One‐Step Nanoimprinting of Fe2O3/AgBr Thin Films for Dark‐Light Active Antibiofilm and Bacterial‐Free Cell Culture Surfaces DOI

Anindita Thakur,

Shivani Singh, Ramakrishnan Ganesan

и другие.

Small Methods, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

Abstract The resuscitation of bacteria through biofilms presents a critical challenge in controlling microbial pathogenesis and addressing antimicrobial resistance. Continuous antibiofilm activity, particularly on frequently contacted surfaces, is therefore critical. In this study, scalable introduced, one‐step fabrication Fe 2 O 3 /AgBr nanoimprints using polymerizable sol–gel (PSG) approach to create functional nanostructured thin films with strong properties. , visible‐light photocatalyst, coupled AgBr, photosensitizer dark‐active antimicrobial, forming heterojunction that demonstrated potent antibacterial activity against Escherichia coli Pseudomonas putida under both dark light conditions. heterojunctions exhibit significant biofilm inhibition the dark, robust biofilm‐forming P. while visible irradiation ensures complete clearance. These surfaces also achieve optimal reactive oxygen species (ROS) production, selectively targeting without compromising integrity mammalian cells. biocompatibility confirmed MTT, TBARS, apoptosis assays, demonstrating non‐cytotoxic nature substrates. Moreover, enable cell patterning recovery cells from contamination, highlighting their potential creating bacterial‐free environments for culture. This innovative method offers promising route next‐generation, self‐cleaning coatings, combining continuous excellent scalability.

Язык: Английский

Silver Nanocubes/Nano Mica Platelets Flexible Nanohybrid Substrates Modified by Zinc Oxide Quantum Dots with Synergistic 3D Lightning Rod Effect, and Electromagnetic and Chemical Enhancements for Highly Sensitive SERS Bacterial Biosensor DOI
Ming‐Chang Lu, Chih‐Hao Chen,

Yung‐Chi Yang

и другие.

Materials Today Nano, Год журнала: 2025, Номер unknown, С. 100601 - 100601

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

1

Oyster shell-derived CuFe2O4-Hap nanocomposite for healthy houses: Bacterial and formaldehyde elimination DOI
Jinfeng Lü,

Boji Zhou,

Xiaoyan Zhang

и другие.

Chemical Engineering Journal, Год журнала: 2023, Номер 477, С. 147054 - 147054

Опубликована: Ноя. 1, 2023

Язык: Английский

Процитировано

18

Integration of bacteria-imprinted polymer with aluminium transducers: Polymeric electrical behaviour to ionic species DOI
Sing-Mei Tan, Subash C.B. Gopinath, Mohd Hafizuddin Ab Ghani

и другие.

Journal of the Taiwan Institute of Chemical Engineers, Год журнала: 2025, Номер 171, С. 106061 - 106061

Опубликована: Март 2, 2025

Язык: Английский

Процитировано

1

Accurate Prediction of Antimicrobial Susceptibility for Point‐of‐Care Testing of Urine in Less than 90 Minutes via iPRISM Cassettes DOI Creative Commons
Jiang Xin,

T. Borkum,

Sagi Shprits

и другие.

Advanced Science, Год журнала: 2023, Номер 10(31)

Опубликована: Авг. 16, 2023

The extensive and improper use of antibiotics has led to a dramatic increase in the frequency antibiotic resistance among human pathogens, complicating infectious disease treatments. In this work, method for rapid antimicrobial susceptibility testing (AST) is presented using microstructured silicon diffraction gratings integrated into prototype devices, which enhance bacteria-surface interactions promote bacterial colonization. microstructures act also as optical sensors monitoring growth upon exposure real-time label-free manner via intensity-based phase-shift reflectometric interference spectroscopic measurements (iPRISM). Rapid AST clinical isolates Escherichia coli (E. coli) from urine established assay applied directly on unprocessed samples urinary tract infection patients. When coupled with machine learning algorithm trained samples, iPRISM able predict or new sample an Area Under Receiver Operating Characteristic curve (AUC) ∼ 0.85 1 h, AUC > 0.9 90 min, when compared state-of-the-art automated methods used clinic while being order magnitude faster.

Язык: Английский

Процитировано

9

Improving antibacterial ability of Ti-Cu thin films with co-sputtering method DOI Creative Commons
Samaneh Mahmoudi Qashqay, Mohammad‐Reza Zamani‐Meymian,

Seyed Javad Sadati

и другие.

