Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation

Опубликована: Янв. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed molecule neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Язык: Английский

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Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation

Опубликована: Фев. 28, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Язык: Английский

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Neural representation of goal direction in the monarch butterfly brain

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Сен. 20, 2023

Neural processing of a desired moving direction requires the continuous comparison between current heading and goal direction. While neural basis underlying is well-studied, coding remains unclear in insects. Here, we used tetrode recordings tethered flying monarch butterflies to unravel how represented insect brain. recording, maintained robust directions relative virtual sun. By resetting their directions, found neurons whose spatial tuning was tightly linked directions. Importantly, unaffected when changed after compass perturbations, showing that these specifically encode Overall, here discovered invertebrate goal-direction share functional similarities cells reported mammals. Our results give insights into evolutionarily conserved principles goal-directed orientation animals.

Язык: Английский

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Variations on an ancient theme — the central complex across insects

Current Opinion in Behavioral Sciences, Год журнала: 2024, Номер 57, С. 101390 - 101390

Опубликована: Апрель 9, 2024

The central complex (CX) is a highly conserved region of the insect brain, and its ubiquitous occurrence suggests that neural circuits are fundamental importance. While overall layout has not changed since evolution flight, substantial variations exist in internal organization all CX components. By changing details system repeating columns layers, these differences affect almost crystalline thus characteristic neuroarchitecture directly links structure with function. neuropil level changes suggest widespread cellular architecture circuits, data at deeper levels mostly limited to fruit fly Drosophila. Nevertheless, interspecies neuron-level have begun emerge. Whereas small compared astounding degree conservation, they reveal evolvable aspects circuitry, providing promising starting points for future research using comparative circuit-level analysis.

Язык: Английский

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Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 22, 2024

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent

Язык: Английский

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Evolution of neural circuitry and cognition

Biology Letters, Год журнала: 2024, Номер 20(5)

Опубликована: Май 1, 2024

Neural circuits govern the interface between external environment, internal cues and outwardly directed behaviours. To process multiple environmental stimuli integrate these with state requires considerable neural computation. Expansion in network size, most readily represented by whole brain has historically been linked to behavioural complexity, or predominance of cognitive Yet, it is largely unclear which aspects circuit variation impact performance. A key question field evolutionary neurobiology therefore how evolve allow improved performance innovation. We discuss this first exploring volumetric changes areas reflect actual change. explore three major axes evolution-replication, restructuring reconditioning cells circuits-and could relate broader phenotypes variation. This discussion touches on relevant uses limitations volumetrics, while advocating a more circuit-based view cognition. then use framework showcase an example from insect brain, multi-sensory integration processing that shared mushroom bodies central complex. end identifying future trends research area, promise advance neurobiology.

Язык: Английский

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Impact of central complex lesions on innate and learnt visual navigation in ants

Journal of Comparative Physiology A, Год журнала: 2023, Номер 209(4), С. 737 - 746

Опубликована: Фев. 15, 2023

Abstract Wood ants are excellent navigators, using a combination of innate and learnt navigational strategies to travel between their nest feeding sites. Visual navigation in has been studied extensively, however, we have little direct evidence for the underlying neural mechanisms. Here, perform lateralized mechanical lesions central complex (CX) wood ants, midline structure known allow an insect keep track direction sensory cues relative its own orientation control movement. We lesioned two groups observed behaviour arena with large visual landmark present. The first group were naïve when intact such show clear attraction conspicuous landmark. second trained aim food location side general heading towards cue was not altered by lesions, but adjacent position affected. Thus, CX had specific impact on guidance. also that lateralised fine details turning spending less time ipsilateral lesion. results confirm role turn highlight important implementation behaviours rely information from other brain regions.

Язык: Английский

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Cognitive limits of larvalDrosophila: testing for conditioned inhibition, sensory preconditioning, and second-order conditioning

Learning & Memory, Год журнала: 2024, Номер 31(5), С. a053726 - a053726

Опубликована: Май 1, 2024

Drosophila larvae are an established model system for studying the mechanisms of innate and simple forms learned behavior. They have about 10 times fewer neurons than adult flies, it was low total number their that allowed electron microscopic reconstruction brain at synaptic resolution. Regarding mushroom body, a central structure many associative learning in insects, turned out more half classes connection had previously escaped attention. Understanding function these circuit motifs, subsequently confirmed is important current research topic. In this context, we test larval cognitive abilities three tasks characteristically complex those studied. Our data provide evidence (i) conditioned inhibition, as has been reported flies honeybees. Unlike what described honeybees, however, our do not (ii) sensory preconditioning or (iii) second-order conditioning larvae. We discuss methodological features experiments well four specific aspects organization may explain why two observed but .

Язык: Английский

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Conservation and divergence of the retinal homeobox genetic neural lineage between Drosophila melanogaster and Tribolium castaneum

Опубликована: Янв. 1, 2024

Brains are the central organ integrating sensory information and orchestrating behaviour. Their function development of great scientific interest, but their enormous complexity makes them difficult to study. The reduced size insect brains compared mammal brains, together with extraordinary experimental toolkits, make model organisms an excellent study case. Insect have a conserved structure functional subunits, neuropils, at same time, strong divergence across large number species is observed. These aspects offer opportunity developmental processes responsible for evolutionary divergence. complex (CX) very neuropil navigation by orientation motor control. A curious between shift in timing, so-called heterochrony: some CX larva while others develop it during metamorphosis. It has remained enigmatic, how homologous cells divergently different realise these changes. transcription factor (TF) retinal homeobox (rx) expressed anterior brain many animals involved development. In insects, Rx-positive contribute formation CX. this study, I applied concept genetic neural lineage (GNL) brains. GNL includes all neurons expressing certain regulatory gene likely species, particularly GNLs TFs like rx. genetically labelled rx beetle Tribolium castaneum using CRISPR/Cas9-mediated knock-in reporter GFP, as was previously done Drosophila melanogaster. Using two transgenic lines, able compare stages revealing conservation GNL. Cells were analysed terms number, cell body position, projection pattern neurotransmitter content. Based on factors, defined expression domains clusters assessed basically population. showed association CX, most projections entering upper unit noduli. Additionally, there lateral accessory lobe well involvement neuroendocrine system pars intercerebralis. found several cases including additional clusters, organisation arrangements, numbers diverged roles mushroom that do not express neurotransmitters GABA, serotonin or dopamine, nonetheless, data provided framework homologising clusters. Furthermore, volume larval adult melanogaster discovered set serotonergic tangential lower castaneum, which described before. This work reveals interesting divergences brain, now allows studying underpinnings Further, shows potential powerful tool biology. Expanding approach include more could provide insights into evolution

Язык: Английский

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Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation

Опубликована: Янв. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Язык: Английский

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