Rapid screening and isolation of potential xanthine oxidase inhibitors in Ganoderma lucidum DOI Creative Commons

Wanchao Hou,

Xu Zhou, Siyuan Zhuang

и другие.

Arabian Journal of Chemistry, Год журнала: 2024, Номер 17(9), С. 105925 - 105925

Опубликована: Июль 22, 2024

Given the abundance and intricate nature of chemical components found in natural food ingredients, functional research has long grappled with two significant technical challenges: rapid precise screening active substances targeted preparation. To enhance efficiency fungal preparation, novel methodologies for production xanthine oxidase inhibitors from G. lucidum have been developed. The were rapidly screened using AUF-LC-MS. Molecular docking molecular dynamics simulation used to verify anti-gout activity compounds. Subsequently, as guide, HSCCC SPLC employed separate ingredients. Simultaneously, mechanism action was analyzed by enzymatic reaction kinetics. Finally, verified network pharmacology, its discussed. Four potential inhibitors, namely ganoderic acid C2, B, A, D2, lucidum. Their binding energies −6.29, −6.65, −4.29, −5.96 kcal/mol, respectively. purity four triterpenoid ingredients isolated more than 90 %. These showed a reversible inhibitory effect on oxidase. Network pharmacological studies revealed 71 common targets gout, obtained intersecting targets, identified main metabolic pathways. results indicate that extract can be prevention treatment gout disease. We compounds preparation incorporating enzyme inhibition assays computerized virtual compound chromatography. With this approach, we seamlessly incorporate fast discovery, screening, focused well delving into mechanisms monomers proteases, molecular-level interactions between proteases. This study providing opportunities discover develop new therapeutics health resources.

Язык: Английский

Monitoring and Managing Endocrine Disrupter Pesticides (EPDS) for Environmental Sustainability DOI

Vivek Chintada,

K. Veraiah,

Narasimha Golla

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Direct Corona Charging: A New Strategy for Enhancing Sensitivity and Stability in Charged Aerosol Detection DOI
Su Feng, Hongyi Liu,

Cong Zhou

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

The charged aerosol detector (CAD) is widely used for detecting nonvolatile compounds without UV absorption due to its high sensitivity, stability, and consistent response. However, the traditional plasma collision charging mode (PCCM) suffers from issues such as sample dilution, gas flow back-mixing, disturbances, which reduce sensitivity repeatability. To address these limitations, this study introduces direct corona (DCCM), eliminates route by using a high-voltage needle directly charge dried aerosol. This approach avoids mixing collisions, simplifies instrument structure, reduces nitrogen consumption. Comparative analyses samples like caffeine demonstrated that DCCM significantly improves stability over PCCM. response under fits quadratic curve with correlation coefficient above 0.99 across 3 orders of magnitude. Direct injection 22 analytes showed achieved peak area relative standard deviation below 10%, better consistency than Gradient analysis complex further confirmed DCCM's superior repeatability sensitivity. Real-sample highlighted DCCM-CAD's practical potential. In summary, enhances CAD performance, costs, design, paving way more efficient reliable commercial systems.

Язык: Английский

Процитировано

0

Phytochemistry, quality control and biosynthesis in ginseng research from 2021 to 2023: A state-of-the-art review concerning advances and challenges DOI Open Access

Mengxiang Ding,

Huizhen Cheng,

Xiaohang Li

и другие.

Chinese Herbal Medicines, Год журнала: 2024, Номер 16(4), С. 505 - 520

Опубликована: Авг. 23, 2024

L. (Araliaceae) has a long history of medicinal and edible use due to its significant tonifying effects, ginseng research been hot topic in natural products food science. In continuation our recent review, we highlighted the advances from 2021 2023 with 157 citations, which exhibited increasingly systematic, collaborative, intelligent characteristics. this firstly updated progress phytochemistry involving ginsenosides polysaccharides summarized researches on active components. Then, some specific applications by feat multidimensional chromatography, mass spectrometry imaging, DNA barcoding, metabolomics, were analyzed, could provide rich information supporting multi-component characterization, authentication, quality control versatile products. Finally, biosynthesis studies concerning retrospected. Additionally, current challenges future trends respect discussed.

