Oral SARS-CoV-2 Inoculation Causes Nasal Viral Infection Leading to Olfactory Bulb Infection: An Experimental Study DOI Creative Commons
Rumi Ueha, Toshihiro Ito, Ryutaro Furukawa

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2022, Номер 12

Опубликована: Июнь 13, 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections can cause long-lasting anosmia, but the impact of SARS-CoV-2 infection, which spread to nasal cavity via oral route, on olfactory receptor neuron (ORN) lineage and bulb (OB) remains undetermined. Using Syrian hamsters, we explored whether inoculation lead viral examined how affects ORN by site, investigated infection OB induce inflammation. On post-inoculation day 7, presence was confirmed in lateral area (OCAM-positive) not septum NQO1-positive OCAM-positive areas. The virus observed partially infiltrating epithelium, progenitor cells, immature ORNs, mature ORNs were fewer than controls. found nerve bundles OB, suggesting as a route for brain infection. We demonstrated that transoral from central nervous system possibility dysfunction due localized at site could damage all ORN-lineage cells.

Язык: Английский

Increased pathogenicity and aerosol transmission for one SARS-CoV-2 B.1.617.2 Delta variant over the wild-type strain in hamsters DOI Creative Commons

Xinghai Zhang,

Shaohong Chen,

Zengguo Cao

и другие.

Virologica Sinica, Год журнала: 2022, Номер 37(6), С. 796 - 803

Опубликована: Сен. 29, 2022

During the two-year pandemic of coronavirus disease 2019 (COVID-19), its causative agent, severe acute respiratory syndrome 2 (SARS-CoV-2), has been evolving. SARS-CoV-2 Delta, a variant concern, become dominant circulating strain worldwide within just few months. Here, we performed comprehensive analysis new B.1.617.2 Delta (Delta630) compared with early WIV04 (WIV04) in vitro and vivo, terms replication, infectivity, pathogenicity, transmission hamsters. When inoculated intranasally, Delta630 led to more pronounced weight loss Moreover, 40% mortality occurred about one week after infection 104 ​PFU Delta630, whereas no deaths even 105 or other strains belonging variant. outgrew over competitive aerosol experiment. Taken together, showed increased replication ability, transmissibility To our knowledge, this is first that causes death hamster model, which could be an asset for efficacy evaluation vaccines antivirals against infections strains. The underlying molecular mechanisms virulence await further analysis.

Язык: Английский

Процитировано

15
Human Pluripotent Stem Cell–Derived Cardiomyocytes for COVID-19 Cardiovascular Complications: A Versatile In Vitro Model for Studying Acute and Chronic SARS-CoV-2 Infection DOI

Carmen Lopez-Munoz,

Noah Jackson Bowers,

Silvia Marchianò

и другие.

Current Treatment Options in Cardiovascular Medicine, Год журнала: 2025, Номер 27(1)

Опубликована: Фев. 20, 2025

Язык: Английский

Процитировано

0
Review on the current status of Syrian hamsters as an animal model for COVID-19 transmission, pathogenesis, treatment and vaccine research DOI Creative Commons

Biruh Mebratu Belay,

Abebe Tesfaye Gessese, Mebrie Zemene Kinde

и другие.

All Life, Год журнала: 2025, Номер 18(1)

Опубликована: Фев. 21, 2025

Язык: Английский

Процитировано

0
Translational research on pandemic virus infection using nonhuman primate models DOI
Hirohito Ishigaki, Yasushi Itoh

Virology, Год журнала: 2025, Номер unknown, С. 110511 - 110511

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Oral SARS-CoV-2 Inoculation Causes Nasal Viral Infection Leading to Olfactory Bulb Infection: An Experimental Study DOI Creative Commons
Rumi Ueha, Toshihiro Ito, Ryutaro Furukawa

и другие.

Frontiers in Cellular and Infection Microbiology, Год журнала: 2022, Номер 12

Опубликована: Июнь 13, 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections can cause long-lasting anosmia, but the impact of SARS-CoV-2 infection, which spread to nasal cavity via oral route, on olfactory receptor neuron (ORN) lineage and bulb (OB) remains undetermined. Using Syrian hamsters, we explored whether inoculation lead viral examined how affects ORN by site, investigated infection OB induce inflammation. On post-inoculation day 7, presence was confirmed in lateral area (OCAM-positive) not septum NQO1-positive OCAM-positive areas. The virus observed partially infiltrating epithelium, progenitor cells, immature ORNs, mature ORNs were fewer than controls. found nerve bundles OB, suggesting as a route for brain infection. We demonstrated that transoral from central nervous system possibility dysfunction due localized at site could damage all ORN-lineage cells.

Язык: Английский

Процитировано

14