Molecular characterization of the sea lamprey retina illuminates the evolutionary origin of retinal cell types
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Дек. 30, 2024
The
lamprey,
a
primitive
jawless
vertebrate
whose
ancestors
diverged
from
all
other
vertebrates
over
500
million
years
ago,
offers
unique
window
into
the
ancient
formation
of
retina.
Using
single-cell
RNA-sequencing,
we
characterize
retinal
cell
types
in
lamprey
and
compare
them
to
those
mouse,
chicken,
zebrafish.
We
find
six
classes
74
distinct
types,
many
shared
with
species.
conservation
indicates
their
emergence
early
evolution,
highlighting
primordial
designs
circuits
for
rod
pathway,
ON-OFF
discrimination,
direction
selectivity.
diversification
amacrine
some
ganglion
appears,
however,
be
lamprey.
further
infer
genetic
regulators
specifying
identify
ancestral
regulatory
elements
across
species,
noting
decreased
cells.
Altogether,
our
characterization
retina
illuminates
evolutionary
origin
visual
processing
Язык: Английский
An ancient apical patterning system sets the position of the forebrain in chordates
Science Advances,
Год журнала:
2025,
Номер
11(4)
Опубликована: Янв. 24, 2025
The
evolutionary
origin
of
the
vertebrate
brain
remains
a
major
subject
debate,
as
its
development
from
dorsal
tubular
neuroepithelium
is
unique
to
chordates.
To
shed
light
on
emergence
brain,
we
compared
anterior
neuroectoderm
across
deuterostome
species,
using
available
single-cell
datasets
sea
urchin,
amphioxus,
and
zebrafish
embryos.
We
identified
conserved
gene
co-expression
module,
comparable
regulatory
network
(aGRN)
controlling
apical
organ
in
ambulacrarians,
spatially
mapped
it
by
multiplexed
situ
hybridization
developing
retina
hypothalamus
Using
functional
approaches,
show
Wnt
signaling
regulating
this
module
like
aGRN
echinoderms,
that
overactivation
suppresses
forebrain
identity.
This
suggests
previously
undescribed
role
for
amphioxus
determining
position
forebrain.
propose
Wnt-regulated
possible
mechanism
which
set
antero-dorsally
early
chordate
evolution.
Язык: Английский
Molecular Characterization of the Sea Lamprey Retina Illuminates the Evolutionary Origin of Retinal Cell Types
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Дек. 12, 2023
Abstract
The
lamprey,
a
primitive
jawless
vertebrate
whose
ancestors
diverged
from
all
other
vertebrates
over
500
million
years
ago,
offers
unique
window
into
the
primordial
formation
of
retina.
Using
single-cell
RNA-sequencing,
we
characterized
retinal
cell
types
in
lamprey
and
compared
their
molecular
differentiation
regulatory
networks
with
those
mouse
jawed
vertebrates.
Our
analysis
revealed
six
classes
74
distinct
types.
We
discovered
multiple
conserved
shared
between
lineages,
including
notably
rods
cones,
ON
OFF
bipolar
cells,
starburst
amacrine
cells.
conservation
these
indicates
emergence
early
evolution,
highlighting
primal
designs
circuits
for
rod
pathway,
ON-OFF
discrimination,
direction
selectivity.
In
contrast
to
this
evidence
conservation,
pathways
diversification
cells
ganglion
appear
have
distinctly
two
lineages.
Furthermore,
inferred
master
regulators
specifying
both
macaque
identified
common
elements
across
species,
underscoring
ancestral
nature
origins
governing
classes.
Altogether,
our
characterization
retina
illuminates
evolutionary
origin
visual
processing
Язык: Английский
Role of PKN1 in Retinal Cell Type Formation
M. Brunner,
L. Láng,
Louisa Künkel
и другие.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(5), С. 2848 - 2848
Опубликована: Фев. 29, 2024
We
recently
identified
PKN1
as
a
developmentally
active
gatekeeper
of
the
transcription
factor
neuronal
differentiation-2
(NeuroD2)
in
several
brain
areas.
Since
NeuroD2
plays
an
important
role
amacrine
cell
(AC)
and
retinal
ganglion
(RGC)
type
formation,
we
aimed
to
study
expression
postnatal
retina
WT
Pkn1−/−
animals,
with
particular
focus
on
these
two
types.
show
that
is
broadly
expressed
gross
structure
not
different
between
both
genotypes.
Postnatal
levels
were
elevated
upon
Pkn1
knockout,
retinae
showing
more
NeuroD2+
cells
lower
portion
inner
nuclear
layer.
Accordingly,
immunohistochemical
analysis
revealed
increased
amount
AC
adult
retinae.
There
no
differences
horizontal
cell,
bipolar
glial
RGC
numbers,
nor
defective
axon
guidance
optic
chiasm
or
tract
knockout.
Interestingly,
did,
however,
see
specific
reduction
SMI-32+
α-RGC
These
results
suggest
for
formation
validate
future
studies
focusing
specification
development.
Язык: Английский
Substance P and dopamine form a “push-pull” system that diurnally regulates retinal gain
Current Biology,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Editorial Overview: Molecular neuroscience
Current Opinion in Neurobiology,
Год журнала:
2024,
Номер
86, С. 102873 - 102873
Опубликована: Апрель 2, 2024
Morphology of Interneurons: Amacrine Cells
Elsevier eBooks,
Год журнала:
2024,
Номер
unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Retinal ganglion cell circuits and glial interactions in humans and mice
Trends in Neurosciences,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Gbx2 controls amacrine cell dendrite stratification through Robo1/2 receptors
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 4, 2023
SUMMARY
Within
the
neuronal
classes
of
retina,
amacrine
cells
(ACs)
exhibit
greatest
diversity
in
morphology
and
function.
We
show
that
selective
expression
transcription
factor
Gbx2
is
required
for
cell
fate
specification
dendritic
stratification
an
individual
AC
subtype
mouse
retina.
identify
Robo1
Robo2
as
downstream
effectors
when
deleted,
phenocopy
misprojections
seen
mutants.
Slit1
Slit2,
ligands
Robo
receptors,
are
localized
to
OFF
layers
inner
plexiform
layer
where
we
observe
both
Robo1/2
receptors
also
proper
additional
subtypes,
such
Vglut3+
ACs.
These
results
functions
a
terminal
selector
single
Slit-Robo
signaling
developmental
mechanism
ON-OFF
pathway
segregation
Язык: Английский