bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Окт. 7, 2022
ABSTRACT
Similar
to
the
human
brain,
Drosophila
glia
may
well
be
divided
into
several
subtypes
that
each
carry
out
specific
functions.
Glial
GPCRs
plays
key
roles
in
crosstalk
between
neurons
and
glia.
Lgr4
(dLgr4
)
is
a
relaxin
receptor
homolog
involved
angiogenesis,
cardiovascular
regulation,
collagen
remodeling,
wound
healing.
Recent
study
suggests
ilp7
might
ligand
for
regulates
escape
behavior
of
larvae.
Here
we
demonstrate
expression
glial
cells,
not
neurons,
necessary
early
development,
adult
behavior,
lifespan.
Reducing
level
but
disrupts
although
knocking
down
other
LGR
family
members
has
no
impact.
Adult-specific
knockdown
reduce
locomotion,
male
reproductive
success,
animal
longevity.
The
investigation
how
contributes
this
behavioral
alteration
will
increase
our
understanding
insulin
signaling
via
selectively
modulates
neuronal
activity
behavior.
PLoS ONE,
Год журнала:
2025,
Номер
20(5), С. e0318406 - e0318406
Опубликована: Май 30, 2025
Evolutionarily
conserved
transforming
growth
factor
β
(TGF-β)
signaling
is
used
in
both
vertebrates
and
invertebrates
to
regulate
a
variety
of
developmental
cellular
processes.
The
baboon
(
babo
)
gene
encoding
Drosophila
type-I
TGF-β
receptor
produces
three
isoforms
via
alternative
splicing:
BaboA,
BaboB,
BaboC.
In
this
study,
we
generated
fly
lines,
each
carrying
an
isoform-specific
GFP
tag,
another
line
with
conjugated
at
the
C-terminus
common
all
isoforms.
Using
these
assessed
(1)
whether
tagged
proteins
function
properly
rescue
assays
(2)
how
isoform
expression
regulated
various
tissues
including
central
nervous
system
(CNS).
A
Gal4
knock-in
locus
was
also
characterized
for
reporter
expression,
mutant
phenotypes,
knockdown
phenotypes.
We
found
that
C-terminal
tag
does
not
interrupt
subcellular
targeting
functions
isoforms,
but
internal
tags
do
so
cell-
fashion.
Nevertheless,
our
results
demonstrated
faithfully
reflect
endogenous
individual
Certain
cell
types
express
single
or
multiple
different
levels,
suggesting
splicing
could
determine
their
levels
depending
on
(or
tissue)
type.
larval
CNS
displays
distinct
patterns
two
BaboA
BaboC
mostly
expressed
neural
cells
originating
during
embryogenesis,
while
broadly
produced
from
embryonic
postembryonic
stages.
Assays
mutants
cell-specific
revealed
broad
roles
played
by
neurogenesis
differentiation
precursor
neurons,
remodeling
processes
persisting
metamorphic
reorganization,
which
are
essential
establishing
adult
CNS.
Taken
together,
study
demonstrates
GFP-tagged
lines
permit
visualization
further
provides
clues
about
stage-specific
isoform.
Frontiers in Physiology,
Год журнала:
2023,
Номер
14
Опубликована: Янв. 19, 2023
Understanding
the
physiological
mechanisms
that
modulate
memory
acquisition
and
consolidation
remains
among
most
ambitious
questions
in
neuroscience.
Massive
efforts
have
been
dedicated
to
deciphering
how
experience
affects
behavior,
different
sensory
phenomena
memory.
Our
ability
encode,
consolidate
retrieve
memories
depends
on
internal
drives,
sleep
stands
out
processes
affect
memory:
one
of
relatable
benefits
is
aiding
occurs
order
both
prepare
brain
learn
new
information,
after
a
learning
task,
those
memories.
Drosophila
lends
itself
study
interactions
between
sleep.
The
fruit
fly
provides
incomparable
genetic
resources,
mapped
connectome,
an
existing
framework
knowledge
molecular,
cellular,
circuit
sleep,
making
remarkable
model
decipher
sophisticated
regulation
by
quantity
quality
Research
has
stablished
not
only
facilitates
wild-type
memory-impaired
animals,
but
deprivation
interferes
with
In
addition,
it
well-accepted
paramount
processes.
Finally,
studies
shown
can
promote
drive.
Nevertheless,
molecular
network
underlying
this
intertwined
relationship
are
still
evasive.
Recent
work
shed
light
neural
substrates
mediate
sleep-dependent
consolidation.
similar
way,
mechanistic
insights
switch
control
sleep-independent
strategies
were
recently
described.
This
review
will
discuss
,
focusing
recent
advances
field
pointing
awaiting
be
investigated.
Journal of Neuroscience Research,
Год журнала:
2023,
Номер
102(1)
Опубликована: Ноя. 28, 2023
Abstract
Similar
to
the
human
brain,
Drosophila
glia
may
well
be
divided
into
several
subtypes
that
each
carries
out
specific
functions.
Glial
GPCRs
play
key
roles
in
crosstalk
between
neurons
and
glia.
Lgr4
(dLgr4)
is
a
relaxin
receptor
homolog
involved
angiogenesis,
cardiovascular
regulation,
collagen
remodeling,
wound
healing.
A
recent
study
suggests
ilp7
might
ligand
for
regulates
escape
behavior
of
larvae.
Here
we
demonstrate
expression
glial
cells,
not
neurons,
necessary
early
development,
adult
behavior,
lifespan.
Reducing
level
cells
disrupts
while
knocking
down
other
LGR
family
members
has
no
impact.
Adult‐specific
knockdown
but
reduce
locomotion,
male
reproductive
success,
animal
longevity.
