Globally distributed mining-impacted environments are underexplored hotspots of multidrug resistance genes

The ISME Journal, Год журнала: 2022, Номер 16(9), С. 2099 - 2113

Опубликована: Июнь 10, 2022

Abstract Mining is among the human activities with widest environmental impacts, and mining-impacted environments are characterized by high levels of metals that can co-select for antibiotic resistance genes (ARGs) in microorganisms. However, ARGs still poorly understood. Here, we conducted a comprehensive study such worldwide, taking advantage 272 metagenomes generated from global-scale data collection two national sampling efforts China. The average total abundance globally distributed studied mine sites was 1572 times per gigabase, being rivaling urban sewage but much higher than freshwater sediments. Multidrug accounted 40% ARG abundance, tended to co-occur multimetal genes, were highly mobile (e.g. on 16% occurring plasmids). Among 1848 high-quality metagenome-assembled genomes (MAGs), 85% carried at least one multidrug gene plus gene. These ARG-carrying MAGs considerably expanded phylogenetic diversity hosts, providing first representatives Archaea domain three bacterial phyla. Moreover, 54 identified as potential pathogens. Our findings suggest worldwide underexplored hotspots genes.

Язык: Английский

---

Deterministic Effect of pH on Shaping Soil Resistome Revealed by Metagenomic Analysis

Environmental Science & Technology, Год журнала: 2023, Номер 57(2), С. 985 - 996

Опубликована: Янв. 5, 2023

Soil is recognized as the major reservoir of antibiotic resistance genes (ARGs), harboring most diverse naturally evolved ARGs on planet. Multidrug are a class ARGs, and their high prevalence in natural soil ecosystems has recently raised concerns. Since these express proton motive force (PMF) driven efflux pumps, studying whether pH determinant for selection multidrug pump thus shaping resistome great interest. In this study, we collected 108 soils with values ranging from 4.37 to 9.69 multiple profiled composition metagenomes metagenome-assembled genomes. We observed enriched acidic resistome, abundances have significant dependence. This reflects benefits activity genes, especially PMF-driven inner membrane transferase. addition, preliminary indicate putative microbial participants by applying ecological analyzing tools such stepwise regression random forest model fitting. The decisive influence more impactful than any other examined factors, consequence, revisited edaphic factors resistome; i.e., deterministic mechanisms could lead bottom-up ARG composition. Such developing herein suggested be considered assessing human-driven transmissions.

Язык: Английский

---

Joining Forces against Antibiotic Resistance: The One Health Solution

Pathogens, Год журнала: 2023, Номер 12(9), С. 1074 - 1074

Опубликована: Авг. 23, 2023

Antibiotic resistance is a significant global health concern that affects both human and animal populations. The One Health approach acknowledges the interconnectedness of health, environment. It emphasizes importance collaboration coordination across these sectors to tackle complex challenges such as antibiotic resistance. In context Health, refers ability bacteria withstand efficacy antibiotics, rendering them less effective or completely ineffective in treating infections. emergence spread antibiotic-resistant pose threat well effectiveness medical treatments veterinary interventions. particular, recognizes use medicine, agriculture, environment are interconnected factors contributing development For example, misuse overuse antibiotics healthcare, including inappropriate prescribing patient non-compliance, can contribute selection resistant bacteria. Similarly, livestock production for growth promotion disease prevention animals subsequent transmission humans through food chain. Addressing requires collaborative involves multiple participants, healthcare professionals, veterinarians, researchers, policymakers.

Язык: Английский

---

Organic fertilization co-selects genetically linked antibiotic and metal(loid) resistance genes in global soil microbiome

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июнь 17, 2024

Abstract Antibiotic resistance genes (ARGs) and metal(loid) (MRGs) coexist in organic fertilized agroecosystems based on their correlations abundance, yet evidence for the genetic linkage of ARG-MRGs co-selected by fertilization remains elusive. Here, an analysis 511 global agricultural soil metagenomes reveals that correlates with a threefold increase number diverse types ARG-MRG-carrying contigs (AMCCs) microbiome (63 types) compared to non-organic soils (22 types). Metatranscriptomic data indicates increased expression AMCCs under higher arsenic stress, co-regulation ARG-MRG pairs. Organic heightens coexistence genomic elements through impacting properties ARG MRG abundances. Accordingly, comprehensive map was constructed delineate distribution coexistent virulence factors mobile metagenome-assembled genomes from lands. The unveils heightened relative abundance potential pathogenicity risks (range 4-6) spread Central North America, Eastern Europe, Western Asia, Northeast China other regions, which acquire risk range 1-3. Our findings highlight co-selects genetically linked ARGs MRGs microbiome, underscore need mitigate these co-resistant safeguard public health.

