Molecular Insights into TT2-Type MYB Regulators Illuminate the Complexity of Floral Flavonoids Biosynthesis in Freesia hybrida DOI Creative Commons
Xiaotong Shan,

Deyu Zhuang,

Ruifang Gao

и другие.

Horticulture Research, Год журнала: 2024, Номер unknown

Опубликована: Дек. 12, 2024

Proanthocyanidins (PAs), anthocyanins, and flavonols are key flavonoids that play diverse roles in plant physiology human health. Despite originating from a shared biosynthetic pathway, the regulatory mechanisms of PA biosynthesis cooperative regulation different kinds remain elusive, particularly flower tissues or organs. Here, we elucidated network governing Freesia hybrida 'Red River®' by characterizing four TT2-type MYB transcription factors, designated FhMYBPAs. Phylogenetic analysis, subcellular localization, transactivation assays predicted their as PA-related activators. Pearson correlation analysis revealed significant correlations between FhMYBPAs accumulation various floral development stages. Functional studies demonstrated activated directly binding to promoters target genes, which can be enhanced FhTT8L. Additionally, hierarchical feedback model involving FhTTG1, FhMYB27, FhMYBx was proposed for biosynthesis. Furthermore, comparative flavonoid-related factors FhPAP1, FhMYB5, FhMYBF1, FhMYB21L2 highlighted regulating PA, anthocyanin, flavonol biosynthesis, with some exhibiting versatile regulations. Overall, our findings provide insights into spatio-temporal flowers expand understanding MYB-mediated transcriptional specialized metabolites plants.

Язык: Английский

No tillage with straw mulching enhanced radiation use efficiency of wheat via optimizing canopy radiation interception and photosynthetic properties DOI
Pan Li, Wen Yin,

Lianhao Zhao

и другие.

Field Crops Research, Год журнала: 2025, Номер 326, С. 109854 - 109854

Опубликована: Март 14, 2025

Язык: Английский

Процитировано

2
The Gmbhlh13-Gmchs7 Module Positively Regulates Isoflavones Accumulation in Soybean (Glycine Max. L) DOI
Tianyi Liu, Fan Yan, Yajing Liu

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Genome-wide identification and analysis of petal color related MYB transcription factor gene in Gerbera hybrida DOI Creative Commons

Desheng Kong,

Tao Jiang,

Wei Zhao

и другие.

Industrial Crops and Products, Год журнала: 2025, Номер 232, С. 121284 - 121284

Опубликована: Май 29, 2025

Язык: Английский

Процитировано

0
A single nucleotide polymorphism affects protein translation and leads to post‐anthesis color change variation in closely related Lotus species DOI
Ruifang Gao, Yueqing Li,

Xiaotong Shan

и другие.

The Plant Journal, Год журнала: 2024, Номер unknown

Опубликована: Дек. 5, 2024

SUMMARY Flower color change, a common phenomenon that is important in pollination ecology, has intrigued scientists for decades. While previous flower studies have mainly focused on diversity among different plant species, our focus unraveling the mechanism of post‐anthesis change (PACC) and molecular basis its presence absence, respectively, two closely related species Lotus , filicaulis japonicus MG20. Metabolomic analysis reveals anthocyanins as key metabolites responsible observed PACC. Differential expression anthocyanin biosynthetic transport genes causes variation PACC between species. Crucially, significant upregulation functionally characterized MYB regulator, LfPAP1 linked to accumulation visible alterations L. flowers. Notably, we uncover nucleotide polymorphism initiation codon LjPAP1 . Although this mutation does not affect transcription, show it major effect attenuating protein translation, reducing capacity activate biosynthesis, leading failure Our study sheds light mechanisms highlights potential mutations sequences generate phenotypic differences evolution.

Язык: Английский

Процитировано

2
Molecular Insights into TT2-Type MYB Regulators Illuminate the Complexity of Floral Flavonoids Biosynthesis in Freesia hybrida DOI Creative Commons
Xiaotong Shan,

Deyu Zhuang,

Ruifang Gao

и другие.

Horticulture Research, Год журнала: 2024, Номер unknown

Опубликована: Дек. 12, 2024

Proanthocyanidins (PAs), anthocyanins, and flavonols are key flavonoids that play diverse roles in plant physiology human health. Despite originating from a shared biosynthetic pathway, the regulatory mechanisms of PA biosynthesis cooperative regulation different kinds remain elusive, particularly flower tissues or organs. Here, we elucidated network governing Freesia hybrida 'Red River®' by characterizing four TT2-type MYB transcription factors, designated FhMYBPAs. Phylogenetic analysis, subcellular localization, transactivation assays predicted their as PA-related activators. Pearson correlation analysis revealed significant correlations between FhMYBPAs accumulation various floral development stages. Functional studies demonstrated activated directly binding to promoters target genes, which can be enhanced FhTT8L. Additionally, hierarchical feedback model involving FhTTG1, FhMYB27, FhMYBx was proposed for biosynthesis. Furthermore, comparative flavonoid-related factors FhPAP1, FhMYB5, FhMYBF1, FhMYB21L2 highlighted regulating PA, anthocyanin, flavonol biosynthesis, with some exhibiting versatile regulations. Overall, our findings provide insights into spatio-temporal flowers expand understanding MYB-mediated transcriptional specialized metabolites plants.

Язык: Английский

Процитировано

1