Growth of microbes in competitive lifestyles promotes increased ARGs in soil microbiota: insights based on genetic traits

Microbiome, Год журнала: 2025, Номер 13(1)

Опубликована: Янв. 13, 2025

The widespread selective pressure of antibiotics in the environment has led to propagation antibiotic resistance genes (ARGs). However, mechanisms by which microbes balance population growth with enrichment ARGs remain poorly understood. To address this, we employed microcosm cultivation at different (i.e., Oxytetracycline, OTC) stresses across concentrations from environmental clinical. Paired shot-gun metagenomics analysis and quantification bacterial growth, trait-based assessment soil microbiota was applied reveal association between key ARG subtypes, representative taxa, functional-gene features that drive ARGs. Our results illuminate resistome variation is closely associated growth. A non-monotonic change abundance richness observed over a concentration gradient none 10 mg/l. Soil exposed intermediate OTC 0.1 0.5 mg/l) showed greater increases total Community compositionally, i.e., Pseudomonadaceae considered boost increase It chromosomally carried kinds multidrug such as mexAB-oprM mexCD-oprJ could mediate intrinsic OTC. Streptomycetaceae shown better adaptive ability than other clinical concentrations. it contributed less represents stress-tolerant lifestyle grows slowly carries fewer In terms community genetic features, aggregated traits further indicates enhancement resource acquisition yield driving abundance. Moreover, optimizations energy production conversion, alongside streamlining bypass metabolic pathways, sub-inhibitory conditions. this study suggest competitive lifestyles are selected under stress nutrient scarcity. They possess substrate utilization capacity carry more ARGs, due they were faster growing leading This expanded application assessments understanding ecology propagation. And finding illustrated changes accompanied switching microbiome, theoretically supports control approach based on principle species exclusion.

Язык: Английский

---

Gut microbiota research nexus: One Health relationship between human, animal, and environmental resistomes

mLife, Год журнала: 2023, Номер 2(4), С. 350 - 364

Опубликована: Дек. 1, 2023

The emergence and rapid spread of antimicrobial resistance is global public health concern. gut microbiota harboring diverse commensal opportunistic bacteria that can acquire via horizontal vertical gene transfers considered an important reservoir sink antibiotic genes (ARGs). In this review, we describe the reservoirs ARGs their dynamics in both animals humans, use One Health perspective to track transmission ARG-containing between animals, environment, assess impact on human socioeconomic development. resistome evolve environment subject various selective pressures, including administration environmental lifestyle factors (e.g., diet, age, gender, living conditions), interventions through probiotics. Strategies reduce abundance clinically relevant antibiotic-resistant determinants niches are needed ensure mitigation acquired resistance. With help effective measures taken at national, local, personal, intestinal management, it will also result preventing or minimizing infectious diseases. This review aims improve our understanding correlations provide a basis for development management strategies mitigate crisis.

Язык: Английский

---

Antimicrobial Residues in Poultry Litter: Assessing the Association of Antimicrobial Persistence with Resistant Escherichia coli Strains

Antibiotics, Год журнала: 2025, Номер 14(1), С. 89 - 89

Опубликована: Янв. 14, 2025

Objective: We set out to evaluate the persistence of sulfachloropyridazine, oxytetracycline, and enrofloxacin in broiler chicken litter following therapeutic oral treatment its association with isolation Escherichia coli resistant these antimicrobials. Methods: Forty chickens were raised under controlled conditions divided into three experimental groups, each treated a different antimicrobial, addition an untreated/control group. Litter samples collected post treatment, analyzed by UPLC-MS/MS, processed for E. coli. The antimicrobial susceptibility was assessed using Kirby–Bauer disk diffusion method. Results: Chemical analysis detected concentrations antimicrobials throughout reaching maxima 42,910.14 μg kg−1, 92,712 9567 kg−1 oxytetracycline plus 4-epi-oxytetracycline, ciprofloxacin, respectively. It estimated that would persist 61, 244, 514 days, A very strong observed between presence residues resistance (p-value < 0.0001, Cramer’s coefficient 0.47), independence level residue concentration 0.5262). Conclusions: contributes selection bacteria, regardless persistent concentrations. These findings highlight need stricter regulations on poultry management, including thresholds monitoring, minimize environmental public health risks. Proper is essential ensure sustainable safe re-use agricultural systems.

Язык: Английский

---

Application of machine learning for antibiotic resistance in water and wastewater: A systematic review

Chemosphere, Год журнала: 2024, Номер 358, С. 142223 - 142223

Опубликована: Май 2, 2024

Язык: Английский

---

Vertical migration and dissipation of oxytetracycline induces the recoverable shift in microbial community and antibiotic resistance

The Science of The Total Environment, Год журнала: 2023, Номер 905, С. 167162 - 167162

Опубликована: Сен. 18, 2023

Язык: Английский

---

Staphylococcus aureus Isolated From Traditional Artisanal Raw Milk Cheese from Southern Brazil: Diversity, Virulence, and Antimicrobial Resistance Profile

