Gbx2 controls amacrine cell dendrite stratification through Robo1/2 receptors DOI Open Access
Patrick C. Kerstein,

Yessica Santana Agreda,

Bridget M Curran

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 4, 2023

SUMMARY Within the neuronal classes of retina, amacrine cells (ACs) exhibit greatest diversity in morphology and function. We show that selective expression transcription factor Gbx2 is required for cell fate specification dendritic stratification an individual AC subtype mouse retina. identify Robo1 Robo2 as downstream effectors when deleted, phenocopy misprojections seen mutants. Slit1 Slit2, ligands Robo receptors, are localized to OFF layers inner plexiform layer where we observe both Robo1/2 receptors also proper additional subtypes, such Vglut3+ ACs. These results functions a terminal selector single Slit-Robo signaling developmental mechanism ON-OFF pathway segregation

Language: Английский

Molecular characterization of the sea lamprey retina illuminates the evolutionary origin of retinal cell types DOI Creative Commons
Junqiang Wang, Lin Zhang, Martina Cavallini

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 30, 2024

The lamprey, a primitive jawless vertebrate whose ancestors diverged from all other vertebrates over 500 million years ago, offers unique window into the ancient formation of retina. Using single-cell RNA-sequencing, we characterize retinal cell types in lamprey and compare them to those mouse, chicken, zebrafish. We find six classes 74 distinct types, many shared with species. conservation indicates their emergence early evolution, highlighting primordial designs circuits for rod pathway, ON-OFF discrimination, direction selectivity. diversification amacrine some ganglion appears, however, be lamprey. further infer genetic regulators specifying identify ancestral regulatory elements across species, noting decreased cells. Altogether, our characterization retina illuminates evolutionary origin visual processing

Language: Английский

Citations

4
An ancient apical patterning system sets the position of the forebrain in chordates DOI Creative Commons
Giacomo Gattoni, Daniel Keitley, Ashley Sawle

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(4)

Published: Jan. 24, 2025

The evolutionary origin of the vertebrate brain remains a major subject debate, as its development from dorsal tubular neuroepithelium is unique to chordates. To shed light on emergence brain, we compared anterior neuroectoderm across deuterostome species, using available single-cell datasets sea urchin, amphioxus, and zebrafish embryos. We identified conserved gene co-expression module, comparable regulatory network (aGRN) controlling apical organ in ambulacrarians, spatially mapped it by multiplexed situ hybridization developing retina hypothalamus Using functional approaches, show Wnt signaling regulating this module like aGRN echinoderms, that overactivation suppresses forebrain identity. This suggests previously undescribed role for amphioxus determining position forebrain. propose Wnt-regulated possible mechanism which set antero-dorsally early chordate evolution.

Language: Английский

Citations

0
Molecular Characterization of the Sea Lamprey Retina Illuminates the Evolutionary Origin of Retinal Cell Types DOI Open Access
Junqiang Wang, Lin Zhang, Martina Cavallini

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 12, 2023

Abstract The lamprey, a primitive jawless vertebrate whose ancestors diverged from all other vertebrates over 500 million years ago, offers unique window into the primordial formation of retina. Using single-cell RNA-sequencing, we characterized retinal cell types in lamprey and compared their molecular differentiation regulatory networks with those mouse jawed vertebrates. Our analysis revealed six classes 74 distinct types. We discovered multiple conserved shared between lineages, including notably rods cones, ON OFF bipolar cells, starburst amacrine cells. conservation these indicates emergence early evolution, highlighting primal designs circuits for rod pathway, ON-OFF discrimination, direction selectivity. In contrast to this evidence conservation, pathways diversification cells ganglion appear have distinctly two lineages. Furthermore, inferred master regulators specifying both macaque identified common elements across species, underscoring ancestral nature origins governing classes. Altogether, our characterization retina illuminates evolutionary origin visual processing

Language: Английский

Citations

7
Substance P and dopamine form a “push-pull” system that diurnally regulates retinal gain DOI Creative Commons

José Moya‐Díaz,

Patrício Simões, Leon Lagnado

et al.

Current Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

1
Role of PKN1 in Retinal Cell Type Formation DOI Open Access

M. Brunner,

L. Láng,

Louisa Künkel

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2848 - 2848

Published: Feb. 29, 2024

We recently identified PKN1 as a developmentally active gatekeeper of the transcription factor neuronal differentiation-2 (NeuroD2) in several brain areas. Since NeuroD2 plays an important role amacrine cell (AC) and retinal ganglion (RGC) type formation, we aimed to study expression postnatal retina WT Pkn1−/− animals, with particular focus on these two types. show that is broadly expressed gross structure not different between both genotypes. Postnatal levels were elevated upon Pkn1 knockout, retinae showing more NeuroD2+ cells lower portion inner nuclear layer. Accordingly, immunohistochemical analysis revealed increased amount AC adult retinae. There no differences horizontal cell, bipolar glial RGC numbers, nor defective axon guidance optic chiasm or tract knockout. Interestingly, did, however, see specific reduction SMI-32+ α-RGC These results suggest for formation validate future studies focusing specification development.

Language: Английский

Citations

1
Editorial Overview: Molecular neuroscience DOI

Xiang Yu,

Eunjoon Kim

Current Opinion in Neurobiology, Journal Year: 2024, Volume and Issue: 86, P. 102873 - 102873

Published: April 2, 2024

Citations

0
Morphology of Interneurons: Amacrine Cells DOI
Enrica Strettoi

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

0
Retinal ganglion cell circuits and glial interactions in humans and mice DOI
Kang-Chieh Huang, Mohamed M. Tawfik, Melanie A. Samuel

et al.

Trends in Neurosciences, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

0
Gbx2 controls amacrine cell dendrite stratification through Robo1/2 receptors DOI Open Access
Patrick C. Kerstein,

Yessica Santana Agreda,

Bridget M Curran

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 4, 2023

SUMMARY Within the neuronal classes of retina, amacrine cells (ACs) exhibit greatest diversity in morphology and function. We show that selective expression transcription factor Gbx2 is required for cell fate specification dendritic stratification an individual AC subtype mouse retina. identify Robo1 Robo2 as downstream effectors when deleted, phenocopy misprojections seen mutants. Slit1 Slit2, ligands Robo receptors, are localized to OFF layers inner plexiform layer where we observe both Robo1/2 receptors also proper additional subtypes, such Vglut3+ ACs. These results functions a terminal selector single Slit-Robo signaling developmental mechanism ON-OFF pathway segregation

Language: Английский

Citations

0