Biodegradation of conventional plastics: Candidate organisms and potential mechanisms

The Science of The Total Environment, Год журнала: 2023, Номер 885, С. 163908 - 163908

Опубликована: Май 5, 2023

Язык: Английский

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An ecological explanation for carbon source-associated denitrification performance in wastewater treatment plants

Water Research, Год журнала: 2023, Номер 247, С. 120762 - 120762

Опубликована: Окт. 21, 2023

Язык: Английский

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Cyanobacteria as cell factories for the photosynthetic production of sucrose

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Фев. 14, 2023

Biofuels and other biologically manufactured sustainable goods are growing in popularity demand. Carbohydrate feedstocks required for industrial fermentation processes have traditionally been supplied by plant biomass, but the large quantities to produce replacement commodity products may prevent long-term feasibility of this approach without alternative strategies sugar feedstocks. Cyanobacteria under consideration as potential candidates production carbohydrate feedstocks, with potentially lower land water requirements relative plants. Several cyanobacterial strains genetically engineered export significant sugars, especially sucrose. Sucrose is not only naturally synthesized accumulated cyanobacteria a compatible solute tolerate high salt environments, also an easily fermentable disaccharide used many heterotrophic bacteria carbon source. In review, we provide comprehensive summary current knowledge endogenous sucrose synthesis degradation pathways. We summarize genetic modifications that found increase secretion. Finally, consider state synthetic microbial consortia rely on sugar-secreting strains, which co-cultivated alongside microbes able directly convert sugars into higher-value compounds (e.g., polyhydroxybutyrates, 3-hydroxypropionic acid, or dyes) single-pot reaction. recent advances reported such cyanobacteria/heterotroph co-cultivation perspective future developments likely realize their bioindustrial potential.

Язык: Английский

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Dynamics of carbon substrate competition among heterotrophic microorganisms

The ISME Journal, Год журнала: 2024, Номер 18(1)

Опубликована: Янв. 1, 2024

Abstract Growing evidence suggests that interactions among heterotrophic microorganisms influence the efficiency and rate of organic matter turnover. These are dynamic shaped by composition availability resources in their surrounding environment. Heterotrophic inhabiting marine environments often encounter fluctuations quality quantity carbon inputs, ranging from simple sugars to large, complex compounds. Here, we experimentally tested how chemical complexity substrates affects competition growth dynamics between two isolates. We tracked cell density using species-specific polymerase chain reaction (PCR) assays measured rates microbial CO2 production along with associated isotopic signatures (13C 14C) quantify impact these on remineralization. The observed densities revealed substrate-driven interactions: one species exhibited a competitive advantage quickly outgrew other when incubated labile compound whereas both seemed coexist harmoniously presence more matter. Rates respiration coincubation isolates enhanced turnover, sometimes nearly 2-fold, compared incubation as mono-cultures. Isotopic respired indicated resulted greater remineralization macromolecular results demonstrate promote high substrate reduces competitiveness promotes partitioning degradative activities into distinct niches, facilitating coordinated utilization pool. Taken together, this study yields new insight plays pivotal role determining within environments.

Язык: Английский

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Deciphering and designing microbial communities by genome-scale metabolic modelling

Computational and Structural Biotechnology Journal, Год журнала: 2024, Номер 23, С. 1990 - 2000

Опубликована: Апрель 22, 2024

Microbial communities are shaped by the complex interactions among organisms and environment. Genome-scale metabolic models (GEMs) can provide deeper insights into complexity ecological properties of various microbial communities, revealing their intricate interactions. Many researchers have modified GEMs for based on specific needs. Thus, need to be comprehensively summarized better understand trends in development. In this review, we key developments deciphering designing using different GEMs. A timeline selected highlights indicated that area is evolving from single-strain level community level. Then, outlined a framework constructing communities. We also resources static dynamic community-level focused role external environmental intracellular shaping assembly Finally, discussed challenges future directions GEMs, focusing integration with quorum sensing mechanisms, ecology interactions, machine learning algorithms, automatic modeling, all which contribute consortia-based applications fields.

