Algorithms,
Год журнала:
2023,
Номер
16(12), С. 571 - 571
Опубликована: Дек. 17, 2023
At
present,
synthetic
biology
applications
are
based
on
the
programming
of
bacteria
with
custom-designed
genetic
circuits
through
application
a
top-down
strategy.
These
programs
that
implement
certain
algorithm,
bacterium
being
agent
or
shell
responsible
for
execution
program
in
given
environment.
In
this
work,
we
study
possibility
instead
synthesized
circuit,
it
is
circuit
itself
which
emerges
as
result
evolution
simulated
an
evolutionary
algorithm.
This
conducted
by
performing
silico
experiments
community
composed
one
species
strain
behaves
pathogenic
against
rest
non-pathogenic
also
part
bacterial
consortium.
The
goal
eradication
agents
bacteria.
results
obtained
suggest
plausibility
design
appropriate
resulting
from
bottom-up
strategy
and
therefore
experimental
feasibility
ACS Applied Materials & Interfaces,
Год журнала:
2023,
Номер
15(46), С. 53488 - 53497
Опубликована: Ноя. 6, 2023
Gas
fermentation
offers
a
carbon-neutral
route
for
producing
industrial
feedstocks
using
autotrophic
microbes
to
convert
carbon
dioxide
(CO2)
in
waste
gases,
such
as
emissions
and
biogas,
into
valuable
chemicals
or
biofuels.
However,
slow
microbial
metabolism
owing
low
gaseous
solubility
causes
significant
challenges
gas
fermentation.
Although
chemical
genetic
manipulations
have
been
explored
improve
fermentation,
they
are
either
nonsustainable
complex.
Herein,
an
artificial
soil-like
material
(SLM)
inspired
by
natural
soil
was
fabricated
the
growth
ofCupriavidus
necatorfor
enhanced
poly-β-hydroxybutyrate
(PHB)
biosynthesis
from
CO2
hydrogen
(H2).
Porous
SLM
comprises
low-cost
nanoclay,
boehmite,
starch
serves
biocarrier
facilitate
colonization
of
bacteria
delivery
bacteria.
With
3.0
g/L
addition,
water
increased
∼4
times
biomass
PHB
production
boosted
29
102%,
respectively,
24
h
culture.
In
positive
modulation
observed
biosynthesis.
biosynthesis-associated
gene
expression
found
be
response
addition.
The
concentrations
intermediates
metabolic
pathway
biosynthesis,
pyruvate
acetyl-CoA,
well
reducing
energy
(ATP
NADPH)
significantly
with
also
demonstrated
merits
easy
fabrication,
high
stability,
recyclability,
plasticity,
thereby
indicating
its
considerable
potential
large-scale
application
Microbial Biotechnology,
Год журнала:
2024,
Номер
17(10)
Опубликована: Окт. 1, 2024
The
transcriptional
architecture
of
phages
can
deepen
our
understanding
the
phage-host
infection
process
and
be
key
importance
for
phage
engineering
biotechnological
applications.
Here,
we
applied
ONT-cappable-sequencing,
a
long-read
RNA-sequencing
technique,
to
study
regulatory
mechanisms
Pseudomonas
infecting
giant
201ϕ2-1.
We
identified
67
promoters
132
terminators
that
together
represent
92
units.
A
full
comparison
these
data
transcriptome
model
ϕKZ
confirmed
programs
prototypes
Serwervirus
Phikzvirus
genera
are
largely
conserved,
despite
some
subtle
differences.
Evidence
supporting
shared
include
identification
highly
similar
sequence
motifs
elements
in
both
conservation
loci
relative
homologous
genes
each
phage.
Moreover,
discovered
sRNA
201ϕ2-1
is
conserved
among
prototype
members
different
genera.
Sequencing
host
genome
resulted
its
reclassification
as
atacamensis,
close
important
agricultural
biocontrol
agent
chlororaphis.
Finally,
conducted
vivo
assays
eight
found
them
strongly
terminate
transcription
P.
Control
from
have
rich
history
applications
biotechnology.
In
studies,
demonstrate
new
insight
into
program
potential
novel
useful
tools
synthetic
biology
circuitry.
Algorithms,
Год журнала:
2023,
Номер
16(12), С. 571 - 571
Опубликована: Дек. 17, 2023
At
present,
synthetic
biology
applications
are
based
on
the
programming
of
bacteria
with
custom-designed
genetic
circuits
through
application
a
top-down
strategy.
These
programs
that
implement
certain
algorithm,
bacterium
being
agent
or
shell
responsible
for
execution
program
in
given
environment.
In
this
work,
we
study
possibility
instead
synthesized
circuit,
it
is
circuit
itself
which
emerges
as
result
evolution
simulated
an
evolutionary
algorithm.
This
conducted
by
performing
silico
experiments
community
composed
one
species
strain
behaves
pathogenic
against
rest
non-pathogenic
also
part
bacterial
consortium.
The
goal
eradication
agents
bacteria.
results
obtained
suggest
plausibility
design
appropriate
resulting
from
bottom-up
strategy
and
therefore
experimental
feasibility
