Algal Research, Год журнала: 2024, Номер unknown, С. 103888 - 103888
Опубликована: Дек. 1, 2024
Язык: Английский
Plants, Год журнала: 2024, Номер 13(13), С. 1834 - 1834
Опубликована: Июль 3, 2024
Phosphorus (P) is an important and irreplaceable macronutrient. It central to energy information storage exchange in living cells. P element with a "broken geochemical cycle" since it lacks abundant volatile compounds capable of closing the cycle. fertilizers are critical for global food security, but reserves minable scarce non-evenly distributed between countries world. Accordingly, risks crisis due limited access expected be graver than those entailed by competition fossil hydrocarbons. Paradoxically, despite scarcity value reserves, its usage extremely inefficient: current waste rate reaches 80% giving rise plethora unwanted consequences such as eutrophication leading harmful algal blooms. Microalgal biotechnology promising solution tackle this challenge. The proposed review briefly presents relevant aspects microalgal metabolism cell reserve composition turnover, regulation uptake kinetics maximization efficiency focus on novel knowledge. multifaceted role polyPhosphates, largest depot P, discussed emphasis toxicity mediated short-chain polyPhosphates. Opportunities hurdles bioremoval via from streams cultures, either suspended or immobilized, discussed. Possible avenues P-rich biomass biofertilizer production extraction valuable polyPhosphates other bioproducts considered. concludes comprehensive assessment potential ensuring sustainable phosphorus.
Язык: Английский
Процитировано
4
Trends in Food Science & Technology, Год журнала: 2024, Номер 152, С. 104696 - 104696
Опубликована: Авг. 31, 2024
Язык: Английский
Процитировано
3
Yeast, Год журнала: 2024, Номер unknown
Опубликована: Сен. 11, 2024
ABSTRACT Polyphosphate (polyP) is an intriguing molecule that found in almost any organism, covering a multitude of cellular functions. In industry, polyP used due to its unique physiochemical properties, including pH buffering, water binding, and bacteriostatic activities. Despite the importance polyP, analytics still challenging, with gold standard being 31 P NMR. Here, we present simple staining method using fluorescent dye JC‐D7 for semi‐quantitative evaluation yeast extracts. Notably, fluorescence response was affected by concentration polymer chain length 0.5–500 µg/mL range. Hence, samples unknown compositions, cannot be absolute quantification. Fluorescence unaffected inorganic phosphate up 50 mM. Trace elements (FeSO 4 > CuSO CoCl 2 ZnSO ) toxic mineral salts (PbNO 3 HgCl diminished polyP–induced fluorescence, affecting applicability containing polyP–metal complexes. The only marginally other parameters, such as temperature. After validation, this assay elucidate degree production strains carrying gene deletions (poly)phosphate homeostasis. results suggest provides robust sensitive detecting extracts likely microbes. simplicity enables high‐throughput screening microbes fully potentially enhance biotechnological production, ultimately contributing sustainable phosphorus utilization.
Язык: Английский
Процитировано
3
Current Opinion in Biotechnology, Год журнала: 2025, Номер 92, С. 103266 - 103266
Опубликована: Фев. 10, 2025
Язык: Английский
Процитировано
0
Current Opinion in Biotechnology, Год журнала: 2025, Номер 93, С. 103300 - 103300
Опубликована: Март 27, 2025
Язык: Английский
Процитировано
0
Synthetic and Systems Biotechnology, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Авг. 29, 2024
Abstract Inorganic polyphosphate, a linear polymer of orthophosphate residues linked by phosphoanhydride bonds, occurs in all three domains life and plays diverse prominent role metabolism cellular regulation. While the polyphosphate its physiological significance have been well studied bacteria eukaryotes including human, there are only few studies archaea available so far. In Crenarchaeota members Sulfolobaceae , presence degradation via exopolyphosphatase has reported is some evidence for functional metal ion chelation, biofilm formation, adhesion motility, however, nature crenarchaeal kinase still unknown. Here we used model organism Sulfolobus acidocaldarius to study enzymes involved synthesis. The two genes annotated as thymidylate ( saci_2019 saci_2020 ), localized downstream exopolyphosphatase, were identified missing S. Sa PPK3). Thymidylate activity was confirmed Saci_0893. Notably Saci_2020 showed no on own but served regulatory subunit (rPPK3) able enhance catalytically active Saci_2019 (cPPK3). Heteromeric reversible shows clear preference polyP-dependent nucleotide activity, i.e. formation ATP from ADP (12.4 U/mg) lower extent GDP GTP whereas AMP does not serve substrate. PPK direction ATP-dependent polyP synthesis rather low (0.25 U/mg); phosphoryl donor. A combined experimental modelling approach using quantitative 31 P NMR allowed follow enzyme reaction both PolyP observed when ATP/ADP ratio kept high, an recycling system. absence such system, incubations with would reach equilibrium state between 3 4, independent initial conditions. Structural sequence comparisons phylogenetic analysis reveal that member new family, named PPK3, within family P-loop superfamily, clearly separated PPK2. Our show polyP, addition function phosphate storage, special importance energy homeostasis due reversibility serves buffer under charge enabling quick response changes demand.
Язык: Английский
Процитировано
2
Current Opinion in Biotechnology, Год журнала: 2024, Номер 90, С. 103207 - 103207
Опубликована: Сен. 19, 2024
Язык: Английский
Процитировано
2
Current Opinion in Biotechnology, Год журнала: 2024, Номер 90, С. 103198 - 103198
Опубликована: Сен. 14, 2024
Язык: Английский
Процитировано
1
Food Research International, Год журнала: 2024, Номер 197, С. 115177 - 115177
Опубликована: Окт. 5, 2024
Язык: Английский
Процитировано
1