Язык: Английский

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Optimizing Bioaugmentation for Pharmaceutical Stabilization of Sewage Sludge: A Study on Short-Term Composting Under Real Conditions

Journal of Fungi, Год журнала: 2025, Номер 11(1), С. 67 - 67

Опубликована: Янв. 16, 2025

A significant concentration of pharmaceuticals has been detected within composted sewage sludge. Their uncomplete removal and lack monitoring during composting neglects their potentially toxic effects when used as a soil organic amendment. Previously, we successfully implemented bioaugmentation–composting system focused on toxicity pharmaceuticals’ reduction. This method, however, comprised long inoculant-acclimatization period, making it an unprofitable technology. Hence, this work aimed to explore shorter yet effective process by simultaneously implementing the inoculation native microbial consortium fungus Penicillium oxalicum XD 3.1 in piles sludge olive prunings. All were subjected frequent inoculation, windrow turning, physicochemical biological parameters. Additionally, both bioaugmentation stability degradation evaluated through different analysis rates calculations. One hundred days earlier than previous attempts, treatments achieved adequate conditions, maintained core populations while improving degrading diversity, around 70–72% pharmaceutical remotion. Nevertheless, only produced favorable results ideal for amendments (acute microtoxicity phytotoxicity). Thus, but equally stable with P. was here.

Язык: Английский

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A new flavor of synthetic yeast communities sees the light

mBio, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

ABSTRACT No organism is an island: organisms of varying taxonomic complexity, including genetic variants a single species, can coexist in particular niches, cooperating for survival while simultaneously competing environmental resources. In recent years, synthetic biology strategies have witnessed surge efforts focused on creating artificial microbial communities to tackle pressing questions about the complexity natural systems and interactions that underpin them. These engineered ecosystems depend number nature their members, allowing complex cell communication designs recreate create diverse interest. Due its experimental simplicity, budding yeast Saccharomyces cerevisiae has been harnessed establish mixture varied populations with potential explore ecology, metabolic bioprocessing, biosensing, pattern formation. Indeed, enable advanced molecule detection dynamics logic operations. Here, we present concise overview state-of-the-art, highlighting examples exploit optogenetics manipulate, through light stimulation, key phenotypes at community level, unprecedented spatial temporal regulation. Hence, envision bright future where application optogenetic approaches (optoecology) illuminates intricate drives innovations engineering strategies.

Язык: Английский

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Harnessing microbial division of labor for biomanufacturing: a review of laboratory and formal modeling approaches

Critical Reviews in Biotechnology, Год журнала: 2025, Номер unknown, С. 1 - 19

Опубликована: Фев. 19, 2025

Bioprocess industries aim to meet the increasing demand for product complexity by designing enhanced cellular and metabolic capabilities host. Monocultures, standard biomanufacturing workhorses, are often restricted in their capability these demands, solution involves genetic modification of Synthetic microbial communities a promising alternative monocultures because they exhibit division labor, enabling efficient resource utilization pathway modularity. This specialization minimizes burden enhances robustness perturbations, providing competitive advantage. Despite this potential, biotechnological or bioprocessing applications remains limited. The recent emergence new innovative community design tools strategies, particularly those harnessing holds promise change outlook. Understanding interactions governing natural can be used identify complementary partners, informing synthetic design. Therefore, we consider engineering labor as viable accelerate progress field. review presents current understanding how enable information perform tasks otherwise unfeasible individual organisms. We then evaluate laboratory formal modeling approaches specifically developed to: elucidate physiology, guide experimental design, improve our complex assisting By synthesizing insights, present comprehensive framework that advances use applications.

Язык: Английский

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Toward merging bottom–up and top–down model-based designing of synthetic microbial communities

Current Opinion in Microbiology, Год журнала: 2022, Номер 69, С. 102169 - 102169

Опубликована: Июнь 25, 2022

Язык: Английский

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Guided by the principles of microbiome engineering: Accomplishments and perspectives for environmental use

mLife, Год журнала: 2022, Номер 1(4), С. 382 - 398

Опубликована: Ноя. 3, 2022

Although the accomplishments of microbiome engineering highlight its significance for targeted manipulation microbial communities, knowledge and technical gaps still limit applications in biotechnology, especially environmental use. Addressing challenges refractory pollutants fluctuating conditions requires an adequate understanding theoretical achievements practical engineering. Here, we review recent cutting-edge studies on strategies their classical bioremediation. Moreover, a framework is summarized combining both top-down bottom-up approaches toward improved applications. A strategy to engineer microbiomes use, which avoids build-up toxic intermediates that pose risk human health, suggested. We anticipate highlighted will be beneficial address difficult such as degrading multiple sustain performance engineered situ with indigenous microorganisms under conditions.

Язык: Английский

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