Язык: Английский

---

A database on the abundance of environmental antibiotic resistance genes

Scientific Data, Год журнала: 2024, Номер 11(1)

Опубликована: Фев. 27, 2024

Abstract Antimicrobial resistance (AMR) poses a severe threat to global health. The wide distribution of environmental antibiotic genes (ARGs), which can be transferred between microbiota, especially clinical pathogens and human commensals, contributed significantly AMR. However, few databases on the spatiotemporal distribution, abundance, health risk ARGs from multiple environments have been developed, absolute level. In this study, we compiled ARG occurrence data generated by high-throughput quantitative PCR platform 1,403 samples in 653 sampling sites across 18 provinces China. database possessed 291,870 records five types habitats abundance 290 ARGs, as well 8,057 30 mobile genetic elements (MGEs) 2013 2020. These conferred major common antibiotics (a total 15 types) represented mechanisms, four ranks. provide information for studies dynamics is useful assessment

Язык: Английский

---

Strategy for Mitigating Antibiotic Resistance by Biochar and Hyperaccumulators in Cadmium and Oxytetracycline Co-contaminated Soil

Environmental Science & Technology, Год журнала: 2021, Номер 55(24), С. 16369 - 16378

Опубликована: Окт. 25, 2021

The global prevalence of antibiotic resistance genes (ARGs) is increasing concern as a serious threat to ecological security and human health. Irrigation with sewage farmland application manure or biosolids in agricultural practices introduce substantial selective agents such antibiotics toxic metals, aggravating the transfer ARGs from soil environment humans via food chain. To address this issue, hyperaccumulator (Sedum plumbizincicola) combined biochar amendment was first used investigate mitigation cadmium oxytetracycline co-contaminated by conducting pot experiment. addition affected distribution plants differently enhancing their but restraining transmission S. plumbizincicola. planting plumbizincicola resulted an increase environment. A structural equation model illustrated that mobile genetic elements played dominant role shaping profile ARGs. Taken together, these findings provide practical understanding for mitigating system complex contamination can have profound significance management regard ARG dissemination control.

Язык: Английский

---

Antibiotic resistance genes in food production systems support One Health opinions

Current Opinion in Environmental Science & Health, Год журнала: 2023, Номер 34, С. 100492 - 100492

Опубликована: Июль 6, 2023

Язык: Английский

---

Perspectives on systematic generation of antibiotic resistance with special emphasis on modern antibiotics

Total Environment Research Themes, Год журнала: 2023, Номер 8, С. 100068 - 100068

Опубликована: Июль 13, 2023

Antibiotics, considered a "wonder medication" in the 20th century, have been exploited to such an extent that bacteria developed resistance against them giving rise highly resistant superbugs, which are able survive presence of medicine is designed stop from growing. The study elucidates classification and molecular mechanisms methods employed by different generations antibiotics with their classes, examples strains. overuse, misuse underuse several antibiotic classes improper disposal/treatment various industries including agriculture, poultry, veterinary healthcare has led generation many species at higher rate. causes, drivers, consequences, environmental impact also addressed. This major global concern amid recent infectious diseases caused bacterial pathogens calls for effective, newer better alternative treatments. If we fail combat resistance, death tolls due small infections would further lead social, economic, medical losses. paper highlights pressing need curb overuse prevent advocates regulation, awareness programs about AMR barriers discovery.

Язык: Английский

---

Gut microbiota research nexus: One Health relationship between human, animal, and environmental resistomes

mLife, Год журнала: 2023, Номер 2(4), С. 350 - 364

Опубликована: Дек. 1, 2023

The emergence and rapid spread of antimicrobial resistance is global public health concern. gut microbiota harboring diverse commensal opportunistic bacteria that can acquire via horizontal vertical gene transfers considered an important reservoir sink antibiotic genes (ARGs). In this review, we describe the reservoirs ARGs their dynamics in both animals humans, use One Health perspective to track transmission ARG-containing between animals, environment, assess impact on human socioeconomic development. resistome evolve environment subject various selective pressures, including administration environmental lifestyle factors (e.g., diet, age, gender, living conditions), interventions through probiotics. Strategies reduce abundance clinically relevant antibiotic-resistant determinants niches are needed ensure mitigation acquired resistance. With help effective measures taken at national, local, personal, intestinal management, it will also result preventing or minimizing infectious diseases. This review aims improve our understanding correlations provide a basis for development management strategies mitigate crisis.

Язык: Английский

---

Effect of polyethylene microplastics on antibiotic resistance genes: A comparison based on different soil types and plant types

Journal of Hazardous Materials, Год журнала: 2024, Номер 472, С. 134581 - 134581

Опубликована: Май 11, 2024

Язык: Английский

---