World Journal of Stem Cells,
Год журнала:
2025,
Номер
17(1)
Опубликована: Янв. 17, 2025
Mesenchymal
stem
cells
(MSCs)
are
promising
candidates
for
regenerative
therapy
due
to
their
self-renewal
capability,
multilineage
differentiation
potential,
and
immunomodulatory
effects.
The
molecular
characteristics
of
MSCs
influenced
by
location.
Recently,
epidural
fat
(EF)
EF-derived
(EF-MSCs)
have
garnered
attention
potential
benefits
the
spinal
microenvironment
high
expression
neural
SC
markers.
However,
clinical
applications
limited
cell
senescence
accessibility
EF.
Although
many
studies
attempted
establish
an
immortalized,
stable
line,
immortalized
EF-MSCs
remain
be
clarified.
To
analyze
EF-MSCs.
phenotypes
were
analyzed
using
optical
microscopy.
Cell
immortalization
was
performed
lentiviral
vectors.
biomolecular
immunoblotting,
quantitative
PCR,
proteomics.
demonstrated
a
significantly
extended
lifespan
compared
control
group,
with
well-preserved
adipogenic
surface
marker
expression.
Introduction
human
telomerase
reverse
transcriptase
genes
markedly
increased
Proteomics
analysis
revealed
substantial
increase
in
DNA
replication
pathway
components
Immortalized
exhibited
enhanced
proliferative
capacity,
retained
upregulated
components.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(24), С. 14044 - 14056
Опубликована: Июнь 7, 2024
Intramuscular
fat
(IMF)
contributed
positively
to
pork
quality,
whereas
subcutaneous
(SCF)
was
often
considered
be
a
detrimental
factor
impacting
growth
and
carcass
traits.
Reducing
SCF
while
maintaining
optimal
IMF
levels
requires
thorough
understanding
of
the
adipogenic
differences
between
these
two
adipose
depots.
Our
study
explored
in
adipogenesis
porcine
SCF,
results
showed
that
adipocytes
(SCAs)
demonstrate
greater
potential
for
differentiation,
both
vivo
vitro.
Lipidomic
transcriptomic
analyses
suggested
intramuscular
(IMAs)
are
more
inclined
biosynthesize
unsaturated
fatty
acids.
Furthermore,
single-cell
RNA
sequencing
(scRNA-seq)
employed
dissect
intrinsic
microenvironmental
discrepancies
SCF.
Comparative
analysis
indicated
enriched
with
preadipocytes,
exhibiting
an
enhanced
potential,
characterized
by
higher
abundance
stem
cells.
coculture
cells
myogenetic
niche
IMAs
inhibited
its
differentiation.
Cell
communication
identified
160
ligand–receptor
pairs
channels
IMF.
Collectively,
our
elucidated
novel
mechanisms
underpinning
divergence
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Апрель 30, 2025
Although
the
drug
therapeutic
targets
of
acute
respiratory
distress
syndrome
(ARDS)
are
still
unclear
and
no
specific
drugs
for
ARDS
treatment
have
been
found,
some
breakthroughs
gradually
made
in
biological
target
pathways
such
as
endothelial
injury.
The
Traditional
Chinese
Medicine
Systems
Pharmacology
(TCMSP)
database
suggests
that
Acetyl-11-keto-β-boswellic
acid
(AKBA),
a
processed
product
boswellic
acid,
may
be
an
effective
intervention
ARDS.
After
preliminary
vitro
vivo
verification
protective
role
AKBA
on
ARDS,
order
to
explore
mechanism
we
used
transcriptomic
proteomic
methods
its
main
targets,
molecular
docking
cell
thermal
shift
assay
(CETSA)
further
reveal
potential
value
bone
marrow
stromal
antigen
2
(BST2)
target.
We
subsequently
examined
effect
targeting
BST2
tubule
formation,
proliferation
(Colony
formation
EdU
assay),
migration
(transwell
scratch
assays),
apoptosis
autophagy
levels
both
vivo,
protein
changes
(analyzed
by
Western
blotting
analysis).
Our
results
show
unphosphorylated
signal
transducers
transcription
activation
factors
(U-STAT1)
bins
promoter
encourage
more
anchoring
human
pulmonary
microvascular
cells
(HPMECs),
thus
inhibiting
autophagy,
promoting
tube
restraining
cecal
ligation
puncture
(CLP)
induced
lung
tissue
damage
mice.
In
conclusion,
strategy
Alternatively,
contribute
HPMECs,
STAT1
factor
expression
bind
promoter.
World Journal of Stem Cells,
Год журнала:
2025,
Номер
17(1)
Опубликована: Янв. 17, 2025
Mesenchymal
stem
cells
(MSCs)
are
promising
candidates
for
regenerative
therapy
due
to
their
self-renewal
capability,
multilineage
differentiation
potential,
and
immunomodulatory
effects.
The
molecular
characteristics
of
MSCs
influenced
by
location.
Recently,
epidural
fat
(EF)
EF-derived
(EF-MSCs)
have
garnered
attention
potential
benefits
the
spinal
microenvironment
high
expression
neural
SC
markers.
However,
clinical
applications
limited
cell
senescence
accessibility
EF.
Although
many
studies
attempted
establish
an
immortalized,
stable
line,
immortalized
EF-MSCs
remain
be
clarified.
To
analyze
EF-MSCs.
phenotypes
were
analyzed
using
optical
microscopy.
Cell
immortalization
was
performed
lentiviral
vectors.
biomolecular
immunoblotting,
quantitative
PCR,
proteomics.
demonstrated
a
significantly
extended
lifespan
compared
control
group,
with
well-preserved
adipogenic
surface
marker
expression.
Introduction
human
telomerase
reverse
transcriptase
genes
markedly
increased
Proteomics
analysis
revealed
substantial
increase
in
DNA
replication
pathway
components
Immortalized
exhibited
enhanced
proliferative
capacity,
retained
upregulated
components.
