Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Июнь 24, 2024
Infections
caused
by
multidrug-resistant
(MDR)
bacteria
have
become
a
major
challenge
for
global
healthcare
systems.
The
search
antibacterial
compounds
from
plants
has
received
increasing
attention
in
the
fight
against
MDR
bacteria.
As
medicinal
and
edible
plant,
Lophatherum
gracile
Brongn.
(
L.
)
favorable
effect.
However,
main
active
compound
its
antimicrobial
mechanism
are
not
clear.
Here,
our
study
first
identified
key
as
luteolin.
Meanwhile,
effect
of
luteolin
was
detected
using
broth
microdilution
method
time-kill
curve
analysis.
Luteolin
can
also
cause
morphological
structure
degeneration
content
leakage,
cell
wall/membrane
damage,
ATP
synthesis
reduction,
downregulation
mRNA
expression
levels
sulfonamide
quinolones
resistance
genes
Escherichia
coli
(MDR
E.
).
Furthermore,
untargeted
UPLC/Q-TOF-MS-based
metabolomics
analysis
bacterial
metabolites
revealed
that
significantly
changed
riboflavin
energy
metabolism,
chemotaxis
process
glycerophospholipid
metabolism
.
This
suggests
could
be
potential
new
food
additive
or
preservative
controlling
infection
spread.
Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Июнь 20, 2023
Probiotics
are
live
microorganisms
with
various
health
benefits
when
consumed
in
appropriate
amounts.
Fermented
foods
a
rich
source
of
these
beneficial
organisms.
This
study
aimed
to
investigate
the
probiotic
potential
lactic
acid
bacteria
(LAB)
isolated
from
fermented
papaya
(Carica
L.)
through
vitro
methods.
The
LAB
strains
were
thoroughly
characterized,
considering
their
morphological,
physiological,
fermentative,
biochemical,
and
molecular
properties.
strain's
adherence
resistance
gastrointestinal
conditions,
as
well
its
antibacterial
antioxidant
capabilities,
examined.
Moreover,
tested
for
susceptibility
against
specific
antibiotics,
safety
evaluations
encompassed
hemolytic
assay
DNase
activity.
supernatant
isolate
underwent
organic
profiling
(LCMS).
primary
objective
this
was
assess
inhibitory
activity
α-amylase
α-glucosidase
enzymes,
both
silico.
Gram-positive
that
catalase-negative
carbohydrate
fermenting
selected
further
analysis.
exhibited
bile
(0.3%
1%),
phenol
(0.1%
0.4%),
simulated
juice
(pH
3-8).
It
demonstrated
potent
abilities
kanamycin,
vancomycin,
methicillin.
strain
showed
autoaggregation
(83%)
adhesion
chicken
crop
epithelial
cells,
buccal
HT-29
cells.
Safety
assessments
indicated
no
evidence
hemolysis
or
DNA
degradation,
confirming
isolates.
isolate's
identity
confirmed
using
16S
rRNA
sequence.
Levilactobacillus
brevis
RAMULAB52,
derived
papaya,
promising
significant
inhibition
(86.97%)
(75.87%)
enzymes.
In
silico
studies
uncovered
hydroxycitric
acid,
one
acids
isolate,
interacted
crucial
amino
residues
target
Specifically,
formed
hydrogen
bonds
key
residues,
such
GLU233
ASP197
α-amylase,
ASN241,
ARG312,
GLU304,
SER308,
HIS279,
PRO309,
PHE311
α-glucosidase.
conclusion,
possesses
properties
exhibits
an
effective
remedy
diabetes.
Its
abilities,
different
cell
types,
enzymes
make
it
valuable
candidate
research
application
field
probiotics
diabetes
management.
Journal of Functional Foods,
Год журнала:
2023,
Номер
107, С. 105643 - 105643
Опубликована: Июнь 25, 2023
This
study
aimed
to
determine
structure–function
relationships
of
monomeric
and
oligomeric
flavan-3-ols
(procyanidins)
with
respect
their
antimicrobial
activity
inhibition
digestive
enzymes.
Monomeric
flacan-3-ols
were
purchased
as
reference
compounds;
procyanidins
extracted
from
grape
seeds,
separated
by
high-performance
counter-current
chromatography
characterized
LC-MS/MS.
The
against
a
broad
range
intestinal
microorganisms
increased
in
the
order
catechin
=
epicatechin
>
epigallocatechin
gallate.
Facultative
anaerobes
highly
resistant
while
strict
including
Allobaculum
sp.
Ruminococcus
gnavus
10
–
100
times
more
sensitive.
enzymes
rat
small
intestine
<
Trimeric
polymeric
inhibitory
than
dimeric
procyanidins.
Both
enzyme-inhibitory
activities
likely
relate
microbiome-modulating
health
beneficial
properties.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Июнь 24, 2024
Infections
caused
by
multidrug-resistant
(MDR)
bacteria
have
become
a
major
challenge
for
global
healthcare
systems.
The
search
antibacterial
compounds
from
plants
has
received
increasing
attention
in
the
fight
against
MDR
bacteria.
As
medicinal
and
edible
plant,
Lophatherum
gracile
Brongn.
(
L.
)
favorable
effect.
However,
main
active
compound
its
antimicrobial
mechanism
are
not
clear.
Here,
our
study
first
identified
key
as
luteolin.
Meanwhile,
effect
of
luteolin
was
detected
using
broth
microdilution
method
time-kill
curve
analysis.
Luteolin
can
also
cause
morphological
structure
degeneration
content
leakage,
cell
wall/membrane
damage,
ATP
synthesis
reduction,
downregulation
mRNA
expression
levels
sulfonamide
quinolones
resistance
genes
Escherichia
coli
(MDR
E.
).
Furthermore,
untargeted
UPLC/Q-TOF-MS-based
metabolomics
analysis
bacterial
metabolites
revealed
that
significantly
changed
riboflavin
energy
metabolism,
chemotaxis
process
glycerophospholipid
metabolism
.
This
suggests
could
be
potential
new
food
additive
or
preservative
controlling
infection
spread.
