Identification of Parthenogenesis-Inducing Effector Proteins in Wolbachia

Genome Biology and Evolution, Год журнала: 2024, Номер 16(4)

Опубликована: Март 26, 2024

Abstract Bacteria in the genus Wolbachia have evolved numerous strategies to manipulate arthropod sex, including conversion of would-be male offspring asexually reproducing females. This so-called “parthenogenesis induction” phenotype can be found a number strains that infect arthropods with haplodiploid sex determination systems, parasitoid wasps. Despite discovery microbe-mediated parthenogenesis more than 30 yr ago, underlying genetic mechanisms remained elusive. We used suite genomic, computational, and molecular tools identify characterize two proteins are uniquely parthenogenesis-inducing strong signatures host-associated bacterial effector proteins. These putative structural homology eukaryotic protein domains nucleoporins, key insect determining factor Transformer, eukaryotic-like serine–threonine kinase leucine-rich repeats. Furthermore, these significantly impact cell biology model Saccharomyces cerevisiae. suggest factors our results indicate this would made possible by novel mechanism bacterial-host interaction.

Язык: Английский

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Wolbachia symbionts control sex in a parasitoid wasp using a horizontally acquired gene

Current Biology, Год журнала: 2024, Номер 34(11), С. 2359 - 2372.e9

Опубликована: Апрель 30, 2024

Host reproduction can be manipulated by bacterial symbionts in various ways. Parthenogenesis induction is the most effective type of manipulation for their transmission. Insect sex determined regulation doublesex (dsx) splicing through transformer2 (tra2) and transformer (tra) interaction. Although parthenogenesis has been studied since 1970s, its underlying molecular mechanism unknown. Here we identify a Wolbachia parthenogenesis-induction feminization factor gene (piff) that targets sex-determining genes causes female-producing haplodiploid parasitoid Encarsia formosa. We found elimination repressed expression female-specific dsx enhanced male-specific dsx, which led to production wasp haploid male offspring. Furthermore, E. formosa tra truncated non-functional, functional homolog, termed piff, with an insect origin. PIFF colocalize interact TRA2. Moreover, piff coordinated tra2 Our results demonstrate symbiont acquired manipulate host determination cascade induce wasps. This study reveals insect-to-bacteria horizontal transfer drives evolution animal systems, elucidating striking insect-microbe symbiosis.

Язык: Английский

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The intruders that stole the master key

Nature Reviews Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Язык: Английский

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Alien spiders in a palm house with the first report of parthenogenetic Triaeris stenaspis (Araneae: Oonopidae) infected by Wolbachia from new supergroup X

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Март 19, 2025

Abstract Palm houses in Europe serve as urban biodiversity hot spots for alien spiders. As a result of several years research the Poznań House, we documented occurrence 14 spider species, 9 which were to Europe: Coleosoma floridanum , Hasarius adansoni Howaia mogera Ostearius melanopygius Parasteatoda tabulata tepidariorum Scytodes fusca Spermophora kerinci and Triaeris stenaspis . The most abundant species was C. (39.9%). Three recorded first time Poland: S. We studied endosymbiotic Wolbachia Cardinium parthenogenetic T. this spider. endosymbiont characterized based on sequences six bacterial housekeeping genes: 16S rRNA, coxA fbpA ftsZ gatB hcpA Our phylogenetic reconstruction supergroups revealed that bacteria recovered from formed distinct lineages relation all known supergroups. assigned it novel supergroup X with unique within rRNA genes. discussed faunistic results terms long-term survival rates risk invasion

Язык: Английский

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“Hostbusters”: The Bacterial Endosymbiont Wolbachia of the Parasitoid Wasp Habrobracon hebetor Improves Its Ability to Parasitize Lepidopteran Hosts

Insects, Год журнала: 2025, Номер 16(5), С. 464 - 464

Опубликована: Апрель 28, 2025

Habrobracon hebetor is a globally acknowledged larval ectoparasitoid that widely used to control lepidopteran pests. Wolbachia natural endosymbiont regulates various aspects of the insect host biology. The ability H. paralyze and develop on larvae from five families was tested under laboratory conditions. Two lines wasp were used, "W+" containing naturally occurring supergroup B, "W-", with eradicated by antibiotic treatment, followed propagation 20 subsequent generations. proportions in which paralysis, as well parasitoid oviposition, larval, pupal, adult development observed, usually higher W+ compared W-. In Loxostege sticticalis, differences these indices not statistically significant. Galleria mellonella, Mamestra brassicae, Ostrinia nubilalis, some parasitism significantly than Bombyx mori Plutella xylostella, could complete its life cycle, but levels at initial steps (from paralysis symptoms presence larvae/pupae parasitoid) 2-5 times lower W- (p < 0.01). It can be suggested advantageous for hebetor, it increases success broad range hosts.