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Окт. 3, 2023

Abstract Due to the resistance of some bacteria antibiotics, research in field dealing with bacterial infections is necessary. A practical approach utilized this study involves preparation an antibacterial thin film on surfaces, which can effectively inhibit and reduce biofilm formation adherence. In study, we report fabrication bactericidal titanium (Ti) copper (Cu) surfaces a powerful co-sputtering method. This method provides situation constituent elements are deposited simultaneously control composition film. Prepared samples were examined by energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), diffraction (XRD), atomic force (AFM), contact angle measurements. To evaluate behavior, used two strains Gram-negative Escherichia coli ( E. ) Gram-positive Staphylococcus aureus S. ). Antibacterial activity prepared sample was assessed determining number colony-forming units per milliliter (CFU/ml) using standard viable cell count assay. Results indicated that as Cu concentration increased, nanoscale became rougher, roughness values rising from 11.85 49.65 nm, increased 40 80 degrees, indicating hydrophilic character. These factors play significant role properties surface. The Ti-Cu films displayed superior ability, 99.9% reduction (equivalent 5-log reduction) viability after 2 h compared Ti alone against both strains. Field emission (FE-SEM) images verified cells physically deformed damaged ultrastructure observed. findings highlight adding improve ability surface while inhibiting Therefore, 14 -Cu 86 highest percentage had best rate. Investigation toxicity Cu-Ti conducted MTT assay, revealed their biocompatibility absence cytotoxicity, further confirming potential promising biomaterials for various applications.

Язык: Английский

Процитировано

9

A Reversible and Dynamic Surface Functionalization for Fluidity Controlled Multivalent Recognition of Lectins and Bacteria DOI Creative Commons

Thomas Hix‐Janssens,

Adam Tillo, H. O. Isaieva

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 26, 2025

Abstract The paper reports the design of multivalent bacterial receptors based on reversible self‐assembled monolayers (rSAMs) gold and glass substrates, mimicking ligand display host cells extracellular matrices. layers consist α‐(4‐amidinophenoxy)alkanes decorated at ω‐position with β‐galactose (Gal) or sialic acid (SA). former acts as a mobile binding to complementary adhesin, LecA, key virulence factor multi‐drug‐resistant bacterium Pseudomonas aeruginosa (PA). Binary amphiphile mixtures containing either these ligands, spontaneously self‐assemble carboxylic terminated SAMs surfaces form rSAMs that are easily tunable respect ratio. It is shown this results in ability construct multi‐reusable featuring strong affinity for adhesin recognitive bacteria, latter demonstrated by incubating culture PA oral commensal species Streptococcus gordonii (SG) Gal SA functionalized rSAMs. In contrast display, covalently attached “static” ligands exhibited low LecA affinity. This approach wet chemical surface functionalization unique imparting both rapid restorability adaptability, compatible heteromultivalent receptor designs boosting lectin bacteria specificity.

Язык: Английский

Процитировано

0

Eco-friendly Multifunctional CeO2/ZnMn2O4 Heterojunction with Robust Photocatalysis for Rapid Bacteria-Killing and Highly Effective Eradication of Formaldehyde Simultaneously DOI
Chih‐Hsuan Huang, Congyang Mao, Shuilin Wu

и другие.

Journal of environmental chemical engineering, Год журнала: 2025, Номер unknown, С. 116945 - 116945

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

Optically accessible, 3D-printed flow chamber with integrated sensors for the monitoring of oral multispecies biofilm growth in vitro DOI Creative Commons

Nicolas Debener,

Nils Heine,

Beate Legutko

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12

Опубликована: Ноя. 11, 2024

The formation of pathogenic multispecies biofilms in the human oral cavity can lead to implant-associated infections, which may ultimately result implant failure. These infections are neither easily detected nor readily treated. Due high complexity biofilms, detailed mechanisms bacterial dysbiotic shift not yet even fully understood. In order study more detail and develop prevention strategies fight

Язык: Английский

Процитировано

2

One‐Step Nanoimprinting of Fe2O3/AgBr Thin Films for Dark‐Light Active Antibiofilm and Bacterial‐Free Cell Culture Surfaces DOI

Anindita Thakur,

Shivani Singh, Ramakrishnan Ganesan

и другие.

Small Methods, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

Abstract The resuscitation of bacteria through biofilms presents a critical challenge in controlling microbial pathogenesis and addressing antimicrobial resistance. Continuous antibiofilm activity, particularly on frequently contacted surfaces, is therefore critical. In this study, scalable introduced, one‐step fabrication Fe 2 O 3 /AgBr nanoimprints using polymerizable sol–gel (PSG) approach to create functional nanostructured thin films with strong properties. , visible‐light photocatalyst, coupled AgBr, photosensitizer dark‐active antimicrobial, forming heterojunction that demonstrated potent antibacterial activity against Escherichia coli Pseudomonas putida under both dark light conditions. heterojunctions exhibit significant biofilm inhibition the dark, robust biofilm‐forming P. while visible irradiation ensures complete clearance. These surfaces also achieve optimal reactive oxygen species (ROS) production, selectively targeting without compromising integrity mammalian cells. biocompatibility confirmed MTT, TBARS, apoptosis assays, demonstrating non‐cytotoxic nature substrates. Moreover, enable cell patterning recovery cells from contamination, highlighting their potential creating bacterial‐free environments for culture. This innovative method offers promising route next‐generation, self‐cleaning coatings, combining continuous excellent scalability.

Язык: Английский

Процитировано

0