The
investigation
how
contributes
this
behavioral
alteration
will
increase
our
understanding
insulin
signaling
via
selectively
modulates
neuronal
activity
behavior.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 8, 2024
Summary
An
animal’s
metabolic
state
strongly
influences
its
behavior.
Hungry
animals
prioritize
food
seeking
and
feeding
behaviors,
while
sated
suppress
these
behaviors
to
engage
in
other
activities.
Additionally,
neuronal
activity
synaptic
transmission
are
among
the
most
energy
expensive
processes.
Yet
neurons
do
not
uptake
nutrients
from
circulation.
Instead,
glia
fulfill
this
highly
evolutionary
conserved
function.
Recent
studies
have
shown
that
can
modulate
However,
how
different
subtypes
sense
behavior
is
incompletely
understood.
Here,
we
unravel
two
types
of
glia-mediated
modulation
state-dependent
In
food-deprived
flies,
astrocyte-like
perineurial
promote
foraging
feeding,
respectively,
cortex
behaviors.
We
further
show
adenosine
receptor
intracellular
calcium
levels
subtypes,
which
ultimately
controls
This
study
reveals
a
new
mechanism
animal
accordingly.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 21, 2024
ABSTRACT
This
study
reveals
a
sexually
dimorphic
sNPF-sNPF-R
circuit
in
the
Drosophila
brain
that
regulates
energy
balance
behavior,
specifically
shorter-mating-duration
(SMD)
response
to
sexual
experience.
sNPF
is
predominantly
expressed
neurons,
while
sNPF-R
both
neurons
and
glial
cells,
particularly
astrocyte-like
glia
(ALG)
subset
of
cells
outside
mushroom
body
(MB)
termed
"Rishi
cells"
(RS
cells).
Sexual
experience
induces
global
alterations
calcium
signaling
synaptic
plasticity
within
this
circuit,
with
expressing
RS
playing
critical
role
encoding
experience-related
information
into
behavior
related
mating
duration.
Neuronal
glucose
metabolism,
Tret1l
transporter,
essential
for
maintaining
high
levels
proper
function
near
cells.
novel
intricate
neuron-glia
network
acts
as
circuits
processing
interval
timing
behaviors,
highlighting
dynamic
interplay
between
neural
circuits,
regulating
balance.
Animals
typically
have
either
compound
eyes,
or
camera-type
both
of
which
evolved
repeatedly
in
the
animal
kingdom.
Both
eye
types
include
two
important
kinds
cells:
photoreceptor
cells,
can
be
excited
by
light,
and
non-neuronal
support
cells
(SupCs),
provide
essential
to
photoreceptors.
At
molecular
level
deeply
conserved
genes
that
relate
differentiation
fueled
a
discussion
on
whether
not
shared
evolutionary
origin
might
considered
for
this
cell
type.
In
contrast,
only
handful
studies,
primarily
eyes
Drosophila
melanogaster,
demonstrated
similarities
SupCs.
D.
melanogaster
SupCs
(Semper
primary
pigment
cells)
are
specialized
glia
share
several
with
certain
vertebrate
glia,
including
Müller
glia.
This
led
us
question
if
there
could
signatures
SupCs,
even
functionally
different
such
as
image-forming
larval
camera
sunburst
diving
beetle
Thermonectus
marmoratus.
To
investigate
possibility,
we
used
an
in-depth
comparative
whole-tissue
transcriptomics
approach.
Specifically,
dissected
principal
into
SupC-
retina-containing
regions
generated
respective
transcriptomes.
Our
analysis
revealed
common
features
enrichment
glial
function
(e.g.
gap
junction
proteins
innexin
3),
glycogen
production
(glycogenin),
energy
metabolism
(glutamine
synthetase
1
2).
evaluate
similarities,
compared
our
transcriptomes
those
fly
(Müller
glia)
well
retinas.
T.
marmoratus
were
found
distinct
genetic
overlap
These
results
suggest
form
like
photoreceptors,
may
conserved.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 20, 2023
Animals
generally
have
either
compound
eyes,
which
evolved
repeatedly
in
different
invertebrates,
or
camera
many
times
across
the
animal
kingdom.
Both
eye
types
include
two
important
kinds
of
cells:
photoreceptor
cells,
can
be
excited
by
light,
and
non-neuronal
support
cells
(SupCs),
provide
essential
to
photoreceptors.
Despite
examples
convergence
evolution,
similarities
gross
developmental
plan
molecular
signatures
been
discovered,
even
between
phylogenetically
distant
functionally
types.
For
this
reason,
a
shared
evolutionary
origin
has
considered
for
In
contrast,
only
handful
studies,
primarily
on
eyes
Drosophila
melanogaster
,
demonstrated
SupCs.
D.
SupCs
(Semper
primary
pigment
cells)
are
specialized
glia
that
share
several
with
certain
vertebrate
glia,
including
Müller
glia.
This
led
us
speculate
whether
there
conserved
SupCs,
such
as
image-forming
larval
sunburst
diving
beetle
Thermonectus
marmoratus
.
To
investigate
possibility,
we
used
an
in-depth
comparative
whole-tissue
transcriptomics
approach.
Specifically,
dissected
principal
into
SupC-
retina-containing
regions
generated
respective
transcriptomes.
Our
analysis
revealed
features
enrichment
genes
glial
function
(e.g.
gap
junction
proteins
innexin
3),
glycogen
production
(glycogenin),
energy
metabolism
(glutamine
synthetase
1
2).
evaluate
extent
conservation,
compared
our
transcriptomes
those
fly
(Müller
glia)
well
retinas.
T.
were
found
distinct
genetic
overlap
both
These
results
evidence
deep
conservation
addition
raising
questions
about
eye-specific
animals.
