangying Wang

et al.

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

ABSTRACT Gram‐negative bacteria are known to release extracellular vesicles (EVs) into their surrounding environment. However, the biological functions of proteins contained within these remain largely unknown. Here, we used tandem mass tag (TMT) proteomic analysis characterize protein cargoes EVs phytopathogen Pseudomonas syringae pv. tomato DC3000 ( Pto DC3000). Our investigation revealed that one catalase, KatB, is enriched in bacterial EVs. This enzyme confers with capacity detoxify both exogenous and plant‐produced H 2 O , thereby contributing pathogen's proliferation plants. Interestingly, reactive oxygen species (ROS) stress stimulates EV secretion enhances package KatB vesicles. regulatory process depends on a periplasmic ankyrin‐like protein, AnkB. Both AnkB encoded small operon, mutant strains exhibit impaired growth plant hosts. Furthermore, treatment pelleted from culture supernatants activates immune responses plants, absence further this protective activity. Collectively, our findings indicate secreted via interact reduce host's oxidative environment, promoting

Language: Английский

Citations

0
Application of plant-derived extracellular vesicles as novel carriers in drug delivery systems: A review DOI
Jia-Yi Yang, Xinyi Ai, Chenming Zhang

et al.

Expert Opinion on Drug Delivery, Journal Year: 2025, Volume and Issue: unknown

Published: March 30, 2025

Introduction Plant-derived extracellular vesicles (P-EVs) are nanoscale, lipid bilayer capable of transporting diverse bioactive substances, enabling intercellular and interspecies communication material transfer. With inherent pharmacological effects, targeting abilities, high safety, biocompatibility, low production costs, P-EVs promising candidates for drug delivery systems, offering significant application potential.

Language: Английский

Citations

0
Faecalibacterium prausnitzii-derived outer membrane vesicles reprogram gut microbiota metabolism to alleviate Porcine Epidemic Diarrhea Virus infection DOI Creative Commons

Jun-Hong Xing,

Tianming Niu,

Tong Yu

et al.

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 2, 2025

The Porcine Epidemic Diarrhea Virus (PEDV) is one of the major challenges facing global pig farming industry, and vaccines treatments have proven difficult in controlling its spread. Faecalibacterium prausnitzii (F.prausnitzii), a key commensal bacterium gut, has been recognized as promising candidate for next-generation probiotics due to potential wide-ranging health benefits. A decrease F.prausnitzii abundance associated with certain viral infections, suggesting application preventing intestinal infections. In this study, we utilized piglet model examine role PEDV infection was established supplemented F.prausnitzii, revealing that mitigated infection. Further studies found outer membrane vesicles (OMVs) are main functional components proteomics, untargeted metabolomics, small RNA-seq were used analyze composition OMVs. Exhaustion gut microbiota demonstrated function Fp. OMVs relies on presence microbiota. Additionally, metagenomic analysis indicated altered composition, enhancing prausnitzii, Prevotellamassilia timonensis, Limosilactobacillus reuteri. Untargeted metabolomics showed increased phosphatidylcholine (PC) levels, PC identified metabolite alleviating Single-cell sequencing revealed relative cells, number epithelial reduced necroptosis target cells. treatment infected IPEC-J2 Vero cells alleviated activation RIPK1-RIPK3-MLKL signaling axis, thereby improving play critical mitigating These findings provide strategy ameliorate piglets. Video Abstract.

Language: Английский

Citations

0
The cell biology of Extracellular Vesicles: A jigsaw puzzle with a myriad of pieces DOI
Gisela D’Angelo,

Philip D. Stahl,

Graça Raposo

et al.

Current Opinion in Cell Biology, Journal Year: 2025, Volume and Issue: 94, P. 102519 - 102519

Published: April 22, 2025

Language: Английский

Citations

0
Tracking the messengers: Emerging advances in mRNA-based plant communication DOI
Saikat Paul, David Jackson, Munenori Kitagawa

et al.

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 79, P. 102541 - 102541

Published: April 25, 2024

Language: Английский

Citations

2
Extracellular Vesicles in Pathogenic Infection, Transmission, and Immunity DOI Creative Commons

Junyao Xiong,

Usama Ashraf, Jing Ye

et al.

Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Extracellular vesicles (EVs) are secreted by cells and widely exist in body fluids, serving as an essential vehicle of intercellular communication. In recent years, EVs have gained significant attention owing to their ability carry nucleic acids, lipids, proteins host pathogen origins. A distinct composition during pathogenic infection contributes mediating infection, transmission, immunity target cells. Therefore, studying the role is crucial for understanding pathogen-host interactions developing new anti-pathogenic therapies. This review offers overview current knowledge context pathogens. Harnessing EVs' biology may lay a foundation mitigation associated disease outcomes.

Language: Английский

Citations

2
Cross-Kingdom RNA Transport Based on Extracellular Vesicles Provides Innovative Tools for Plant Protection DOI Creative Commons

Yujin Zhao,

Yanguang Zhou,

Jingyan Xu

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(19), P. 2712 - 2712

Published: Sept. 27, 2024

RNA interference (RNAi) shows great potential in plant defense against pathogens through RNA-mediated sequence-specific gene silencing. Among RNAi-based protection strategies, spray-induced silencing (SIGS) is considered a more promising approach because it utilizes the transfer of exogenous between plants and microbes to silence target pathogen genes. The application nanovesicles significantly enhances stability delivery efficiency, thereby improving effectiveness SIGS further enhancing resistance diseases pathogens. This review explores role RNAi protection, focusing on cross-kingdom transport small RNAs (sRNAs) via extracellular vesicles. It also nanotechnology optimize RNA-based offering innovative tools methods modern biotechnology.

Language: Английский

Citations

2