Язык: Английский

Процитировано

2

In-depth analysis of the acetylcholinesterase inhibitors of Ganoderma amboinense based receptor-ligand affinity coupled with complex chromatography DOI

Wanchao Hou,

Zhen Liu, Yuchi Zhang

и другие.

Journal of Food Composition and Analysis, Год журнала: 2023, Номер 125, С. 105776 - 105776

Опубликована: Окт. 20, 2023

Язык: Английский

Процитировано

5

Online Comprehensive Two-Dimensional Liquid Chromatography/Quadrupole Time-of-Flight Mass Spectrometry-Based Metabolic Profiling and Comparison Enabling the Characterization of 1146 Ginsenosides and More Explicit Differentiation of Ginseng DOI
Simiao Wang,

Yadan Zou,

Min Zhang

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(44), С. 24866 - 24878

Опубликована: Окт. 23, 2024

This work was designed for the in-depth characterization and holistic comparison of up to 12 ginseng varieties, which can benefit development functional foods ensure their authenticity in food industry. An online comprehensive two-dimensional liquid chromatography/quadrupole time-of-flight mass spectrometry (2D-LC/QTOF-MS) approach established by configurating XCharge C18 HSS Cyano columns. Under optimal conditions, we characterized a total 1146 ginsenosides (including 876 potentially new compounds) from varieties reference an in-house library 573 known 70 compounds. The 2D-LC/QTOF-MS-based untargeted metabolomics workflows were developed, 126 potential ginsenoside markers unveiled utilized establish key identification points each species. Compared with conventional chromatography/mass metabolomics, our multidimensional chromatography performed better discriminating multiple varieties. demonstrates potent practical methodology identify easily confused plants.

Язык: Английский

Процитировано

1

Integration of ion mobility-quadrupole time-of-flight mass spectrometry and triple quadrupole-ion trap mass spectrometry coupled with ultra-high performance liquid chromatography for characterizing and quantitatively assay the saponins of Panax quinquefolius flower DOI
Yu Wang, Lili Hong,

Mengxiao Sun

и другие.

Microchemical Journal, Год журнала: 2024, Номер 207, С. 112124 - 112124

Опубликована: Ноя. 9, 2024

Язык: Английский

Процитировано

1

Rapid screening and isolation of potential xanthine oxidase inhibitors in Ganoderma lucidum DOI Creative Commons

Wanchao Hou,

Xu Zhou, Siyuan Zhuang

и другие.

Arabian Journal of Chemistry, Год журнала: 2024, Номер 17(9), С. 105925 - 105925

Опубликована: Июль 22, 2024

Given the abundance and intricate nature of chemical components found in natural food ingredients, functional research has long grappled with two significant technical challenges: rapid precise screening active substances targeted preparation. To enhance efficiency fungal preparation, novel methodologies for production xanthine oxidase inhibitors from G. lucidum have been developed. The were rapidly screened using AUF-LC-MS. Molecular docking molecular dynamics simulation used to verify anti-gout activity compounds. Subsequently, as guide, HSCCC SPLC employed separate ingredients. Simultaneously, mechanism action was analyzed by enzymatic reaction kinetics. Finally, verified network pharmacology, its discussed. Four potential inhibitors, namely ganoderic acid C2, B, A, D2, lucidum. Their binding energies −6.29, −6.65, −4.29, −5.96 kcal/mol, respectively. purity four triterpenoid ingredients isolated more than 90 %. These showed a reversible inhibitory effect on oxidase. Network pharmacological studies revealed 71 common targets gout, obtained intersecting targets, identified main metabolic pathways. results indicate that extract can be prevention treatment gout disease. We compounds preparation incorporating enzyme inhibition assays computerized virtual compound chromatography. With this approach, we seamlessly incorporate fast discovery, screening, focused well delving into mechanisms monomers proteases, molecular-level interactions between proteases. This study providing opportunities discover develop new therapeutics health resources.

Язык: Английский

Процитировано

0