In vivo quantification of polyphosphate in Microcystis aeruginosa using DAPI staining DOI
Rui Zhu, Xiao Tan, Zhipeng Duan

et al.

Algal Research, Journal Year: 2024, Volume and Issue: unknown, P. 103888 - 103888

Published: Dec. 1, 2024

Language: Английский

Getting Grip on Phosphorus: Potential of Microalgae as a Vehicle for Sustainable Usage of This Macronutrient DOI Creative Commons
Alexei Solovchenko, Maxence Plouviez, Inna Khozin‐Goldberg

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(13), P. 1834 - 1834

Published: July 3, 2024

Phosphorus (P) is an important and irreplaceable macronutrient. It central to energy information storage exchange in living cells. P element with a "broken geochemical cycle" since it lacks abundant volatile compounds capable of closing the cycle. fertilizers are critical for global food security, but reserves minable scarce non-evenly distributed between countries world. Accordingly, risks crisis due limited access expected be graver than those entailed by competition fossil hydrocarbons. Paradoxically, despite scarcity value reserves, its usage extremely inefficient: current waste rate reaches 80% giving rise plethora unwanted consequences such as eutrophication leading harmful algal blooms. Microalgal biotechnology promising solution tackle this challenge. The proposed review briefly presents relevant aspects microalgal metabolism cell reserve composition turnover, regulation uptake kinetics maximization efficiency focus on novel knowledge. multifaceted role polyPhosphates, largest depot P, discussed emphasis toxicity mediated short-chain polyPhosphates. Opportunities hurdles bioremoval via from streams cultures, either suspended or immobilized, discussed. Possible avenues P-rich biomass biofertilizer production extraction valuable polyPhosphates other bioproducts considered. concludes comprehensive assessment potential ensuring sustainable phosphorus.

Language: Английский

Citations

4
Wastewater biorefineries: exploring biological phosphorus removal and integrated recovery solutions DOI
Samarpita Roy,

Jette F. Petersen,

Sarah Müller

et al.

Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 92, P. 103266 - 103266

Published: Feb. 10, 2025

Language: Английский

Citations

0
Rapid Fluorescence Assay for Polyphosphate in Yeast Extracts Using JC‐D7 DOI Creative Commons
Alexander Deitert, Jana Fees, Anna Mertens

et al.

Yeast, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 11, 2024

ABSTRACT Polyphosphate (polyP) is an intriguing molecule that found in almost any organism, covering a multitude of cellular functions. In industry, polyP used due to its unique physiochemical properties, including pH buffering, water binding, and bacteriostatic activities. Despite the importance polyP, analytics still challenging, with gold standard being 31 P NMR. Here, we present simple staining method using fluorescent dye JC‐D7 for semi‐quantitative evaluation yeast extracts. Notably, fluorescence response was affected by concentration polymer chain length 0.5–500 µg/mL range. Hence, samples unknown compositions, cannot be absolute quantification. Fluorescence unaffected inorganic phosphate up 50 mM. Trace elements (FeSO 4 > CuSO CoCl 2 ZnSO ) toxic mineral salts (PbNO 3 HgCl diminished polyP–induced fluorescence, affecting applicability containing polyP–metal complexes. The only marginally other parameters, such as temperature. After validation, this assay elucidate degree production strains carrying gene deletions (poly)phosphate homeostasis. results suggest provides robust sensitive detecting extracts likely microbes. simplicity enables high‐throughput screening microbes fully potentially enhance biotechnological production, ultimately contributing sustainable phosphorus utilization.

Language: Английский

Citations

3
Editorial overview: Pioneering innovations in food biotechnology DOI
Christoph Wittmann,

K Yoshida

Current Opinion in Biotechnology, Journal Year: 2025, Volume and Issue: 93, P. 103300 - 103300

Published: March 27, 2025

Language: Английский

Citations

0
A Systematic Study of Regulating Inorganic Polyphosphates Production in Saccharomyces Cerevisiae DOI Creative Commons
Zipeng Chen, Yanling Wu, L. Y. Qin

et al.

Synthetic and Systems Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
The archaeal family 3 polyphosphate kinase reveals a function of polyphosphate as energy buffer under low energy charge DOI Open Access

Svenja Höfmann,

Christian Schmerling,

Christina Stracke

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

Abstract Inorganic polyphosphate, a linear polymer of orthophosphate residues linked by phosphoanhydride bonds, occurs in all three domains life and plays diverse prominent role metabolism cellular regulation. While the polyphosphate its physiological significance have been well studied bacteria eukaryotes including human, there are only few studies archaea available so far. In Crenarchaeota members Sulfolobaceae , presence degradation via exopolyphosphatase has reported is some evidence for functional metal ion chelation, biofilm formation, adhesion motility, however, nature crenarchaeal kinase still unknown. Here we used model organism Sulfolobus acidocaldarius to study enzymes involved synthesis. The two genes annotated as thymidylate ( saci_2019 saci_2020 ), localized downstream exopolyphosphatase, were identified missing S. Sa PPK3). Thymidylate activity was confirmed Saci_0893. Notably Saci_2020 showed no on own but served regulatory subunit (rPPK3) able enhance catalytically active Saci_2019 (cPPK3). Heteromeric reversible shows clear preference polyP-dependent nucleotide activity, i.e. formation ATP from ADP (12.4 U/mg) lower extent GDP GTP whereas AMP does not serve substrate. PPK direction ATP-dependent polyP synthesis rather low (0.25 U/mg); phosphoryl donor. A combined experimental modelling approach using quantitative 31 P NMR allowed follow enzyme reaction both PolyP observed when ATP/ADP ratio kept high, an recycling system. absence such system, incubations with would reach equilibrium state between 3 4, independent initial conditions. Structural sequence comparisons phylogenetic analysis reveal that member new family, named PPK3, within family P-loop superfamily, clearly separated PPK2. Our show polyP, addition function phosphate storage, special importance energy homeostasis due reversibility serves buffer under charge enabling quick response changes demand.

Language: Английский

Citations

2
Polyphosphate in food systems: Their roles and applications in foods and contribution to sustainable processing practices DOI Creative Commons
Wilson Josè Fernandes Lemos,

Davide Santinello,

Somaeyh Mohammadzadeh

et al.

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 152, P. 104696 - 104696

Published: Aug. 31, 2024

Language: Английский

Citations

2
Polyphosphate accumulation in microalgae and cyanobacteria: recent advances and opportunities for phosphorus upcycling DOI Creative Commons
Maxence Plouviez,

Nicola Brown

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 90, P. 103207 - 103207

Published: Sept. 19, 2024

Language: Английский

Citations

2
Health effects of synthetic additives and the substitution potential of plant-based additives DOI
Jiaqi Chen, Pengguo Xia

Food Research International, Journal Year: 2024, Volume and Issue: 197, P. 115177 - 115177

Published: Oct. 5, 2024

Language: Английский

Citations

1
Phosphorus fertilizer: from commodity to speciality — from fertilizing the field to fertilizing the plant DOI
Sandeep Sharma, P.S. Bindraban, Christian O. Dimkpa

et al.

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 90, P. 103198 - 103198

Published: Sept. 14, 2024

Language: Английский

Citations

1