Journal of Food Protection, Год журнала: 2024, Номер 87(6), С. 100285 - 100285

Опубликована: Апрель 30, 2024

Staphylococcus aureus is one of the primary pathogenic agents found in cheeses produced with raw milk. Some strains S. are enterotoxigenic, possessing ability to produce toxins responsible for staphylococcal food poisoning when present contaminated foods. This study aimed genotypically characterize, assess antimicrobial resistance profile, and examine enterotoxigenic potential isolated from artisanal colonial cheese. Additionally, a bacterial diversity assessment was conducted by sequencing 16S rRNA gene. The metataxomic profile revealed presence 68 distinct species cheese samples. Fifty-seven isolates were identified, highlighted penicillin 33% isolates, followed clindamycin (28%), erythromycin (26%), tetracycline (23%). evaluated also exhibited inducible clindamycin, nine considered multidrug-resistant (MDR). agr type I most prevalent (62%) among II (24%). ten spa types identified. Although no enterotoxins their associated genes detected samples respectively, Panton-Valentine leukocidin gene (lukS-lukF) 39% isolates. MDR pathogens milk production chain underscores need quality management prevent contamination dissemination strains.

Язык: Английский

---

Characterization of ES10 lytic bacteriophage isolated from hospital waste against multidrug-resistant uropathogenic E. coli

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Март 8, 2024

Escherichia coli is the major causative agent of urinary tract infections worldwide and emergence multi-drug resistant determinants among clinical isolates necessitates development novel therapeutic agents. Lytic bacteriophages efficiently kill specific bacteria seems promising approach in controlling caused by pathogens. This study aimed isolation detailed characterization lytic bacteriophage designated as ES10 capable lysing multidrug-resistant uropathogenic E. . had icosahedral head non-contractile tail genome size was 48,315 base pairs long encoding 74 proteins. Antibiotics resistance, virulence lysogenic cycle associated genes were not found phage genome. Morphological whole analysis showed that member Drexlerviridae Latent time 30 min, burst 90, optimal multiplicity infection 1. stable human blood subsequently 99.34% reduction host bacteria. Calcium chloride shortened adsorption latency period significantly inhibited biofilm formation 99.84% from contaminated fomites. possesses potential to be utilized standard therapy.

Язык: Английский

---

Sources, interactions, influencing factors and ecological risks of microplastics and antibiotic resistance genes in soil: A review

The Science of The Total Environment, Год журнала: 2024, Номер 949, С. 175226 - 175226

Опубликована: Авг. 3, 2024

Язык: Английский

---

A Health Threat from Farm to Fork: Shiga Toxin-Producing Escherichia coli Co-Harboring blaNDM-1 and mcr-1 in Various Sources of the Food Supply Chain

Pathogens, Год журнала: 2024, Номер 13(8), С. 659 - 659

Опубликована: Авг. 6, 2024

The dissemination of resistant pathogens through food supply chains poses a significant public health risk, spanning from farm to fork. This study analyzed the distribution Shiga toxin-producing Escherichia coli (STEC) across various sources within animal-based chain. A total 500 samples were collected livestock, poultry, environment, fisheries, and dairy. Standard microbiological procedures employed isolate identify E. isolates, which further confirmed using MALDI-TOF virulence-associated genes (VAGs) such as stx1, stx2, ompT, hylF, iutA, fimH, iss. phenotypic resistance patterns isolates determined disc diffusion method, followed by molecular identification antibiotic (ARGs) PCR. STEC subjected PCR-based O typing specific primers for different types. Overall, 154 (30.5%) coli, 77 (50%) multidrug-resistant (MDR) coli. Among these, 52 (67.53%) exhibited an array VAGs, 21 (40.38%) based on presence stx1 stx2. Additionally, 12 out (23.07%) identified non-O157 co-harbouring mcr-1 blaNDM-1. O26 was found be most prevalent among results suggest that detection in may lead serious consequences, particularly developing countries with limited healthcare resources.

Язык: Английский

---

Biomass Resource Management and Increasing the Yield Of Biogas Production in the Farm: A Review

Journal of Advances in Environmental Health Research, Год журнала: 2025, Номер 13(2), С. 68 - 89

Опубликована: Апрель 20, 2025

Anaerobic digestion (AD) converts animal and agricultural waste into biogas, offering significant benefits for management renewable energy production. It enhances security, reduces dependence on imports, mitigates greenhouse gas emissions. Despite its potential, AD faces challenges that require improved policies, investments, training. Technological advancements, such as nanotechnology, can further increase biogas production, while byproducts like biofertilizer contribute to farm profitability. However, pathogens in (AW), Escherichia coli Salmonella, pose public health risks through contamination of water, food, surfaces. Efficient livestock is essential reduce environmental impacts, the proper pricing natural resources, including land, landfills, crucial sustainability. The include generation, reduction, pathogen elimination, conversion organic high-quality fertilizer, which supports productivity. remain, smallscale technology, impurities, temperature sensitivity, limited applicability urban areas. To improve economic feasibility, fermentation process be conducted controlled environments using tanks. This study explores strategies effective biomass resource management, focusing optimizing production by-product utilization sustainable development.

Язык: Английский

---