Язык: Английский

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Function of doublesex and transformer-2 genes and its respond to environment factors in Dendroctonus armandi Tsai et Li (Coleoptera: Curculionidae: Scolytinae)

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 144508 - 144508

Опубликована: Май 1, 2025

Язык: Английский

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Infection dynamics of endosymbionts that manipulate arthropod reproduction

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Год журнала: 2025, Номер unknown

Опубликована: Май 22, 2025

ABSTRACT A large proportion of arthropod species are infected with endosymbionts, some which selfishly alter host reproduction. The currently known forms parasitic reproductive manipulations male‐killing, feminization, cytoplasmic incompatibility, parthenogenesis induction and distortion sex allocation. While all these phenomena represent adaptations that enhance parasite spread, they differ in the mechanisms involved consequent infection dynamics. We focus here on latter aspect, summarizing existing theoretical literature dynamics manipulation types, completing remaining knowledge gaps where have not been modelled yet. Our unified framework includes minimal model components required to describe effects each manipulation. establish invasion criteria for potential combinations manipulative yielding predictions an endosymbiont's increase from rarity within a population is initially either uninfected or different symbiont strain. consider diplodiploid haplodiploid hosts, as well can between them. reveals endosymbionts priori best prospects necessarily most commonly found ones nature; priority play role too, incompatibility excels this regard. As whole, considerations ease spreads be complemented how easy it achieve particular manipulation, factors influencing probability interspecific switching occurs succeeds.

Язык: Английский

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Wolbachia supergroup A in Enoplognatha latimana (Araneae: Theridiidae) in Poland as an example of possible horizontal transfer of bacteria

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Март 29, 2024

Abstract Wolbachia (phylum Pseudomonadota, class Alfaproteobacteria, order Rickettsiales, family Ehrlichiaceae) is a maternally inherited bacterial symbiont infecting more than half of arthropod species worldwide and constituting an important force in the evolution, biology, ecology invertebrate hosts. Our study contributes to limited knowledge regarding presence intracellular symbiotic bacteria spiders. Specifically, we investigated occurrence infection spider Enoplognatha latimana Hippa Oksala, 1982 (Araneae: Theridiidae) using sample collected north-western Poland. To best our knowledge, this first report E. . A phylogeny based on sequence analysis multiple genes, including 16S rRNA, coxA , fbpA ftsZ gatB gltA groEL hcpA wsp revealed that from represented supergroup was related endosymbionts discovered other hosts, as well insects orders Diptera Hymenoptera. unique for detected gene. The sequences housekeeping genes have been deposited publicly available databases are source molecular data comparative studies. etiology discussed.

Язык: Английский

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Reproductive manipulation: Wolbachia induce host parthenogenesis using a stolen transformer

Current Biology, Год журнала: 2024, Номер 34(11), С. R547 - R549

Опубликована: Июнь 1, 2024

Язык: Английский

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First report on the tropical fire ant, Solonopsis geminate (Fabricius) as flower damaging pest of moringa (Moringa oleifera Lam.) cultivated near the Western Ghats of Dindigul, Tamil Nadu.

Insect Environment, Год журнала: 2023, Номер 26(3)

Опубликована: Сен. 20, 2023

Plant Health Clinic-second anniversarySurely the eye-catching achievement of last quarter is Chandryaan-3.The Vikram lander and Pragyan rover on south pole moon, indeed a proud moment for scientists Indian Space Research Organization (ISRO) rest us too, including life scientists!This an excellent demonstration integration sciences institutions backed by very supportive government enthusiastic Nation.Insect Environment all our space team.In context it was Drosophilidae fly that first sent to USA V-2 rocket 20 February, 1947.Records are there ants carried International Stations.In future Entomologist here can certainly plan experiments with insects in collaboration ISRO.Maui-one Hawaiian Islands hit wildfires mainly beautiful town Lahaina causing immeasurable damage.I have had privilege visiting Maui, three times, its people mostly friendly Polynesians best modernity, whale-watching, crystal clean beaches sea, go make Maui great destination (but costly!!).Add American continent, crazy floods China, blasting temperatures Europe hottest August this year, makes climate changes seem like dreadful monster.Then Moroccan earthquake Libyan flood more shock than change!The again, how does survive or perish these ordeals needs intensive studies.Again, needless emphasise insect expeditions collections need acceleration disaster sensitive areas, before we lose out species!Our plant health clinic, Shree Nidhi Agrochemicals completed two years service farmers.Shreenidhi Agro Chemicals Startup under aegis Rashvee Phytosanitary Services, super state-of-the-art came into being 5th September 2021.The highlights are: It set heart horticulture farms (in Vijayapura, close Bangalore Airport) catering > 20,000 farmers Urban/Rural, Devanahalli, Dodaballapura, Chikaballapura, Shidalghatta, Bagepalli, Gudibande, Gauribidanur, Chintamani, Kolar, Hoskote Karnataka), Chittoor, Madanapalli (Andhra Pradesh), Krishnagiri, Vellore, (Tamil Nadu) etc.Farmers get benefit expert (all postgraduate agriculture-no quacks) discussion, diagnosis dispensation required inputs single window; seed marketing services.

Язык: Английский

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