Communications Biology,
Год журнала:
2024,
Номер
7(1)
Опубликована: Сен. 16, 2024
Electromagnetic
fields
(EMFs)
have
emerged
as
an
effective
treatment
for
osteoporosis.
However,
the
specific
mechanism
underlying
their
therapeutic
efficacy
remains
controversial.
Herein,
we
confirm
pro-osteogenic
effects
of
15
Hz
and
0.4-1
mT
low-frequency
sinusoidal
EMFs
(SEMFs)
on
rat
bone
marrow
mesenchymal
stem
cells
(BMSCs).
Subsequent
miRNA
sequencing
reveal
that
miR-34b-5p
is
downregulated
in
both
0.4
1
SEMFs-stimulated
groups.
To
clarify
role
osteogenesis,
BMSCs
are
transfected
separately
with
mimic
inhibitor.
The
results
indicate
transfection
suppress
osteogenic
differentiation,
whereas
inhibition
promote
differentiation
BMSCs.
In
vivo
assessments
using
microcomputed
tomography,
H&E
staining,
Masson
staining
show
inhibitor
injections
alleviate
mass
loss
trabecular
microstructure
deterioration
ovariectomy
(OVX)
rats.
Further
validation
demonstrates
exerts
its
by
regulating
STAC2
expression.
Modulating
miR-34b-5p/STAC2
axis
attenuate
SEMFs
These
studies
effect
partly
due
to
regulation
pathway,
which
provides
a
potential
candidate
Signal Transduction and Targeted Therapy,
Год журнала:
2025,
Номер
10(1)
Опубликована: Март 7, 2025
Redox
signaling
acts
as
a
critical
mediator
in
the
dynamic
interactions
between
organisms
and
their
external
environment,
profoundly
influencing
both
onset
progression
of
various
diseases.
Under
physiological
conditions,
oxidative
free
radicals
generated
by
mitochondrial
respiratory
chain,
endoplasmic
reticulum,
NADPH
oxidases
can
be
effectively
neutralized
NRF2-mediated
antioxidant
responses.
These
responses
elevate
synthesis
superoxide
dismutase
(SOD),
catalase,
well
key
molecules
like
nicotinamide
adenine
dinucleotide
phosphate
(NADPH)
glutathione
(GSH),
thereby
maintaining
cellular
redox
homeostasis.
Disruption
this
finely
tuned
equilibrium
is
closely
linked
to
pathogenesis
wide
range
Recent
advances
have
broadened
our
understanding
molecular
mechanisms
underpinning
dysregulation,
highlighting
pivotal
roles
genomic
instability,
epigenetic
modifications,
protein
degradation,
metabolic
reprogramming.
findings
provide
foundation
for
exploring
regulation
mechanistic
basis
improving
therapeutic
strategies.
While
antioxidant-based
therapies
shown
early
promise
conditions
where
stress
plays
primary
pathological
role,
efficacy
diseases
characterized
complex,
multifactorial
etiologies
remains
controversial.
A
deeper,
context-specific
signaling,
particularly
redox-sensitive
proteins,
designing
targeted
aimed
at
re-establishing
balance.
Emerging
small
molecule
inhibitors
that
target
specific
cysteine
residues
proteins
demonstrated
promising
preclinical
outcomes,
setting
stage
forthcoming
clinical
trials.
In
review,
we
summarize
current
intricate
relationship
disease
also
discuss
how
these
insights
leveraged
optimize
strategies
practice.
Redox Biology,
Год журнала:
2025,
Номер
81, С. 103551 - 103551
Опубликована: Фев. 14, 2025
Iron
overload
and
related
oxidative
damage
are
seen
in
many
rare
diseases,
due
to
mutation
of
iron
homeostasis-related
genes.
As
a
core
regulator
on
cellular
antioxidant
reaction,
Nrf2
can
also
decrease
systemic
levels
by
regulating
iron-related
genes
pathways,
making
activators
very
good
candidates
for
the
treatment
disorders.
Successful
examples
include
clinical
use
omaveloxolone
Friedreich's
Ataxia
dimethyl
fumarate
relapsing-remitting
multiple
sclerosis.
Despite
these
uses,
therapeutic
potentials
disorders
may
be
overlooked
practice.
Therefore,
this
study
talks
about
potential
use,
possible
mechanisms,
precautions
treating
diseases.
In
addition,
combination
therapy
with
chelators
is
proposed
reference,
aiming
facilitate
more
Expert Opinion on Therapeutic Patents,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 5, 2025
Introduction
Kelch-like
ECH-associated
protein
1
(Keap1),
an
E3
ligase
negatively
regulating
the
nuclear
factor
erythroid
2-related
2
(Nrf2),
has
emerged
as
auspicious
drug
target
for
treating
ailments
associated
with
oxidative
stress
and
inflammation.
Discovery
of
Keap1
inhibitors
have
attracted
significant
interest.
Arthritis Research & Therapy,
Год журнала:
2025,
Номер
27(1)
Опубликована: Март 8, 2025
Quercetagetin,
a
flavonoid
derived
from
the
natural
herb
Flos
eriocauli,
is
used
in
traditional
Chinese
medicine
for
its
fire-purging
(anti-inflammation)
and
wind-expelling
(pain-alleviating)
properties.
However,
potential
effects
concerning
rheumatoid
arthritis
(RA)
remain
underexplored.
This
study
was
designed
to
elucidate
associations
between
Quercetagetin
RA,
establishing
therapeutic
of
related
mechanisms
RA
treatment.
Network
pharmacology
conducted
decipher
targets
signaling
pathways
RA.
In
vitro
assays
were
then
explore
on
osteoclast
cell
behaviors
corresponding
pathways.
vivo
further
validated
effect
collagen
antibody-induced
(CAIA)
mice.
The
network
pharmacological
analysis
indicated
an
intimate
correlation
with
RA-related
inflammatory
osteolysis
Pertaining
biological
validations,
2
µM
successfully
inhibited
LPS-driven
differentiation
function.
qPCR
assay
Western
blot
analyses
denoted
parallel
changes
osteoclastic
marker
genes
proteins.
Further
mechanism
uncovered
stimulating
Nrf2/Keap1
pathway
moderating
Pten/AKT/Nfatc1
axis
osteoclasts.
revealed
40
mg/kg
every
day
could
significantly
relief
joint
destruction
CAIA
Our
presents
's
treating
outlining
suppressing
LPS-induced
activity,
alleviating
bone
model,
thereby
laying
groundwork
translational
research
eriocauli
