Gbx2 controls amacrine cell dendrite stratification through Robo1/2 receptors DOI Open Access
Patrick C. Kerstein,

Yessica Santana Agreda,

Bridget M Curran

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 4, 2023

SUMMARY Within the neuronal classes of retina, amacrine cells (ACs) exhibit greatest diversity in morphology and function. We show that selective expression transcription factor Gbx2 is required for cell fate specification dendritic stratification an individual AC subtype mouse retina. identify Robo1 Robo2 as downstream effectors when deleted, phenocopy misprojections seen mutants. Slit1 Slit2, ligands Robo receptors, are localized to OFF layers inner plexiform layer where we observe both Robo1/2 receptors also proper additional subtypes, such Vglut3+ ACs. These results functions a terminal selector single Slit-Robo signaling developmental mechanism ON-OFF pathway segregation

Язык: Английский

Molecular characterization of the sea lamprey retina illuminates the evolutionary origin of retinal cell types DOI Creative Commons
Junqiang Wang, Lin Zhang, Martina Cavallini

и другие.

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Дек. 30, 2024

The lamprey, a primitive jawless vertebrate whose ancestors diverged from all other vertebrates over 500 million years ago, offers unique window into the ancient formation of retina. Using single-cell RNA-sequencing, we characterize retinal cell types in lamprey and compare them to those mouse, chicken, zebrafish. We find six classes 74 distinct types, many shared with species. conservation indicates their emergence early evolution, highlighting primordial designs circuits for rod pathway, ON-OFF discrimination, direction selectivity. diversification amacrine some ganglion appears, however, be lamprey. further infer genetic regulators specifying identify ancestral regulatory elements across species, noting decreased cells. Altogether, our characterization retina illuminates evolutionary origin visual processing

Язык: Английский

Процитировано

5

An ancient apical patterning system sets the position of the forebrain in chordates DOI Creative Commons
Giacomo Gattoni, Daniel Keitley, Ashley Sawle

и другие.

Science Advances, Год журнала: 2025, Номер 11(4)

Опубликована: Янв. 24, 2025

The evolutionary origin of the vertebrate brain remains a major subject debate, as its development from dorsal tubular neuroepithelium is unique to chordates. To shed light on emergence brain, we compared anterior neuroectoderm across deuterostome species, using available single-cell datasets sea urchin, amphioxus, and zebrafish embryos. We identified conserved gene co-expression module, comparable regulatory network (aGRN) controlling apical organ in ambulacrarians, spatially mapped it by multiplexed situ hybridization developing retina hypothalamus Using functional approaches, show Wnt signaling regulating this module like aGRN echinoderms, that overactivation suppresses forebrain identity. This suggests previously undescribed role for amphioxus determining position forebrain. propose Wnt-regulated possible mechanism which set antero-dorsally early chordate evolution.

Язык: Английский

Процитировано

0

Molecular Characterization of the Sea Lamprey Retina Illuminates the Evolutionary Origin of Retinal Cell Types DOI Open Access
Junqiang Wang, Lin Zhang, Martina Cavallini

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Дек. 12, 2023

Abstract The lamprey, a primitive jawless vertebrate whose ancestors diverged from all other vertebrates over 500 million years ago, offers unique window into the primordial formation of retina. Using single-cell RNA-sequencing, we characterized retinal cell types in lamprey and compared their molecular differentiation regulatory networks with those mouse jawed vertebrates. Our analysis revealed six classes 74 distinct types. We discovered multiple conserved shared between lineages, including notably rods cones, ON OFF bipolar cells, starburst amacrine cells. conservation these indicates emergence early evolution, highlighting primal designs circuits for rod pathway, ON-OFF discrimination, direction selectivity. In contrast to this evidence conservation, pathways diversification cells ganglion appear have distinctly two lineages. Furthermore, inferred master regulators specifying both macaque identified common elements across species, underscoring ancestral nature origins governing classes. Altogether, our characterization retina illuminates evolutionary origin visual processing

Язык: Английский

Процитировано

7

Substance P and dopamine form a “push-pull” system that diurnally regulates retinal gain DOI Creative Commons

José Moya‐Díaz,

Patrício Simões, Leon Lagnado

и другие.

Current Biology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

1

Role of PKN1 in Retinal Cell Type Formation DOI Open Access

M. Brunner,

L. Láng,

Louisa Künkel

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(5), С. 2848 - 2848

Опубликована: Фев. 29, 2024

We recently identified PKN1 as a developmentally active gatekeeper of the transcription factor neuronal differentiation-2 (NeuroD2) in several brain areas. Since NeuroD2 plays an important role amacrine cell (AC) and retinal ganglion (RGC) type formation, we aimed to study expression postnatal retina WT Pkn1−/− animals, with particular focus on these two types. show that is broadly expressed gross structure not different between both genotypes. Postnatal levels were elevated upon Pkn1 knockout, retinae showing more NeuroD2+ cells lower portion inner nuclear layer. Accordingly, immunohistochemical analysis revealed increased amount AC adult retinae. There no differences horizontal cell, bipolar glial RGC numbers, nor defective axon guidance optic chiasm or tract knockout. Interestingly, did, however, see specific reduction SMI-32+ α-RGC These results suggest for formation validate future studies focusing specification development.

Язык: Английский

Процитировано

1

Editorial Overview: Molecular neuroscience DOI

Xiang Yu,

Eunjoon Kim

Current Opinion in Neurobiology, Год журнала: 2024, Номер 86, С. 102873 - 102873

Опубликована: Апрель 2, 2024

Процитировано

0

Morphology of Interneurons: Amacrine Cells DOI
Enrica Strettoi

Elsevier eBooks, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0

Retinal ganglion cell circuits and glial interactions in humans and mice DOI
Kang-Chieh Huang, Mohamed M. Tawfik, Melanie A. Samuel

и другие.

Trends in Neurosciences, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

0

Gbx2 controls amacrine cell dendrite stratification through Robo1/2 receptors DOI Open Access
Patrick C. Kerstein,

Yessica Santana Agreda,

Bridget M Curran

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 4, 2023

SUMMARY Within the neuronal classes of retina, amacrine cells (ACs) exhibit greatest diversity in morphology and function. We show that selective expression transcription factor Gbx2 is required for cell fate specification dendritic stratification an individual AC subtype mouse retina. identify Robo1 Robo2 as downstream effectors when deleted, phenocopy misprojections seen mutants. Slit1 Slit2, ligands Robo receptors, are localized to OFF layers inner plexiform layer where we observe both Robo1/2 receptors also proper additional subtypes, such Vglut3+ ACs. These results functions a terminal selector single Slit-Robo signaling developmental mechanism ON-OFF pathway segregation

Язык: Английский

Процитировано

0