Plant Growth Regulation, Год журнала: 2024, Номер unknown
Опубликована: Дек. 14, 2024
Язык: Английский
BMC Genomics, Год журнала: 2025, Номер 26(1)
Опубликована: Фев. 17, 2025
Reactive oxygen species (ROS) serve as signal molecules in plant defense responses, and the respiratory burst oxidase homolog (RBOH) enzyme plays a crucial role their production. Although numerous RBOH family members have been identified various plants, little is known about genes blueberries. In this study, we six VcRBOH from blueberry genome. Phylogenetic analysis revealed that these can be classified into three subgroups. Conserved domain motif demonstrated high sequence similarity among proteins. Analysis of cis-acting elements suggested may involved stress, light, phytohormone responsiveness. Based on transcriptome data, observed low expression levels VcRBOHB, VcRBOHC, VcRBOHE during flower_at_anthesis stage. contrast, VcRBOHA VcRBOHD showed relatively tissues. The reverse-transcription quantitative PCR (RT-qPCR) indicated rapid induction VcRBOHF by flg22 chitin treatments. Notably, overexpression Arabidopsis promoted PTI including increased marker genes, ROS production, callose deposition. Moreover, resulted enhanced disease resistance against Pseudomonas syringae pv. tomato (Pst) DC3000 infection. These findings provide valuable insights roles responses lay groundwork for more comprehensive understanding molecular mechanisms underpinning resistance.
Язык: Английский
Процитировано
1
Plant Gene, Год журнала: 2024, Номер 38, С. 100451 - 100451
Опубликована: Фев. 15, 2024
Язык: Английский
Процитировано
4
Heliyon, Год журнала: 2024, Номер 10(6), С. e27814 - e27814
Опубликована: Март 1, 2024
Two-pore K+ (TPK) channels are voltage-independent and involved in stress response plants. Herein, we identified 12 TaTPK genes located on nine chromosomes the Triticum aestivum genome. The majority of comprised two exons. Each channel four transmembrane (TM) helices, N- C-terminal ion-channel domains, EF-hand domains one 14-3-3 binding site. Additionally, highly conserved 'GYGD' motif responsible for ion specificity, was found between TMs both domains. Nine were predicted to be localised at plasma membrane, while three vacuolar. protein-protein protein-chemical interactions indicated coordinated functioning with other transporters their possible interaction Ca2+-signaling pathway. Expression studies suggested importance vegetative reproductive tissues development. Significantly modulated expression various during heat, drought salt stresses, after fungal infestation depicted functions response. miRNAs transcription factors analyses role hormone, light, growth development-related stress-responsive signaling cascade. current study vital genes, especially response, would provide an opportunity detailed characterization future studies.
Язык: Английский
Процитировано
4
Agronomy, Год журнала: 2024, Номер 14(4), С. 653 - 653
Опубликована: Март 23, 2024
Plant NAPDH oxidase (NOX) gene family members are critical producers of ROS and play crucial roles in plant signaling, development, stress responses. Opisthopappus taihangensis is a pivotal wild genetic resource Asteraceae. To date, little knowledge exists about the functions NOXs O. taihangensis. In this study, seven typical ferric reduction oxidases (FROs) were identified Dispersed duplication might role expansion NOX/FRO taihangensis, most homologous pairs have undergone purifying selection. Although results collinearity analysis show that these genes relatively conserved, gain loss occurred frequently during evolution Phylogenetic indicated FROs ancient, earliest diverged branch was revealed by maximum likelihood (ML) tree. The Asteraceae divided into six subgroups according to their relationship with Arabidopsis rice members. transcriptome profile unveiled tissue-specific expression patterns complex response modes under drought salt stresses. OtNOX6 OtNOX7 could be recognized as important candidates respond stress. Our provide clues references for further in-depth exploration function other horticulture plants.
Язык: Английский
Процитировано
3
Plants, Год журнала: 2024, Номер 13(3), С. 379 - 379
Опубликована: Янв. 27, 2024
Plants face multiple stresses in their natural habitats. WRKY transcription factors (TFs) play an important regulatory role plant stress signaling, regulating the expression of stress-related genes to improve resistance. In this study, we analyzed profiles 25 BnWRKY three stages ramie growth (the seedling stage, rapid-growth and fiber maturity stage) response abiotic through qRT-PCR. The results indicated that a different were induced by salt drought root leaf. We selected BnWRKY49 as candidate gene for overexpression Arabidopsis. was localized nucleus. Overexpression affected elongation under at Arabidopsis stage exhibited increased tolerance stress. Further research found BnWRKY49-overexpressing lines showed decreased stomatal size cuticular wax deposition compared with wild type (WT). Antioxidant enzyme activities SOD, POD, CAT higher than WT. These findings suggested played laid foundation further on functional analysis BnWRKYs ramie.
Язык: Английский
Процитировано
2
Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 53 - 97
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
2
Journal of Plant Growth Regulation, Год журнала: 2024, Номер unknown
Опубликована: Сен. 19, 2024
Язык: Английский
Процитировано
2
Plant Physiology and Biochemistry, Год журнала: 2024, Номер 217, С. 109281 - 109281
Опубликована: Ноя. 13, 2024
Язык: Английский
Процитировано
2
Plants, Год журнала: 2024, Номер 13(10), С. 1386 - 1386
Опубликована: Май 16, 2024
Pithiness is one of the physiological diseases radishes, which accompanied by accumulation reactive oxygen species (ROS) during sponging parenchyma tissue in fleshy roots. A respiratory burst oxidase homolog (Rboh, also known as NADPH oxidase) a key enzyme that catalyzes production ROS plants. To understand role Rboh genes radish pithiness, herein, 10 RsRboh gene families were identified genome Raphanus sativus using Blastp and Hmmer searching methods subjected to basic functional analyses such phylogenetic tree construction, chromosomal localization, conserved structural domain analysis, promoter element prediction. The expression profiles RsRbohs five stages (Pithiness grade = 0, 1, 2, 3, 4, respectively) pithiness analyzed. results showed expressed different levels development pithiness. Except for RsRbohB RsRbohE, other members increased reached peak at P2 2) stage, among RsRbohD1 highest transcripts. Then, 40 related These can provide theoretical basis improving tolerance radishes.
Язык: Английский
Процитировано
1
Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 1 - 35
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
1